NPACT- Naturally occuring Plant based Anticancerous Compound-Activity-Target DataBase

Compound: Genistein

TargetsGene NameGene IDCancerCell LinesIC50ED50EC50GI50RemarkReferences
BAXBCL2-associated X protein581Gastric adenocarcinomaSG7901----Genistein 0.5, 1 and 1.5 mg/kg significantly inhibited carcinoma growth when it was injected near the tumor by 10.8%, 29.9% and 39.6%, respectively. The apoptosis index was increased progressively with increasing genistein dose (28.9% ± 1.2%, 33.8% ± 1.6% and 37.7% ± 1.2%) . The positive rate of Bcl-2 protein was decreased progressively (11.9% ± 0.9%, 5.9% ± 0.7% and 4.2% ± 0.6%) by immunohistochemical staining, with increasing dose of genistein. The density of Bcl-2 mRNA decreased progressively with elongation of time by RT-PCR.18203299
BCL2B-cell CLL/lymphoma 2596Gastric adenocarcinomaSG7901----Genistein 0.5, 1 and 1.5 mg/kg significantly inhibited carcinoma growth when it was injected near the tumor by 10.8%, 29.9% and 39.6%, respectively. The apoptosis index was increased progressively with increasing genistein dose (28.9% ± 1.2%, 33.8% ± 1.6% and 37.7% ± 1.2%) . The positive rate of bax protein was increased progressively (0.9% ± 1.7%, 24.9% ± 0.8% and 29.6% ± 1.7%) by by immunohistochemical staining, with increasing dose of genistein. The density of bax mRNA increased progressively with elongation of time by RT-PCR.18203299
CASP3caspase 3, apoptosis-related cysteine peptidase836Liver CancerHUH7----Compound showed at least a 50%increase in caspase activity showing the ability of these flavonoids to induce apoptosis in HuH-7 cells21495101
CASP3caspase 3, apoptosis-related cysteine peptidase836Breast CarcinomaHs578T----It induced cell apoptosis through a caspase-3-dependent pathway and mediated cell cycle arrest in the G2/M phase. The induction of apoptosis in the breast cancer cell lines by genistein isoflavone confirmed by detection of increased caspase-3 activity in the isoflavone-treated HS578T and MDA-MB-231 cells.19013426
CASP3caspase 3, apoptosis-related cysteine peptidase836Breast CarcinomaMDA-MB-231----It induced cell apoptosis through a caspase-3-dependent pathway and mediated cell cycle arrest in the G2/M phase. The induction of apoptosis in the breast cancer cell lines by genistein isoflavone confirmed by detection of increased caspase-3 activity in the isoflavone-treated HS578T and MDA-MB-231 cells.19013426
CDK1cyclin-dependent kinase 1983Bladder CancerJ82----A dose-dependent inhibition of the cdc2 kinase activity, corresponding to the observed cell cycle arrest effect, was observed in most bladder cancer cell line10656454
CDK1cyclin-dependent kinase 1983Bladder CancerT24----A dose-dependent inhibition of the cdc2 kinase activity, corresponding to the observed cell cycle arrest effect, was observed in most bladder cancer cell line10656454
CDK1cyclin-dependent kinase 1983UroepithelialsHT-1376----A dose-dependent inhibition of the cdc2 kinase activity, corresponding to the observed cell cycle arrest effect, was observed in most bladder cancer cell line10656454
CDK1cyclin-dependent kinase 1983Human MelanomaOCM-1----Genistein inhibited CDK-1 by 50-70.11322924
CDKN1Acyclin-dependent kinase inhibitor 1A (p21, Cip1)1026Human MelanomaOCM-1----Genistein which caused a G2/M block and did not affect CDK2 activity, led to a clear increase in the content of p21CIP.11322924
EGFRepidermal growth factor receptor1956Breast adenocarcinomaMCF-70.7 µg/mL---Genistein is a potent inhibitor of the PTK activity of the EGF-R in vitro. It has been reported to inhibit the growth of a variety of cell lines in vitro stimulated by serum, E2, and growth factors, but a systematic study of the effects of genistein on breast cancer cells has not been reported.8891338
NFKB1nuclear factor of kappa light polypeptide gene enhancer in B-cells 14790Pancreatic cancerCOLO 357----In vivo results were similar to in vitro findings, suggesting that the inactivation of NF-kappaB is at least one of the molecular mechanisms by which genistein potentiates gemcitabine-induced antitumor activity in experimental animal model16204081

 






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