ID | 1295 | |
PMID | 7646536 | |
Year | 1995 | |
Sequence | HDMNKVLDL | |
Name | Nonapeptide P2 or AF-2 | |
Length | 9 | |
N-Terminal Modification | Free | |
C-Terminal Modification | Free | |
Linear/ Cyclic | Linear | |
Chirality | L | |
Chemical Modification | None | |
Origin of Peptide | Corresponds to the 246-254 sequence of lipocortin I | |
Nature of Peptide/Cargo | Showed an anti-inflammatory effect in carrageenan-induced rat paw oedema, they inhibit pancreatic and Naja naja PLA2 in vitro and acute inflammatory processes induced by carrageenan or phorbol esters when administered locally or parenterally. | |
Mechanism | The anti-inflammatory effects of AF-2 in the TPA model suggest that this nonapeptide could affect arachidonic acid mobilization and/or arachidonic acid metabolism by 5-lipoxygenase. | |
Cargo Sequence/Structure | None | |
Name of cargo | Not applicable | |
Assay | MPO and NAG assays | |
Enhancer | AF-2 is dissolved in Tris-HCl 10mM pH 8.0 buffer. | |
Properties of enhancer | Not mentioned | |
Concentration | 20 µl/ear 5 min before AA(2 mg/ear)(25µg/ear) application | |
Incubation time | 30 minutes | |
Tissue permeability (value with units) | MPO levels(mU/ear)=Untreated(0.03)/Treated(1.72 ± 0.20), PGE2 levels(ng/ear)=Untreated(0)/Treated(83.2 ± 2.8), LTB4 levels(ng/ear)=Untreated(0)/Treated(15.2 ± 1.2) | |
Tissue Sample | Ear(auditory pinna) of male Swiss Webster mice | |
Ex vivo/In vivo/In vitro | in vivo | |
STRUCTURE |
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SMILES | N[C@@H](Cc1nc[nH]c1)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC (=O)N)C(=O)N[C@@H](CCCC[NH3])C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C) C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(C)C)C=O |