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Details of SAPdb entry with Sequence KFA
Primary information
SAPdb ID 1067,
PMID23598886
Peptide Name(L-Lys)-(L-Phe)-(L-Ala)
Peptide sequenceKFA
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/ConjuagateConjugate
Conjugate partnerperylene diimide (PDI)
TechniqueTransmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), FTIR (Fourier Transform Infrared) Spectroscopy (Fourier Transform Infrared) Spectroscopy and CD (Circular Dichroism spectroscopy)
SolventTHF(< 10uM)
MethodPeptide is soluble in tetrahydrofuran (THF) it becomes partially soluble. Upon heating a peptide solution at concentration 10x10-4 M to 60 °C for several minutes and then this solution was sonicated (0.40 W cm2 and 40 KHz) for 30–60 min and then kept to 3–5 °C in the refrigerator for 2 days, self-assembled structure formed.
Conc> 1.0x 10-5 M
Temperature3-5 °C
Incubation Time2 days
Year2013
Self assemblyYes
Type of Self assemblyLeft handed Nanohelicals structure
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Nanohelicals
NA
None
N[C@@H](CCCC[NH3])C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C)C=O
Primary information
SAPdb ID 1068,
PMID23598886
Peptide Name(L-Lys)-(L-Phe)-(L-Ala)
Peptide sequenceKFA
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/ConjuagateConjugate
Conjugate partnerperylene diimide (PDI)
TechniqueTransmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), FTIR (Fourier Transform Infrared) Spectroscopy (Fourier Transform Infrared) Spectroscopy and CD (Circular Dichroism spectroscopy)
SolventTHF(< 10uM)
MethodPeptide is soluble in tetrahydrofuran (THF) it becomes partially soluble. Upon heating a peptide solution at concentration 10x10-4 M to 60 °C for several minutes and then cooling to 3–5 °C in the refrigerator for 2 days, self-assembled structure formed.
Conc> 1.0x 10-5 M
Temperature3-5 °C
Incubation Time2 days
Year2013
Self assemblyYes
Type of Self assemblyRight handed Nanohelicals structure
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Nanohelicals
NA
None
N[C@@H](CCCC[NH3])C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C)C=O
Primary information
SAPdb ID 1069,
PMID23598886
Peptide Name(L-Lys)-(L-Phe)-(L-Ala)
Peptide sequenceKFA
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/ConjuagateConjugate
Conjugate partnerperylene diimide (PDI)
TechniqueTransmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), FTIR (Fourier Transform Infrared) Spectroscopy (Fourier Transform Infrared) Spectroscopy and CD (Circular Dichroism spectroscopy)
SolventTHF(< 10uM)
MethodPeptide is soluble in tetrahydrofuran (THF) it becomes partially soluble. Upon heating a peptide solution at concentration 10x10-4 M to 60 °C for several minutes and then cooling to 3–5 °C in the refrigerator for 2 days, self-assembled structure formed.
Conc> 2.0x 10-5 M
Temperature3-5 °C
Incubation Time2 days
Year2013
Self assemblyYes
Type of Self assemblyNanoparticle
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Nanoparticle
5
None
N[C@@H](CCCC[NH3])C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C)C=O
Primary information
SAPdb ID 1120,
PMID21472901
Peptide Name1 or Boc-{(N-Cbz)-Lys}-Phe-Ala
Peptide sequenceKFA
N-Terminal ModificationN-Cbz lysine (Cbz = benzyloxy-carbonyl) and Boc (N-t-Butoxycarbonyl)
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/ConjuagatePeptide derivative
Conjugate partnerCoupling with 5,5’-dibromomethyl-2,2’-bipyridin
TechniqueTransmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM) NMR and Mass spectrometery
SolventTHF (Tetrahydrofuran):Water (1:1)
MethodConjugate 1 was dissolved in THF at a concentration of 4.0mg/mL. Addition of the same amount of water into the prior solution in THF resulted in a transparent solution and the formation of vesicles.
Conc2mg/ml
TemperatureRoom temperature
Year2011
Self assemblyYes
Type of Self assemblyvesicle structure
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
Stable
Nanovesicle
5
None
N.A.
Primary information
SAPdb ID 1121,
PMID21472901
Peptide Name1 or Boc-{(N-Cbz)-Lys}-Phe-Ala
Peptide sequenceKFA
N-Terminal ModificationN-Cbz lysine (Cbz = benzyloxy-carbonyl) and Boc (N-t-Butoxycarbonyl)
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/ConjuagatePeptide derivative
Conjugate partnerCoupling with 5,5’-dibromomethyl-2,2’-bipyridin
TechniqueTransmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM) NMR and Mass spectrometery
SolventTHF (Tetrahydrofuran):Water (1:1)
MethodConjugate 1 was dissolved in THF at a concentration of 4.0mg/mL. Addition of the same amount of water into the prior solution in THF resulted in a transparent solution and the formation of vesicles. Upon ultrasound treatment for about 10 mins, the vesicles transferred into networks of nanofibers
Conc2mg/ml
TemperatureRoom temperature
Incubation Time20-30minutes
Year2011
Self assemblyYes
Type of Self assemblyNanofibril structure
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
Stable
Nanofiber
150
None
N.A.
Primary information
SAPdb ID 1122,
PMID21472901
Peptide Name1 or Boc-{(N-Cbz)-Lys}-Phe-Ala
Peptide sequenceKFA
N-Terminal ModificationN-Cbz lysine (Cbz = benzyloxy-carbonyl) and Boc (N-t-Butoxycarbonyl)
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/ConjuagatePeptide derivative
Conjugate partnerCoupling with 5,5’-dibromomethyl-2,2’-bipyridin
TechniqueTransmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM) NMR and Mass spectrometery
SolventTHF (Tetrahydrofuran):Water (1:1)
MethodConjugate 1 was dissolved in THF at a concentration of 4.0mg/mL. Addition of the same amount of water into the prior solution in THF resulted in a transparent solution and the formation of vesicles. Upon ultrasound treatment for about 10 mins, the vesicles transferred into networks of nanofibers. The nanofibers rearranged into vesicles upon incubation in the 60 C water bath.
Conc2mg/ml
Temperature60°C
Incubation Time20-30minutes
Year2011
Self assemblyYes
Type of Self assemblyvesicle structure
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
Stable
Nanovesicle
NA
None
N.A.