Welcome to Sequence Card of Peptide
Details of CPPsite entry with Sequence RKKRRQRRR |
| Primary information | |
|---|---|
| PEPTIDE SEQUENCE | RKKRRQRRR |
| CPPsite ID | 2249, 2431, 2610, 2956, 1006, 1329, 1496, |
| PEPTIDE NAME | Tat-PCP, FabRev1-Tat, TAT, Tat(49–57), Tat (49-57), Tat (49-57), Tat (49-57) |
| CHIRALITY | L, L, L, L, L, L, L |
| LINEAR/CYCLIC | Linear, Linear, Linear, Linear, Linear, Linear, Linear |
| SOURCE | Protein derived, Synthetic, Protein derived, Protein derived, Protein derived, Protein derived, Protein derived |
| CATEGORY | Cationic, Cationic, Cationic, Cationic, Cationic, Cationic, Cationic |
| SUB CELLULAR LOCALIZATION | Cytoplasm and Nucleus, Cytoplasm and Nucleus, NA, NA, Probably cytoplasm and nucleus, nucleolus, Nucleus, Unknown |
| N-TERMINAL MODIFICATION | Acetylation, Fused with protein C-terminus, NA, Conjugation with 5(6)-Carboxyfluorescein, Conjugation with fluorescein with 6-aminohexanoic acid (Ahx) spacer, Conjugation with fluorescein, Free |
| C-TERMINAL MODIFICATION | Amidation, Free, NA, Free, Free, Free, Conjugation with GFP |
| CHEMICAL MODIFICATION | ?, L-2-naphthylalanine,miniPEG, 8-amino-3,6-dioxaoctanoic acid, NA, NA, NA, NA, NA, NA |
| UPTAKE EFFICIENCY | Lower efficiency, NA, NA, Low, High, Unknown, Unknown |
| PROPOSED UPTAKE MECHANISM | Endocytic pathway, NA, NA, NA, Probably non-endocytic pathway, Condition dependent, Condition dependent (not determined in this paper) |
| INVITRO/INVIVO | In vitro, In vitro, In vitro, In vivo, In vitro, In vitro, In vitro |
| CELL LINE | HEK293, HeLa, MCF-7, NIH 3T3, A549, Jurkat, H1650, and H1299 cells, PBMC and HEK293T cells, human epithelial lung cell line A549-luc-C8, NA, Jurkat, RAW 264.7, Drosophila S2 cells |
| In VIVO MODEL | NA, NA, NA, Adult zebrafish, NA, NA, NA |
| CARGO | Peptide [PCP, miniPEG-DE(pCAP)LI-NH2], Protein (Rev), Nucleic acid (Calcium comlexed siRNA), Fluorophore (5-Carboxyfluorescein), Fluorophore (fluorescein), Fluorophore (fluorescein), Protein (GFP) |
| PMID | 24896852, 24878961, 21856394, 25894337, 11087855, 12450378, 11211880 |
| PATENT | Unknown, Unknown, Unknown, Unknown, US 6593292, Unknown, US 20090030178 |
| Tertiary Structure (Technique) | 2249 (PEPstrMOD), 2431 (PEPstrMOD), 2610 (PEPstrMOD), 2956 (PEPstrMOD), 1006 (PEPstrMOD), 1329 (PEPstrMOD), 1496 (PEPstrMOD), |