NPACT- Naturally occuring Plant based Anticancerous Compound-Activity-Target DataBase

Compound: nimbolide

TargetsGene NameGene IDCancerCell LinesIC50ED50EC50GI50RemarkReferences
APAF1apoptotic peptidase activating factor 1317Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression. Quantifi cation of each band by densitometric analysis revealed signifi cant increase in the expression of p53,p21, I k B, Bid, Bad, Bax, cytosolic cytochrome C,Apaf-1, caspase -2L, -6, -8, -9 and -3 and PARP cleavage.20429769
BADBCL2-associated agonist of cell death572Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression. Quantifi cation of each band by densitometric analysis revealed signifi cant increase in the expression of p53,p21, I k B, Bid, Bad, Bax, cytosolic cytochrome C,Apaf-1, caspase -2L, -6, -8, -9 and -3 and PARP cleavage.20429769
BAXBCL2-associated X protein581Colon cancerHCT-116----Nimbolide up-regulated the expression of Bax and cytochrome c in a dose-dependent manner. Levels of Bid, a pro-apoptotic Bcl-2 family protein that is cleaved by caspase-8 to truncated Bid was also decreased in a dose-dependent manner.These results suggest that nimbolide can up-regulate pro-apoptotic protein.21078664
BAXBCL2-associated X protein581Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression. Quantifi cation of each band by densitometric analysis revealed signifi cant increase in the expression of p53,p21, I k B, Bid, Bad, Bax, cytosolic cytochrome C,Apaf-1, caspase -2L, -6, -8, -9 and -3 and PARP cleavage.20429769
BCL2B-cell CLL/lymphoma 2596Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression.Quantifi cation of each band by densitometric analysis revealed signifi cant. with decrease in the expression of cyclin D1,PCNA, NF- k B, p-I k B- a , IKK b , survivin, Bcl-2, Bcl-xL, Mcl-1 and mitochondrial cytochrome c in HeLa cells treated with azadirachtin and nimbolide compared to untreated control.20429769
BCL2B-cell CLL/lymphoma 2596Colon cancerHCT-116----We examined whether nimbolide can modulate the expression of these proteins. Our results revealed that nimbolide down-regulated the expression of Bcl-2,Bcl-xL, cIAP-1, cIAP-2, I-FLICE, survivin, and XIAP. The effect of nimbolide was most prominent at 5 and 10 M. Overall, these results suggest that nimbolide can down-regulate cell survival protein.21078664
BCL2L1BCL2-like 1598Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression.Quantifi cation of each band by densitometric analysis revealed signifi cant. with decrease in the expression of cyclin D1,PCNA, NF- k B, p-I k B- a , IKK b , survivin, Bcl-2, Bcl-xL, Mcl-1 and mitochondrial cytochrome c in HeLa cells treated with azadirachtin and nimbolide compared to untreated control.20429769
BCL2L1BCL2-like 1598Colon cancerHCT-116----We examined whether nimbolide can modulate the expression of these proteins. Our results revealed that nimbolide down-regulated the expression of Bcl-2,Bcl-xL, cIAP-1, cIAP-2, I-FLICE, survivin, and XIAP. The effect of nimbolide was most prominent at 5 and 10 M. Overall, these results suggest that nimbolide can down-regulate cell survival protein.21078664
BIDBH3 interacting domain death agonist637Colon cancerHCT-116----Nimbolide up-regulated the expression of Bax and cytochrome c in a dose-dependent manner. Levels of Bid, a pro-apoptotic Bcl-2 family protein that is cleaved by caspase-8 to truncated Bid was also decreased in a dose-dependent manner.These results suggest that nimbolide can up-regulate pro-apoptotic protein.21078664
BIDBH3 interacting domain death agonist637Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression. Quantifi cation of each band by densitometric analysis revealed signifi cant increase in the expression of p53,p21, I k B, Bid, Bad, Bax, cytosolic cytochrome C,Apaf-1, caspase -2L, -6, -8, -9 and -3 and PARP cleavage.20429769
BIRC2baculoviral IAP repeat containing 2329Colon cancerHCT-116----We examined whether nimbolide can modulate the expression of these proteins. Our results revealed that nimbolide down-regulated the expression of Bcl-2,Bcl-xL, cIAP-1, cIAP-2, I-FLICE, survivin, and XIAP. The effect of nimbolide was most prominent at 5 and 10 M. Overall, these results suggest that nimbolide can down-regulate cell survival protein.21078664
BIRC3baculoviral IAP repeat containing 3 330Colon cancerHCT-116----We examined whether nimbolide can modulate the expression of these proteins. Our results revealed that nimbolide down-regulated the expression of Bcl-2,Bcl-xL, cIAP-1, cIAP-2, I-FLICE, survivin, and XIAP. The effect of nimbolide was most prominent at 5 and 10 M. Overall, these results suggest that nimbolide can down-regulate cell survival protein.21078664
birc5abaculoviral IAP repeat-containing 5a373110Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression.Quantifi cation of each band by densitometric analysis revealed signifi cant. with decrease in the expression of cyclin D1,PCNA, NF- k B, p-I k B- a , IKK b , survivin, Bcl-2, Bcl-xL, Mcl-1 and mitochondrial cytochrome c in HeLa cells treated with azadirachtin and nimbolide compared to untreated control.20429769
birc5abaculoviral IAP repeat-containing 5a373110Colon cancerHCT-116----We examined whether nimbolide can modulate the expression of these proteins. Our results revealed that nimbolide down-regulated the expression of Bcl-2,Bcl-xL, cIAP-1, cIAP-2, I-FLICE, survivin, and XIAP. The effect of nimbolide was most prominent at 5 and 10 M. Overall, these results suggest that nimbolide can down-regulate cell survival protein.21078664
CASP2caspase 2, apoptosis-related cysteine peptidase835Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression. Quantifi cation of each band by densitometric analysis revealed signifi cant increase in the expression of p53,p21, I k B, Bid, Bad, Bax, cytosolic cytochrome C,Apaf-1, caspase -2L, -6, -8, -9 and -3 and PARP cleavage.20429769
CASP3caspase 3, apoptosis-related cysteine peptidase836Colon cancerHCT-116----Activation of caspase-3 suggests that nimbolide potentiated both extrinsic and intrinsic pathways of apoptosis.21078664
CASP3caspase 3, apoptosis-related cysteine peptidase836Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression. Quantifi cation of each band by densitometric analysis revealed signifi cant increase in the expression of p53,p21, I k B, Bid, Bad, Bax, cytosolic cytochrome C,Apaf-1, caspase -2L, -6, -8, -9 and -3 and PARP cleavage.20429769
CASP6caspase 6, apoptosis-related cysteine peptidase 839Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression. Quantifi cation of each band by densitometric analysis revealed signifi cant increase in the expression of p53,p21, I k B, Bid, Bad, Bax, cytosolic cytochrome C,Apaf-1, caspase -2L, -6, -8, -9 and -3 and PARP cleavage.20429769
CASP8caspase 8, apoptosis-related cysteine peptidase841Colon cancerHCT-116----Activation of caspase-8 suggests that nimbolide potentiated both extrinsic and intrinsic pathways of apoptosis.21078664
CASP8caspase 8, apoptosis-related cysteine peptidase841Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression. Quantifi cation of each band by densitometric analysis revealed signifi cant increase in the expression of p53,p21, I k B, Bid, Bad, Bax, cytosolic cytochrome C,Apaf-1, caspase -2L, -6, -8, -9 and -3 and PARP cleavage.20429769
CASP9caspase 9, apoptosis-related cysteine peptidase842Colon cancerHCT-116----Activation of caspase-9 suggests that nimbolide potentiated both extrinsic and intrinsic pathways of apoptosis.21078664
CASP9caspase 9, apoptosis-related cysteine peptidase842Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression. Quantifi cation of each band by densitometric analysis revealed signifi cant increase in the expression of p53,p21, I k B, Bid, Bad, Bax, cytosolic cytochrome C,Apaf-1, caspase -2L, -6, -8, -9 and -3 and PARP cleavage.20429769
CCNE1cyclin E1898Cervix AdenocarcinomaHeLa----HT-29 cells exposed to nimbolide showed down-regulation of cyclin E20429769
CCNE1cyclin E1898Colon CancerHT-29----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression.Quantifi cation of each band by densitometric analysis revealed signifi cant. with decrease in the expression of cyclin D1,PCNA, NF- k B, p-I k B- a , IKK b , survivin, Bcl-2, Bcl-xL, Mcl-1 and mitochondrial cytochrome c in HeLa cells treated with azadirachtin and nimbolide compared to untreated control.20429769
CDK2cyclin-dependent kinase 21017Colon CancerHT-29----HT-29 cells exposed to nimbolide showed down-regulation of cdk220429769
CDKN1Acyclin-dependent kinase inhibitor 1A (p21, Cip1)1026Colon CancerHT-29----HT-29 cells exposed to nimbolide showed G2/M phase arrest, up-regulation of p21.20429769
CDKN1Acyclin-dependent kinase inhibitor 1A (p21, Cip1)1026Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression. Quantifi cation of each band by densitometric analysis revealed signifi cant increase in the expression of p53,p21, I k B, Bid, Bad, Bax, cytosolic cytochrome C,Apaf-1, caspase -2L, -6, -8, -9 and -3 and PARP cleavage.20429769
CFLARCASP8 and FADD-like apoptosis regulator8837Colon cancerHCT-116----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression. Quantifi cation of each band by densitometric analysis revealed signifi cant increase in the expression of p53,p21, I k B, Bid, Bad, Bax, cytosolic cytochrome C,Apaf-1, caspase -2L, -6, -8, -9 and -3 and PARP cleavage.21078664
CHEK-2checkpoint kinase 211200Colon CancerHT-29----HT-29 cells exposed to nimbolide showed up-regulation of chk220429769
CycACyclin A39340Colon CancerHT-29----HT-29 cells exposed to nimbolide showed down-regulation of cyclin A.20429769
CYCScytochrome c54205Cervix AdenocarcinomaHeLa----Quantification by densitometric analysis revealed significant increase in the expression of cytosolic cytochrome C, and mitochondrial cytochrome c in HeLa cells treated with nimbolide compared to untreated control. 20429769
CYCScytochrome c54205Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression. Quantifi cation of each band by densitometric analysis revealed signifi cant increase in the expression of p53,p21, I k B, Bid, Bad, Bax, cytosolic cytochrome C,Apaf-1, caspase -2L, -6, -8, -9 and -3 and PARP cleavage.20429769
IKBKBinhibitor of kappa light polypeptide gene enhancer in B-cells3551Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression.Quantifi cation of each band by densitometric analysis revealed signifi cant. with decrease in the expression of cyclin D1,PCNA, NF- k B, p-I k B- a , IKK b , survivin, Bcl-2, Bcl-xL, Mcl-1 and mitochondrial cytochrome c in HeLa cells treated with azadirachtin and nimbolide compared to untreated control.20429769
IKBKEinhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon9641Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression.Quantifi cation of each band by densitometric analysis revealed signifi cant. with decrease in the expression of cyclin D1,PCNA, NF- k B, p-I k B- a , IKK b , survivin, Bcl-2, Bcl-xL, Mcl-1 and mitochondrial cytochrome c in HeLa cells treated with azadirachtin and nimbolide compared to untreated control.20429769
MCL1myeloid cell leukemia sequence 1 (BCL2-related)4170Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression.Quantifi cation of each band by densitometric analysis revealed signifi cant. with decrease in the expression of cyclin D1,PCNA, NF- k B, p-I k B- a , IKK b , survivin, Bcl-2, Bcl-xL, Mcl-1 and mitochondrial cytochrome c in HeLa cells treated with azadirachtin and nimbolide compared to untreated control.20429769
NFKB1nuclear factor of kappa light polypeptide gene enhancer in B-cells 14790Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression.Quantifi cation of each band by densitometric analysis revealed signifi cant. with decrease in the expression of cyclin D1,PCNA, NF- k B, p-I k B- a , IKK b , survivin, Bcl-2, Bcl-xL, Mcl-1 and mitochondrial cytochrome c in HeLa cells treated with azadirachtin and nimbolide compared to untreated control.20429769
NFKBIAnuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha4792Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression.Quantifi cation of each band by densitometric analysis revealed signifi cant. with decrease in the expression of cyclin D1,PCNA, NF- k B, p-I k B- a , IKK b , survivin, Bcl-2, Bcl-xL, Mcl-1 and mitochondrial cytochrome c in HeLa cells treated with azadirachtin and nimbolide compared to untreated control.20429769
PARP1poly (ADP-ribose) polymerase 1142Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression. Quantifi cation of each band by densitometric analysis revealed signifi cant increase in the expression of p53,p21, I k B, Bid, Bad, Bax, cytosolic cytochrome C,Apaf-1, caspase -2L, -6, -8, -9 and -3 and PARP cleavage.20429769
PCNAproliferating cell nuclear antigen5111Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression.Quantifi cation of each band by densitometric analysis revealed signifi cant. with decrease in the expression of cyclin D1,PCNA, NF- k B, p-I k B- a , IKK b , survivin, Bcl-2, Bcl-xL, Mcl-1 and mitochondrial cytochrome c in HeLa cells treated with azadirachtin and nimbolide compared to untreated control.20429769
RAD17RAD17 homolog (S. pombe)5884Colon CancerHT-29----HT-29 cells exposed to nimbolide showed down-regulation of Rad17.20429769
ROS1c-ros oncogene 1 , receptor tyrosine kinase6098Cervix AdenocarcinomaHeLa----There is accumulating evidence to indicate that ROS plays an important role in apoptosis induction.Treatment with azadirachtin (135 µ M) and nimbolide (5 µ M) for 24 h significantly increased ROS generation in HeLa cells compared to control.20429769
TNFRSF10Atumor necrosis factor receptor superfamily, member 10a8797Colon cancerHCT-116----The limonoid up-regulated DR4 in HCT-116 cells in a dose- and time-dependent manner . Treatment of cells with 5 M nimbolide for 12 h was found optimal for up-regulating DRs without affecting cell viabilit.21078664
TNFRSF10Btumor necrosis factor receptor superfamily, member 10b8795Colon cancerHCT-116----The limonoid up-regulated DR5 in HCT-116 cells in a dose- and time-dependent manner . Treatment of cells with 5 M nimbolide for 12 h was found optimal for up-regulating DRs without affecting cell viabilit.21078664
TNFRSF10Btumor necrosis factor receptor superfamily, member 10b8795Colon CancerHT-29----We examined whether nimbolide can modulate the expression of these proteins. Our results revealed that nimbolide down-regulated the expression of Bcl-2,Bcl-xL, cIAP-1, cIAP-2, I-FLICE, survivin, and XIAP. The effect of nimbolide was most prominent at 5 and 10 M. Overall, these results suggest that nimbolide can down-regulate cell survival protein.21078664
TP53tumor protein p537157Cervix AdenocarcinomaHeLa----To investigate the effects of azadirachtin (135 µ M)and nimbolide (5 µ M) on the expression of markers associated with cell survival, proliferation and apoptosis, we analysed the mRNA and protein expression. Quantifi cation of each band by densitometric analysis revealed signifi cant increase in the expression of p53,p21, I k B, Bid, Bad, Bax, cytosolic cytochrome C,Apaf-1, caspase -2L, -6, -8, -9 and -3 and PARP cleavage.20429769

 






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