| ID | 1254 | |
| PMID | 8584255 | |
| Year | 1995 | |
| Sequence | VTHRLAGLLSRSGGM VKSNFVPTNVGSKAF | |
| Name | CGRP receptor antagonist CGRP(8-37) | |
| Length | 30 | |
| N-Terminal Modification | Free | |
| C-Terminal Modification | Amidation | |
| Linear/ Cyclic | Linear | |
| Chirality | L | |
| Chemical Modification | None | |
| Origin of Peptide | Synthetic | |
| Nature of Peptide/Cargo | Regulation of blood flow, modulation of inflammation, and tissue remodelling | |
| Mechanism | Increases proliferation of human umbilical vein endothelial cells and therefore may contribute to the formation of new vessels during regeneration. CGRP increased the survival of musculocutaneous critical flaps in the rat and also stimulated DNA synthesis and proliferation in a human keratinocyte cell line. | |
| Cargo Sequence/Structure | None | |
| Name of cargo | Not applicable | |
| Assay | Photographed both hind paws using a micro-computer imaging device(MCID)-assessed analysis system. Immunostaining. Mann-Whitney U-test. | |
| Enhancer | Irradiation intensity of 10.8 J/cm 2 (8.9 mW/s for 20 min). Epicutaneous application of CGRP to the inflamed skin area in 12 h intervals starting epicutaneously to the inflamed skin area in 12 h intervals starting immediately after UV-exposure for 5 successive days. | |
| Properties of enhancer | Not applicable | |
| Concentration | The CGRP receptor antagonist CGRP(8-37) was dissolved in 1 ml triflouracetic acid (0.1%) and diluted in propylene glycol to obtain a concentration of 50 µM, whose 50 µl was used for topical application. | |
| Incubation time | 7 days | |
| Tissue permeability (value with units) | Effect of the CGRP-receptor antagonist CGRP(8-37)(~24.5 mm2) on the necrotic area in UV-damaged skin of the rat hindpaw compared to solvent applications(~36.5 mm2). | |
| Tissue Sample | The dorsum of both hind paws were irradiated of male Sprague-Dawley (SD) rats. | |
| Ex vivo/In vivo/In vitro | in vivo | |
| STRUCTURE |
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| SMILES | N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C (=O)NCC(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)NCC(=O) NCC(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C (=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C (=O)NCC(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C=O)Cc1ccccc1)C) CCCCN)CO)C(C)C)CC(=O)N)[C@H](O)C)CCC)C(C)C)Cc1ccccc1)CC(=O)N)CO) CCCCN)C(C)C)CCSC)CO)CCCNC(=N)N)CO)CC(C)C)CC(C)C)C)CC(C)C) CCCNC(=N)N)Cc1[nH]cnc1)[C@H](O)C)C(C)C | |