NPACT- Naturally occuring Plant based Anticancerous Compound-Activity-Target DataBase

Compound: 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone

TargetsGene NameGene IDCancerCell LinesIC50ED50EC50GI50RemarkReferences
AKT1v-akt murine thymoma viral oncogene homolog 1207Breast CarcinomaMDA-MB-453----After treatment of 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone for 1 h, immunoblot analysis revealed the phosphorylation of AKT had dramatic decrease. And results showed that phosphorylation of AKT time-dependently decreased in MDA-MB-453 cells following exposed to 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone.19242112
AKT1v-akt murine thymoma viral oncogene homolog 1207Colon CarcinomaHDMEC----This suggested that ON-III has potent inhibitory effect on activation of Akt in a dose-dependent manner in VEGF-stimulating HDMEC cells after treatment with ON-III for 30 min.15703376
AKT1v-akt murine thymoma viral oncogene homolog 1207Human Carcinoma xenograftsGLC-82----This suggested that ON-III has potent inhibitory effect on activation of Akt in a dose-dependent manner in VEGF-stimulating HDMEC cells after treatment with ON-III for 30 min.15703376
AKT1v-akt murine thymoma viral oncogene homolog 1207Liver CarcinomaBEL-7402----This suggested that ON-III has potent inhibitory effect on activation of Akt in a dose-dependent manner in VEGF-stimulating HDMEC cells after treatment with ON-III for 30 min.15703376
BCL2B-cell CLL/lymphoma 2596LeukemiaK-562----The results illustrated that in the same dosage and incubation time, 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone could down-regulate the level of Bcl-2 protein. Resulting in to a lower ratio ofBcl-2/Bax,which might be responsible for the2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone-induced apoptosis in K562 cells.15978939
BCL2B-cell CLL/lymphoma 2596LeukemiaK-562----The results illustrated that in the same dosage and incubation time, 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone could down-regulate the level of Bcl-2 protein. Resulting in to a lower ratio ofBcl-2/Bax,which might be responsible for the2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone-induced apoptosis in K562 cells.15978939
BCL2L11BCL2-like 11 (apoptosis facilitator)10018Breast CarcinomaMDA-MB-453----Treatment with 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone caused an increase in Bim protein levels.The results also revealed that the levels of all three major splicing isoforms—Bim-extra long (EL), Bim-long (L) and Bim-short (S)—were induced after 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone treatment. And Bim upregulation was involved in 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone-induced apoptosis in MDA-MB-453 cells.19242112
ERBB2v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)2064Breast CarcinomaMDA-MB-453----The result of immunoblot analysis using phospho-tyrosine antibody suggested that ON-III inhibited tyrosine kinase phosphorylation of erbB-2 significantly with dose-dependent manner. The expression levels of erbB-2 protein were unaffected following ON-III treatment.19242112
ERBB2v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)2064Breast CarcinomaMDA-MB-453----The result of immunoblot analysis using phospho-tyrosine antibody suggested that ON-III inhibited tyrosine kinase phosphorylation of erbB-2 significantly with dose-dependent manner. The expression levels of erbB-2 protein were unaffected following ON-III treatment.19242112
KDRkinase insert domain receptor (a type III receptor tyrosine kinase)3791Colon CarcinomaHDMEC6.20 µg/mL---The results suggested that 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone (ON-III) significantly inhibited phosphorylation of KDR in a dose-dependent manner at concentrations of 0, 2.5, 5.0, 10.0, and 20.0 g/ml; the IC50 value was 6.20 g/ml inHDMEC cells. But levels of KDR protein were unaffected after ON-III treatment.15703376
KDRkinase insert domain receptor (a type III receptor tyrosine kinase)3791Human Carcinoma xenograftsGLC-826.20 µg/mL---The results suggested that 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone (ON-III) significantly inhibited phosphorylation of KDR in a dose-dependent manner at concentrations of 0, 2.5, 5.0, 10.0, and 20.0 g/ml; the IC50 value was 6.20 g/ml inHDMEC cells. But levels of KDR protein were unaffected after ON-III treatment.15703376
KDRkinase insert domain receptor (a type III receptor tyrosine kinase)3791Liver CarcinomaBEL-74026.20 µg/mL---The results suggested that 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone (ON-III) significantly inhibited phosphorylation of KDR in a dose-dependent manner at concentrations of 0, 2.5, 5.0, 10.0, and 20.0 g/ml; the IC50 value was 6.20 g/ml inHDMEC cells. But levels of KDR protein were unaffected after ON-III treatment.15703376
MAPK1mitogen-activated protein kinase 15594Human Carcinoma xenograftsGLC-82----Results showed that phosphorylation of MAPK time-dependently decreased in MDA-MB-453 cells following exposed to 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone.15703376
MAPK1mitogen-activated protein kinase 15594Liver CarcinomaBEL-7402----Results showed that phosphorylation of MAPK time-dependently decreased in MDA-MB-453 cells following exposed to 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone.15703376
MAPK1mitogen-activated protein kinase 15594Colon CarcinomaHDMEC----This suggested that ON-III has potent inhibitory effect on activation of MAPK in a dose-dependent manner in VEGF-stimulating HDMEC cells after treatment with ON-III for 30 min.15703376
MAPK1mitogen-activated protein kinase 15594Breast CarcinomaMDA-MB-453----This suggested that ON-III has potent inhibitory effect on activation of MAPK in a dose-dependent manner in VEGF-stimulating HDMEC cells after treatment with ON-III for 30 min.19242112
MAPK1mitogen-activated protein kinase 15594Breast CarcinomaMDA-MB-453----This suggested that ON-III has potent inhibitory effect on activation of MAPK in a dose-dependent manner in VEGF-stimulating HDMEC cells after treatment with ON-III for 30 min.19242112

 






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