Potential Vaccine Candidates

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This page provides list of potential siRNA that may suppress mRNA sequence of Rhizopus species. These siRNA predicted using software desiRm. Click on desired gene/protein.This page provides list of potential vaccine candidate/epitopes in Rhizopus oryzae proteins that can elicit both arms of immune system; adaptive immune system (memory B-cells and T-cells) and innate immune system (peptide-based vaccine-adjuvants). Here we used following prediction methods, LBtope B-cell epitopes; CTLpred T-cell epitopes; IL-4pred IL-4 inducing epitopes; IL-10pred IL-10 inducing epitopes; IFNepitope IFN-γ inducing epitopes; Propred1 for MHC Class I binders; and Propred for MHC Class II binders. In order to predict peptide-based vaccine adjuvants, we used VaxinPAD.

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S.No.Protein Potential Vaccine CandidateBcell epitope (#1)Tcell epitope (#2)IL-4 inducing epitope (#3)IL-10 inducing epitope (#4)IFN-γinducing epitope (#5)Vaccine Adjuvants (#6)MHC-I (#7)MHC-II (#8)
1Cytochrome P450 14α-sterol demethylase ProteinFMEQKRMIKYesYesYesYesYsYesHLA-B*5401, HLA-B*2705, HLA-A20 Cattle, HLA-A*3101, HLA-A3, HLA-A*1101, HLA-A1DRB1_0305, DRB1_1101
2.1GPI-anchored wall transfer Protein 1RAGLMIMTCYesYesYesYesYesNoHLA-B*5801, HLA-B*5103No
2.2GPI-anchored wall transfer Protein 1LYLGLQDWVYesYesYesYesYesNoHLA-A24, MHC-Kd, HLA-Cw*0401, HLA-B*5103No
2.3GPI-anchored wall transfer Protein 1MTLLGFLRKYesYesYesYesYesNoHLA-A68.1, HLA-A*3101, HLA-A3, HLA-A*1101, HLA-A1No
2.4GPI-anchored wall transfer Protein 1ARFFLTKSVYesYesYesYesYesNoHLA-B*3901, HLA-B*2705, HLA-B*2702, HLA-B14No
2.5GPI-anchored wall transfer Protein 1VMTLLGFLRYesYesYesYesYesNoHLA-A20 Cattle, HLA-A68.1, HLA-A*3302, HLA-A*3101, HLA-A3, HLA-A*1101DRB5_0101, DRB1_0102, DRB5_0105
2.6GPI-anchored wall transfer Protein 1VLYLGLQDWYesYesYesYesYesNoHLA-A3, HLA-B62, HLA-B*2702No
2.7GPI-anchored wall transfer Protein 1VGSFVFSSGYesYesYesYesYesNoNoNo
3High-affinity iron permease ProteinLEQNAWNQVYesYesYesYesYesNoMHC-Kk, HLA-B61, HLA-B60, HLA-B*4403, HLA-B40, HLA-B*3701No
4.1Spore coat ProteinPQLPWPIEKYesYesYesYesYesNoHLA-A3, HLA-A*1101No
4.2Spore coat ProteinLPWPIEKDPYesYesYesYesYesNoNoNo
4.3Spore coat ProteinDRFMRFMVIYesYesYesYesYesYesMHC-Kb, MHC-Dd, HLA-Cw*0301, HLA-B*5201, HLA-B*3901, HLA-B*2705, HLA-B*2702, HLA-B14No
4.4Spore coat ProteinNLPQLPWPIYesYesYesYesYesNoMHC-Dd, MHC-Db, HLA-B62, HLA-A2.1, HLA-A3, HLA-A*0201, HLA-A2, MHC-KkNo
5.1Alkaline Rhizopus protease enzyme ProteinRKRKAEEEIYesYesYesYesYesNoHLA-B*0702, HLA-A20 CattleNo
5.2Alkaline Rhizopus protease enzyme ProteinNPACGFQNFYesYesYesYesYesNoMHC-Ld, HLA-Cw*0702, HLA-Cw*0401, HLA-B*0702, HLA-B62, HLA-B*5801, HLA-B*51, HLA-B*5301, HLA-B*4403, HLA-B*3801, HLA-B*3501No
6ADP-ribosylation factor ProteinSTLKNRQYSYesYesYesYesYesNoMHC-Db, MHC-Db revisedNo
7Dihydrolipoyl dehydrogenase ProteinDAVLVSIGRYesYesYesYesYesNoHLA-A20 Cattle, HLA-A68.1, HLA-A*3302, HLA-A*3101No
8Calcineurin ProteinAFSARGNKRYesYesYesYesYesNoHLA-A*3302, HLA-A*3101No
9.1Serine and Aspartate protease ProteinFWSYLDRQAYesYesYesYesYesNoHLA-B*51, HLA-B*5401, HLA-B*5301No
9.2Serine and Aspartate protease ProteinPQFTHLLLKYesYesYesYesYesNoHLA-B*2705, HLA-A*3101, HLA-A3, HLA-A*1101No
9.3Serine and Aspartate protease ProteinFAELLHCSNYesYesYesYesYesNoHLA-A1, HLA-B*5401, HLA-B*5103No
10.1Chitin deacetylase ProteinVNWPYGAQRYesYesYesYesYesNoHLA-B*2705, HLA-A20 Cattle, HLA-A*3302No
10.2Chitin deacetylase ProteinSYVEMGSNGYesYesYesYesYesNoNoNo
10.3Chitin deacetylase ProteinDSYVEMGSNYesYesYesYesYesNoNoNo
#1:LBtope   #2:CTLpred   #3:IL-4pred   #4:IL-10pred    #5:IFNepitope    #6:VaxinPAD    #7:ProPred-I   #8:ProPred

Vaccine Target Proteins: