Details of TopicalPdb ID 1126
| ID | 1126 | |
| PMID | 18670091 | |
| Year | 2008 | |
| Sequence | MNFVMISIELSETMAL IIVISGLLLCASSITT TSSNDFYAVLHGNLSQ SDRNELPKICGREFLT DTLQSRSAGGVLVREN EYPWVLLLTDPEWTRV CTAVLISRRHVLTAAH CVTNFPKDRKLEKDCH YTTIQSTYLYVYPRTR VNDALNIKRYTSSFSV ARVMVHPSFSCSNATG DIALLELTLNIFTEAS PICMPHFNESIPKNAA AAGFGKNPISNNTRPM QVVNLTYQGTTGDRI | |
| Name | Trypsin | |
| Length | 293 | |
| N-Terminal Modification | Free | |
| C-Terminal Modification | Free | |
| Linear/ Cyclic | Linear | |
| Chirality | L | |
| Chemical Modification | None | |
| Origin of Peptide | Bovine | |
| Nature of Peptide/Cargo | Endogenous proteases trypsin is capable of digesting intercellular desmosomal proteins and hence used for in vitro epidermal separation and keratinocyte isolation | |
| Mechanism | The unique ability of proteases to cause selective epidermal separation has been in part explained by the proteolytic degradation of desmosomal proteins in the SC, which leads to cell dissociation | |
| Cargo Sequence/Structure | MALWTRLAPLLALLALWAPAPARAFVNQHLCGSHLVEALYLVCGERGFFYTPKARREVEGQVGALELAGGPGAGGLEGPPQKRGIVEQCCASVCSLYQLENYCN | |
| Name of cargo | Bovine insulin | |
| Assay | Plasma glucose level (PGL) determination | |
| Enhancer | None | |
| Properties of enhancer | Not applicable | |
| Concentration | 200 µl of 2.5% trypsin (w/v) | |
| Incubation time | 2 hour time interval upto 8 hours | |
| Tissue permeability (value with units) | Marked decrease in the PGL was observed in the group with trypsin pretreatment. The PGL was reduced to less than 60% of the initial value after 8 h in all groups with trypsin pretreatment | |
| Tissue Sample | Blood collected from the tail vein of diabetic rat | |
| Ex vivo/In vivo/In vitro | in vivo | |
| STRUCTURE |
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| SMILES | N.A. | |