NPACT- Naturally occuring Plant based Anticancerous Compound-Activity-Target DataBase

Compound: Andrographolide

TargetsGene NameGene IDCancerCell LinesIC50ED50EC50GI50RemarkReferences
BAXBCL2-associated X protein581HepatomaHep G2----Bax conformational changes were proven to be caspase 8-dependent, as pretreatment with caspase 8 inhibitor (z-IETD-FMK) almost completely abolished the Andro-induced conformational changes of Bax16740251
BIDBH3 interacting domain death agonist637HepatomaHep G2----When cells were pretreated with pan-caspase inhibitor (z-VAD-FMK) or caspase 8 inhibitor (z-IETD-FMK), the decrease of full length of Bid caused by Andro was prevented,suggesting that Bid cleavage is mediated by caspase 8 activation.16740251
CASP3caspase 3, apoptosis-related cysteine peptidase836HepatomaHep G2----Around eight-fold increases of caspase 3 activity were observed after treatment with Andrographolide for 12 h.16740251
CASP7caspase 7, apoptosis-related cysteine peptidase840HepatomaHep G2----Around eight-fold increases of caspase 7 activity were observed after treatment with Andrographolide for 12 h.16740251
CASP8caspase 8, apoptosis-related cysteine peptidase841HepatomaHep G2----Andro induced apoptosis in human cancer cells via activation of caspase 8 in the extrinsic death receptor pathway16740251
CDK4cyclin-dependent kinase 41019Breast adenocarcinomaMCF-7----Andrographolide decreased the CDK4 protein levels.14641821
CDK4cyclin-dependent kinase 41019Breast adenocarcinomaMCF-7----Induction of decrease in CDK4 expression by andrographolide were time dependent and significant even at 10h.10679837
IL2 interleukin 23558Breast adenocarcinomaMCF-7----Andrographolide treatment enhanced the production of cytokines interlukin-2.10679837
PSMD9proteasome (prosome, macropain) 26S subunit, non-ATPase, 95715Breast adenocarcinomaMCF-7----After andrographolide treatment we also observed the increased proliferation of HPBLs and the increased expression of CD3, CD4, CD8, and CD56 markers on lymphocytes14641821
PSMD9proteasome (prosome, macropain) 26S subunit, non-ATPase, 95715Breast adenocarcinomaMCF-7----Andrographolide induced the expression of p27 which is a major CDK inhibitor involved in the regulation of G1 phase of cell cycle.10679837
TNFtumor necrosis factor7124Breast adenocarcinomaMCF-7----Andrographolide treatment enhanced the production of tumor necrosis factor-alpha10679837

 






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