| PRIMARY INFORMATION |
|---|
| ID | 1373 |
| PMID | 21297299 |
| Year | 2011 |
| Sequence | FCIGRLCG |
| Name | AT 1002 analog |
| Length | 8 |
| N-Terminal Modification | Free |
| C-Terminal Modification | Free |
| Linear/ Cyclic | Linear |
| Chirality | L |
| Chemical Modification | None |
| Origin of Peptide | Analogue of AT 1002 |
| Nature of Peptide/Cargo | Cell penetrating peptide |
| Mechanism | Not mentioned |
| Cargo Sequence/Structure | Sense- 5' AUC CGC GCG AUA GUA CGU ATT 3' Antisense- 5' 6-FAM UAC GUA CUA UCG CGC GGA UTT 3' |
Name of cargo
| FAM-labeled siRNA |
| Assay | Confocal laser microscopy |
| Enhancer | Skin was shaved |
| Properties of enhancer | Hairless skin eases transdermal delivery |
| Concentration | 5 µg siRNA+ 400 µg peptide |
| Incubation time | 10 hours |
| Tissue permeability (value with units) | Fluorescent signals of FAMsiRNA in the AT1002 applied skin were observed faintly at the stratum corneum in 10 times tape-stripped skin. In 20 times tape-stripped skin FAMsiRNA was observed widely and strikingly at the stratum corneum, hair follicle, epidermal and dermal layers. |
| Tissue Sample | Tape stripped skin of female ICR mice |
| Ex vivo/In vivo/In vitro | in vivo |
| SECONDARY INFORMATION |
|---|
| STRUCTURE | |
| SMILES | N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CS)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)
N[C@@H](CCCNC(=[NH2])N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CS)C(=O)NCC=O |
