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Details of SAPdb entry with Sequence Beta-AH
Primary information
SAPdb ID 1265,
PMID19904373
Peptide NameN-(4-ntetradecyloxybenzoyl)- L-carnosine
Peptide sequenceBeta-AH
N-Terminal ModificationAcylation (4-n-tetradecyloxybenzoyl)
C-Terminal ModificationFree
Non-Terminal ModificationBeta-A: Beta-alanine
Length2
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueFE - SEM (Field Emission Scanning Electron Microscopy)
Solvent20nM Phosphate buffer
MethodAqeous dispersion of peptide dissolved in phosphate buffer saline (pH7) at concentration 20nM by heating at ~70°C in water and subsequently kept at room temperature to form gel.
Conc20nM
Temperature8
TemperatureRoom temperature
Year2009
Self assemblyYes
Type of Self assemblyHydrogel (consists of interwined ribbons)
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
Stable at Room temperature
Hydrogel
NA
AH
N.A.
Primary information
SAPdb ID 1266,
PMID19904373
Peptide NameN-(4-ntetradecyloxybenzoyl)- L-carnosine
Peptide sequenceBeta-AH
N-Terminal ModificationAcylation
C-Terminal ModificationFree
Non-Terminal ModificationBeta-A: Beta-alanine
Length2
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueFE - SEM (Field Emission Scanning Electron Microscopy)
Solvent20nM Phosphate buffer
MethodAqeous dispersion of peptide dissolved in phosphate buffer saline (pH7) at concentration 20nM by heating at ~70°C in water and subsequently kept at room temperature to form self assembled structure.
Conc20nM
Temperature2
TemperatureRoom temperature
Year2009
Self assemblyYes
Type of Self assemblyBilayer Nanostructures
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Nanoparticle
3.7
AH
N.A.
Primary information
SAPdb ID 1487,
PMID22788380
Peptide NameL-Carnosine (Beta-alanine-L-histidine)
Peptide sequenceBeta-AH
N-Terminal ModificationPalmitic acid
C-Terminal ModificationFree
Non-Terminal ModificationBeta-A: Beta-alanine
Length2
Peptide/ConjuagateConjugate
Conjugate partnerNone
TechniqueCryo - Transmission Electron Microscopy (TEM)
SolventWater
MethodC16-βAH dissolved in Milli-Q water to the desired concentration and mixed using a vortex mixer, followed by sonication in an ultrasonic bath at ∼50 °C for 5−10 min.
Conc1 %wt
Temperature6.5 - 1.5
Temperature50 °C
Incubation Time5 - 10 minutes
Year2012
Self assemblyYes
Type of Self assemblyThick Tapes and thin twisted fibers
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Nanofiber, Nanotape
NA
AH
N.A.
Primary information
SAPdb ID 1488,
PMID22788380
Peptide NameL-Carnosine (Beta-alanine-L-histidine)
Peptide sequenceBeta-AH
N-Terminal ModificationPalmitic acid
C-Terminal ModificationFree
Non-Terminal ModificationBeta-A: Beta-alanine
Length2
Peptide/ConjuagateMixture
Conjugate partnerDPPC
TechniqueCryo - Transmission Electron Microscopy (TEM)
SolventWater
MethodC16-βAH dissolved in Milli-Q water to the desired concentration and mixed using a vortex mixer, followed by sonication in an ultrasonic bath at ∼50 °C for 5−10 min.
Conc0.03 wt % C16-βAH. 1.5 wt % DPPC solutions (
Temperature6.5 - 1.5
Temperature50 °C
Incubation Time5 - 10 minutes
Year2012
Self assemblyYes
Type of Self assemblyThick Tapes and vesicles
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Nanofiber, Nanotape
315
AH
N.A.
Primary information
SAPdb ID 1489,
PMID22788380
Peptide NameL-Carnosine (Beta-alanine-L-histidine)
Peptide sequenceBeta-AH
N-Terminal ModificationPalmitic acid
C-Terminal ModificationFree
Non-Terminal ModificationBeta-A: Beta-alanine
Length2
Peptide/ConjuagateMixture
Conjugate partnerDPPC
TechniqueCryo - Transmission Electron Microscopy (TEM)
SolventWater
MethodA weighted amount of DPPC+C16-βAH was dissolved in ethanol in a 100 mL round-bottom flask. The ethanol was removed to form a thin DPPC film on the walls of the flask under reduced pressure using a rotary evaporator. Any residual solvent was removed under a stream of N2. A weighed amount of water was then added. The flask was then returned to the rotoevaporator, immersed in a water bath, and vigorously rotated for ∼5 min, to reconstitute the lipid film at 50 °C. The solution was thenput in a flask and vortexed at 50 °C for an additional 5 min
Conc1 %wt
Temperature6.5 - 1.5
Temperature50 °C
Incubation Time5 - 10 minutes
Year2012
Self assemblyYes
Type of Self assemblyVesicles
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Nanovesicle
3635
AH
N.A.
Primary information
SAPdb ID 1548,
PMID21338121
Peptide NameL-carnosine
Peptide sequenceBeta-AH
N-Terminal ModificationFmoc [or N-(fluorenyl-9-methoxycarbonyl)]
C-Terminal ModificationFree
Non-Terminal ModificationBeta-A: Beta-alanine
Length2
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueCryo - Transmission Electron Microscopy (TEM)
SolventWater
MethodFmoc- βAH solutions and gels were dissolved in Milli-Q water by sonication in an ultrasonic bath at 35-40 C for 5-10 min. Upon cooling to room temperature, samples were allowed to stand for over three hours. Gelation was verified by tube inversion.
Conc1 wt%
TemperatureRoom temperature
Incubation Time3 hours
Year2011
Self assemblyYes
Type of Self assemblyFibrillar structure
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
Stable upon drying
Nanofiber
15
AH
N.A.
Primary information
SAPdb ID 1912,
PMID28183595
Peptide NameCarnosine (Beta-alanine-L-histidine)
Peptide sequenceBeta-AH
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationBeta-A: Beta-alanine
Length2
Peptide/ConjuagatePeptide
Conjugate partnerNone
Year2016
Self assemblyYes
Type of Self assemblyCarbon Nanotubes
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Nanotube
NA
AH
NA