ID | 1364 | |
PMID | 20891012 | |
Year | 2010 | |
Sequence | GRKKRRQRRRPPQRKC MSKGEELFTGVVPILV ELDGDVNGHKFSVSGE GEGDATYGKLTLKFIC TTGKLPVPWPTLVTTF SYGVQCFSRYPDHMKQ HDFFKSAMPEGYVQE RTIFFKDDGNYKTRAE VKFEGDTLVNRIELKG IDFKEDGNILGHKLEY NYNSHNVYIMADKQKN GIKVNFKIRHNIEDGS VQLADHYQQNTPIGDG PVLLPDNHYLSTQSAL SKDPNEKRDHMVLLEF | |
Name | N-terminal Tat fusion green fluorescent protein (GFP) (TG) | |
Length | 254 | |
N-Terminal Modification | Free | |
C-Terminal Modification | Free | |
Linear/ Cyclic | Linear | |
Chirality | L | |
Chemical Modification | None | |
Origin of Peptide | Synthetic fusion protein of TAT and GFP | |
Nature of Peptide/Cargo | Fluorescent CPP | |
Mechanism | Not mentioned | |
Cargo Sequence/Structure | None | |
Name of cargo | Not applicable | |
Assay | Vertical Franz diffusion cell | |
Enhancer | GFP solution in 100 mM phosphate buffer (pH 7.0) | |
Properties of enhancer | Not mentioned | |
Concentration | 10µM | |
Incubation time | 6 hours | |
Tissue permeability (value with units) | Cumulative amounts 4.86 ± 0.55 µg/cm2 and fluxes 0.81 ± 0.09 µg/cm2·h for stratum corneum. The receiver compartment of Franz Diffusion Cells had cumulative amount=28.50 ± 12.07 µg/cm2 and fluxes= 4.75 ± 1.47 µg/cm2·h | |
Tissue Sample | Stratum corneum of the abdominal skin of Sparague-Dawley rats after shaving off hair | |
Ex vivo/In vivo/In vitro | ex vivo | |
STRUCTURE |
| |
SMILES | N.A. |