This module developed for producing all possible alternate/mutants of a protein. The new peptide is formed by mutating a single residue at a time. It can also provide a prediction score, to identify the peptide can induce IFN-γ region. Users can also sort the prediction score to find the highest scored/best mutant peptide, which can induce IFN-γ production. Further, the user can also generate several mutant peptides of the given sequence. It allows the user to design the best mutant with the highest score and can induce IFN-γ secretion.