Details of SAPdb ID 1489 |
Primary information | ||
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SAPdb_ID | 1489 | |
PMID | 22788380 | |
Year | 2012 | |
Name | L-Carnosine (Beta-alanine-L-histidine) | |
Sequence | Beta-AH | |
N-Terminal Modification | Palmitic acid | |
C-Terminal Modification | Free | |
Non-terminal Modication | Beta-A: Beta-alanine | |
Length | 2 | |
Peptide/Conjugate/Mixture | Mixture | |
Conjugate Partner | DPPC | |
Technique | Cryo - Transmission Electron Microscopy (TEM) | |
Solvent | Water | |
Method | A weighted amount of DPPC+C16-Œ≤AH was dissolved in ethanol in a 100 mL round-bottom flask. The ethanol was removed to form a thin DPPC film on the walls of the flask under reduced pressure using a rotary evaporator. Any residual solvent was removed under a stream of N2. A weighed amount of water was then added. The flask was then returned to the rotoevaporator, immersed in a water bath, and vigorously rotated for ‚à º5 min, to reconstitute the lipid film at 50 ¬∞C. The solution was thenput in a flask and vortexed at 50 ¬∞C for an additional 5 min | |
Concentration | 1 %wt | |
pH | 6.5 - 1.5 | |
Temperature | 50 °C | |
Incubation Period | 5 - 10 minutes | |
Self-Assembly Formation | Yes | |
Type of Self-Assembly | Vesicles | |
Size of Self-Assembled structure | ‚à º0.07 to 7.2 Œºm | |
Linear/Cyclic | Linear | |
Stability of Nanostructure | NA | |
Comment | NA | |
Secondary information | ||
Physico-Chemical properties |
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STRUCTURE |
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SMILES | N.A. |