NPACT- Naturally occuring Plant based Anticancerous Compound-Activity-Target DataBase

Compound: Kaempferol

TargetsGene NameGene IDCancerCell LinesIC50ED50EC50GI50RemarkReferences
BAXBCL2-associated X protein581Gastric CancerAGS----Western blot results showed that expression level of Bax was increased in a dose-dependent manner.22194772
BCL2L1BCL2-like 1598Gastric CancerAGS----Western blot results showed that expression level of Bcl-xL was decreased in a dose-dependent manner.22194772
CASP3caspase 3, apoptosis-related cysteine peptidase836Gastric CancerAGS----Flavonoids caused downregulation of procaspase-3 which consequently increased the activity of caspase-3.22194772
CCNB1cyclin B1891Gastric CancerAGS----It reduces the protein level of cyclin B1 in a dose-dependent manner.Decrease of cyclin B1 expression might be a molecular mechanism through which flavonoids induce G2/M arrest.22194772
CDC25Ccell division cycle 25 homolog C (S. pombe)995Gastric CancerAGS----It reduces the protein level of cdc 25c in a dose-dependent manner.Decrease of cdc 25c expression might be a molecular mechanism through which flavonoids induce G2/M arrest.22194772
CDK1cyclin-dependent kinase 1983Gastric CancerAGS----It reduces the protein level of cdc 2 in a dose-dependent manner.Decrease of cdc 2 expression might be a molecular mechanism through which flavonoids induce G2/M arrest.22194772
CDK1cyclin-dependent kinase 1983Human MelanomaOCM-1----Kaempferol,inhibited CDK-1 by 50-70%.11322924
CDKN1Bcyclin-dependent kinase inhibitor 1B (p27, Kip1)1027Human MelanomaOCM-1----The flavone apigenin which arrest cells in G2/M but were without effect on CDK2 activity, affected only marginally the level of p27KIP1 and did not induce p21CIP.11322924
PARP1poly (ADP-ribose) polymerase 1142Gastric CancerAGS----During apoptosis, caspases induce PARP cleavage and inactivation and cleavage of PARP helps cellular disassembly and is a marker of cells undergoing apoptosis.22194772
RPS6KA3ribosomal protein S6 kinase, 90kDa, polypeptide 36197Colon cancerHCT-116----NTD RSK2 is a critical kinase domain for substrate phosphorylation, which requires ERK-mediated activation. Kaempferol binds and suppresses theactivation of NTD RSK and that Val82 and Lys100 are critical amino acids for NTD RSK2 activity and inhibition by kaempferol resulted in decreased cell proliferation. targeting RSK2 with natural compounds, such as kaempferol, might be a good strategy for chemopreventive or chemotherapeutic application.19435896
RPS6KA3ribosomal protein S6 kinase, 90kDa, polypeptide 36197Epidermoid carcinoma A-431----NTD RSK2 is a critical kinase domain for substrate phosphorylation, which requires ERK-mediated activation. Kaempferol binds and suppresses theactivation of NTD RSK and that Val82 and Lys100 are critical amino acids for NTD RSK2 activity and inhibition by kaempferol resulted in decreased cell proliferation. targeting RSK2 with natural compounds, such as kaempferol, might be a good strategy for chemopreventive or chemotherapeutic application.19435896
RPS6KA3ribosomal protein S6 kinase, 90kDa, polypeptide 36197MelanomaSK-MEL-28----NTD RSK2 is a critical kinase domain for substrate phosphorylation, which requires ERK-mediated activation. Kaempferol binds and suppresses theactivation of NTD RSK and that Val82 and Lys100 are critical amino acids for NTD RSK2 activity and inhibition by kaempferol resulted in decreased cell proliferation. targeting RSK2 with natural compounds, such as kaempferol, might be a good strategy for chemopreventive or chemotherapeutic application.19435896
RPS6KA3ribosomal protein S6 kinase, 90kDa, polypeptide 36197MelanomaSK-MEL-5----NTD RSK2 is a critical kinase domain for substrate phosphorylation, which requires ERK-mediated activation. Kaempferol binds and suppresses theactivation of NTD RSK and that Val82 and Lys100 are critical amino acids for NTD RSK2 activity and inhibition by kaempferol resulted in decreased cell proliferation. targeting RSK2 with natural compounds, such as kaempferol, might be a good strategy for chemopreventive or chemotherapeutic application.19435896
TNFtumor necrosis factor7124TNF sensitive murine fibroblast cellsL929-20±6 µM--The effects of flavonoid on tumor necrosis factor-R(TNF)-induced cytotoxicity in murine fibroblast L-929 cells was studied. The magnitude of protection and potentiation by this flavonoid was concentration-dependent and these effects were not altered when the flavonoid was added as much as 2 h after TNF treatment.9287415
TNFtumor necrosis factor7124TNF sensitive murine fibroblast cellsL929-25±7 µM--The effects of flavonoid on tumor necrosis factor-R(TNF)-induced cytotoxicity in murine fibroblast L-929 cells was studied. The magnitude of protection and potentiation by this flavonoid was concentration-dependent and these effects were not altered when the flavonoid was added as much as 2 h after TNF treatment.9287415

 






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