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Details of TopicalPdb ID 1300

PRIMARY INFORMATION
ID1300
PMID7646536
Year1995
SequenceHDMNKVLDL
NameNonapeptide P2 or AF-2
Length9
N-Terminal ModificationFree
C-Terminal ModificationFree
Linear/ CyclicLinear
ChiralityL
Chemical ModificationNone
Origin of PeptideCorresponds to the 246-254 sequence of lipocortin I
Nature of Peptide/CargoShowed an anti-inflammatory effect in carrageenan-induced rat paw oedema, they inhibit pancreatic and Naja naja PLA2 in vitro and acute inflammatory processes induced by carrageenan or phorbol esters when administered locally or parenterally.
MechanismThe anti-inflammatory effects of AF-2 in the TPA model suggest that this nonapeptide could affect arachidonic acid mobilization and/or arachidonic acid metabolism by 5-lipoxygenase.
Cargo Sequence/StructureNone
Name of cargo
Not applicable
AssayMPO and NAG assays
EnhancerAF-2 is dissolved in Tris-HCl 10mM pH 8.0 buffer.
Properties of enhancerNot mentioned
Concentration20 µl/ear 5 min before TPA(10 µg/ear)(100µg/ear) application
Incubation time6 hours
Tissue permeability (value with units)MPO levels(mU/ear)=Untreated(0.02)/Treated(38.2 ± 2.5), NAG levels(mU/ear)=Untreated(23.1 ± 1.1)/Treated(27.1 ± 2.6), 6-keto-PGF1α(pg/ear)=Untreated(198 ± 22)/Treated(254 ± 26), LTB4 levels(pg/ear)=Untreated(0.30)/Treated(523 ± 128)
Tissue SampleEar(auditory pinna) of male Swiss Webster mice
Ex vivo/In vivo/In vitroin vivo
SECONDARY INFORMATION
STRUCTURE
Predicted Structure
SMILESN[C@@H](Cc1nc[nH]c1)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC
(=O)N)C(=O)N[C@@H](CCCC[NH3])C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)
C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(C)C)C=O