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Details of TopicalPdb ID 1370

PRIMARY INFORMATION
ID	1370
PMID	21297299
Year	2011
Sequence	GRKKRRQRRRCG
Name	Tat analog
Length	12
N-Terminal Modification	Free
C-Terminal Modification	Free
Linear/ Cyclic	Linear
Chirality	L
Chemical Modification	None
Origin of Peptide	Analogue of Tat
Nature of Peptide/Cargo	Cell penetrating peptide
Mechanism	Not mentioned
Cargo Sequence/Structure	Sense-5' GGU GCA GAA AGA AGA CAU UTT 3' Antisense-5' AAU GUC UUC UUU CUG CAC CTT 3'
Name of cargo	siRelA
Assay	Real-time PCR
Enhancer	None
Properties of enhancer	Not applicable
Concentration	100µl containing siRelA: 0.05 mg Tat (N/P ratio of Tat/siRelA 1:10)
Incubation time	24 hours
Tissue permeability (value with units)	A high value of silencing was seen in presence of Tat/siRelA (N/P=10) in PAM212 cells.
Tissue Sample	PAM212 cells (mouse keratinocytes)
Ex vivo/In vivo/In vitro	in vitro
SECONDARY INFORMATION
STRUCTURE	Predicted Structure
SMILES	NCC(=O)N[C@@H](CCCNC(=[NH2])N)C(=O)N[C@@H](CCCC[NH3])C (=O)N[C@@H](CCCC[NH3])C(=O)N[C@@H](CCCNC(=[NH2])N)C(=O)N[C@@H](CCCNC(=[NH2])N)C (=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCNC(=[NH2])N)C(=O)N[C@@H](CCCNC (=[NH2])N)C(=O)N[C@@H](CCCNC(=[NH2])N)C(=O)N[C@@H](CS)C(=O)NCC=O