ID | 1366 | |
PMID | 20891012 | |
Year | 2010 | |
Sequence | GRKKRRQRRRPPQRKC MSKGEELFTGVVPILV ELDGDVNGHKFSVSGE GEGDATYGKLTLKFIC TTGKLPVPWPTLVTTF SYGVQCFSRYPDHMKQ HDFFKSAMPEGYVQE RTIFFKDDGNYKTRAE VKFEGDTLVNRIELKG IDFKEDGNILGHKLEY NYNSHNVYIMADKQKN GIKVNFKIRHNIEDGS VQLADHYQQNTPIGDG PVLLPDNHYLSTQSAL SKDPNEKRDHMVLLEF | |
Name | N-terminal Tat fusion green fluorescent protein (GFP) (TG) | |
Length | 254 | |
N-Terminal Modification | Free | |
C-Terminal Modification | Free | |
Linear/ Cyclic | Linear | |
Chirality | L | |
Chemical Modification | None | |
Origin of Peptide | Synthetic fusion protein of TAT and GFP | |
Nature of Peptide/Cargo | Fluorescent CPP | |
Mechanism | Not mentioned | |
Cargo Sequence/Structure | None | |
Name of cargo | Not applicable | |
Assay | Vertical Franz diffusion cell | |
Enhancer | GFP solution in 100 mM phosphate buffer (pH 7.0) | |
Properties of enhancer | Not mentioned | |
Concentration | 10µM | |
Incubation time | 6 hours | |
Tissue permeability (value with units) | Cumulative amounts 6.06 ± 0.70 µg/cm2 and fluxes 1.01 ± 0.70 µg/cm2·h for viable epidermis and dermis of skin. | |
Tissue Sample | Viable epidermis and dermis of the abdominal skin of Sparague-Dawley rats after shaving off hair | |
Ex vivo/In vivo/In vitro | ex vivo | |
STRUCTURE |
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SMILES | N.A. |