Welcome to Entry Card of Peptide


Details of TopicalPdb ID 1258

PRIMARY INFORMATION
ID1258
PMID8584255
Year1995
SequenceVTHRLAGLLSRSGGM
VKSNFVPTNVGSKAF
NameCGRP receptor antagonist CGRP(8-37)
Length30
N-Terminal ModificationFree
C-Terminal ModificationAmidation
Linear/ CyclicLinear
ChiralityL
Chemical ModificationNone
Origin of PeptideSynthetic
Nature of Peptide/CargoRegulation of blood flow, modulation of inflammation, and tissue remodelling
MechanismIncreases proliferation of human umbilical vein endothelial cells and therefore may contribute to the formation of new vessels during regeneration. CGRP increased the survival of musculocutaneous critical flaps in the rat and also stimulated DNA synthesis and proliferation in a human keratinocyte cell line.
Cargo Sequence/StructureNone
Name of cargo
Not applicable
AssayPhotographed both hind paws using a micro-computer imaging device(MCID)-assessed analysis system. Immunostaining. Mann-Whitney U-test.
EnhancerIrradiation intensity of 10.8 J/cm 2 (8.9 mW/s for 20 min). Epicutaneous application of CGRP to the inflamed skin area in 12 h intervals starting epicutaneously to the inflamed skin area in 12 h intervals starting immediately after UV-exposure for 5 successive days.
Properties of enhancerNot applicable
ConcentrationThe CGRP receptor antagonist CGRP(8-37) was dissolved in 1 ml triflouracetic acid (0.1%) and diluted in propylene glycol to obtain a concentration of 50 µM, whose 50 µl was used for topical application.
Incubation time30 days
Tissue permeability (value with units)Effect of the CGRP-receptor antagonist CGRP(8-37)(~7 mm2) on the necrotic area in UV-damaged skin of the rat hindpaw compared to solvent applications(~13.5 mm2).
Tissue SampleThe dorsum of both hind paws were irradiated of male Sprague-Dawley (SD) rats.
Ex vivo/In vivo/In vitroin vivo
SECONDARY INFORMATION
STRUCTURE
Predicted Structure
SMILESN[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C
(=O)NCC(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)NCC(=O)
NCC(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C
(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C
(=O)NCC(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C=O)Cc1ccccc1)C)
CCCCN)CO)C(C)C)CC(=O)N)[C@H](O)C)CCC)C(C)C)Cc1ccccc1)CC(=O)N)CO)
CCCCN)C(C)C)CCSC)CO)CCCNC(=N)N)CO)CC(C)C)CC(C)C)C)CC(C)C)
CCCNC(=N)N)Cc1[nH]cnc1)[C@H](O)C)C(C)C