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Details of TopicalPdb ID 1369

PRIMARY INFORMATION
ID1369
PMID20891012
Year2010
SequenceGRKKRRQRRRPPQRKC
MSKGEELFTGVVPILV
ELDGDVNGHKFSVSGE
GEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTF
SYGVQCFSRYPDHMKQ
HDFFKSAMPEGYVQE
RTIFFKDDGNYKTRAE
VKFEGDTLVNRIELKG
IDFKEDGNILGHKLEY
NYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGS
VQLADHYQQNTPIGDG
PVLLPDNHYLSTQSAL
SKDPNEKRDHMVLLEF
NameN-terminal Tat fusion green fluorescent protein (GFP) (TG)
Length254
N-Terminal ModificationFree
C-Terminal ModificationFree
Linear/ CyclicLinear
ChiralityL
Chemical ModificationNone
Origin of PeptideSynthetic fusion protein of TAT and GFP
Nature of Peptide/CargoFluorescent CPP
MechanismNot mentioned
Cargo Sequence/StructureNone
Name of cargo
Not applicable
AssayVertical Franz diffusion cell
EnhancerTG loaded in elastic anionic niosomes (Tween 61/CHL/DP at 1:1:0.05 molar ratio) containing 1 mol% of NaC
Properties of enhancerThe enhancement of cellular internalization of might be due to the ethanol or edge activators (NaC/NaDC) composition in the elastic niosomal formulation
Concentration10µM
Incubation time6 hours
Tissue permeability (value with units)Cumulative amounts 8.59 ± 1.33 µg/cm2 and fluxes 1.43 ± 0.22 µg/cm2·h for stratum corneum. The receiver compartment of Franz Diffusion Cells had cumulative amount=62.75 ± 2.68 µg/cm2 and fluxes= 10.46 ± 3.45 µg/cm2·h
Tissue SampleStratum corneum of the abdominal skin of Sparague-Dawley rats after shaving off hair
Ex vivo/In vivo/In vitroex vivo
SECONDARY INFORMATION
STRUCTURE
Predicted Structure
SMILESN.A.