Welcome to Sequence Card of Peptide
Details of CPPsite entry with Sequence GRKKRRQRRRPPQ |
| Primary information | |
|---|---|
| PEPTIDE SEQUENCE | GRKKRRQRRRPPQ |
| CPPsite ID | 2173, 2494, 2589, 2600, 2816, 2830, 2832, 2841, 2897, 1001, 1216, 1748, 1805, |
| PEPTIDE NAME | pTat, pTat, HIV-TAT, CF-Tat.48-60, Tat (48-60), Tat.48-60, Tat, Biotinyl-tat, Tat, Tat (48-60), Tat (48-60), PN280 (SEQ ID NO: 43), Tat |
| CHIRALITY | L, L, L, L, L, L, L, L, L, L, L, L, L |
| LINEAR/CYCLIC | Linear, Linear, Linear, Linear, Linear, Linear, Linear, Linear, Linear, Linear, Linear, Linear, Linear |
| SOURCE | Protein derived, Protein derived, Protein derived, Protein derived, Protein derived, Protein derived, Protein derived, Synthetic, Protein derived, Protein derived, Protein derived, Synthetic, Protein derived |
| CATEGORY | Cationic, Cationic, Cationic, NA, Cationic, Cationic, NA, NA, Cationic, Cationic, Cationic, Unknown, Cationic |
| SUB CELLULAR LOCALIZATION | Vesicles, NA, NA, Cytoplasm and Nucleus, NA, NA, NA, NA, Vesicles, Cytoplasm and nucleus, nucelolus, Unknown, Unknown, Unknown |
| N-TERMINAL MODIFICATION | Conjugation with fluorescein, Conjugation with fluorescein, Free, Conjugation with 5(6)-Carboxyfluorescein, Conjugation with rhodamine B, Free, Free, Free, Conjugation with FITC, Labelled with Cys-acetamidomethyl, Cys addition, Maleimide addition, Conjugation with rhodamine B |
| C-TERMINAL MODIFICATION | Amidation, Amidation, Free, Amidation, Free, Free, Amidation, Amidation, Free, Conjugation with fluorescein maleimide, Free, Amidation, Free |
| CHEMICAL MODIFICATION | NA, NA, NA, NA, NA, Biotinylation, NA, Biotinylation, Linker 6-aminohexamoic acid (Ahx), NA, NA, NA, NA |
| UPTAKE EFFICIENCY | NA, NA, High, NA, NA, NA, NA, NA, High, High, Unknown, Unknown, Less than polyarginine and pAntp but equal to tran |
| PROPOSED UPTAKE MECHANISM | Non-endocytosis, Non endocytosis pathway, NA, Energy dependent process, Endocytic pathway, Endocytic pathway, Direct Translocation, Direct Translocation, Macropinocytosis, Non-endocytic pathway, Energy-dependent endocytic process involving cell membrane lipid rafts, Unknown, Lipid raft-dependent but clathrin-independent endocytic process |
| INVITRO/INVIVO | In vivo, In vitro, In vitro, In vitro, In vitro, In vitro, In vitro, In vitro, In vitro, In vitro, In vitro, In vitro, In vitro |
| CELL LINE | RBL-2H3 cells, CHO-K1, Rat basophilic leukemia RBL-2H3 cells and CHO-K1, Porcine skin, Human glioma T98G cells, U251 cells, PBMCs, GPMVs derived from U87-MG cells, Swiss 3T3 cells and bovine spermatozoa, Swiss 3T3 cells and bovine spermatozoa, HeLa cells, HeLa, HeLa, HCT116, HL-60, K562, Molt4, NIH-3T3 and primary hepatocyte cultures, 9 L/LacZ cells, CHO K1, HeLa, A549 cells |
| In VIVO MODEL | NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA |
| CARGO | Fluorophore (Fluorescein), Fluorophore (Fluorescein), Sodium diclofenac (Na-DFC), Small molecule drug [celecoxib (CLXB)], Fluorophore (Carboxyfluorescein), Fluorophore (Rhodamine B), Fluorophore (FITC), Fluorophore (TAMRA), Fluorophore (TAMRA), Fluorophore (FITC), Fluorophore (fluorescein), Protein (PO and IgG), Nucleic acid (siRNA), Fluorophore (rhodamine) |
| PMID | 25016968, 25016968, 24798650, 21575694, 23707662, 23603097, 23585561, 23585561, 25459448, 9188504, 15859953, 15937518 |
| PATENT | Unknown, Unknown, Unknown, Unknown, Unknown, Unknown, Unknown, Unknown, Unknown, US 20040132970, US 20040132970, US 20060035815, Unknown |
| Tertiary Structure (Technique) | 2173 (PEPstrMOD), 2494 (PEPstrMOD), 2589 (PEPstrMOD), 2600 (PEPstrMOD), 2816 (PEPstrMOD), 2830 (PEPstrMOD), 2832 (PEPstrMOD), 2841 (PEPstrMOD), 2897 (PEPstrMOD), 1001 (PEPstrMOD), 1216 (PEPstrMOD), 1748 (PEPstrMOD), 1805 (PEPstrMOD), |