Designing Hemolytic Peptides

Systematically evaluate position-scanning single-point mutant analogs to optimize therapeutic therapeutic window and reduce hemolytic liability.

Positional Saturation Scanning Protocol

For an input peptide of length N, the module iterates sequentially from residue Position 1 to Position N. At each position i, the wild-type residue is substituted with each of the other 19 natural amino acids:

• Position 1: X1A, X1C, X1D, ... (19 mutant peptides)
• Position 2: X2A, X2C, X2D, ... (19 mutant peptides)
• ... Position N: XnA, XnC, XnD, ... (19 mutant peptides)

Total Analogs Generated = 19 × N mutant variants + 1 Wild-type baseline.

Full saturation scan. Every residue position is substituted by each of the other 19 amino acids, giving 19 × N variants plus the wild-type lead. Modifications are preserved at every position.

Lead Sequence Submission

Single Peptide Sequence (FASTA or Raw format)

Modified peptides in MAP format are accepted (e.g. {nt:Acet}KWKLFKKIGIGAVLKVLTTG{d}L{ct:Amid}). Modifications are carried into every variant unchanged — only the residue at the scanned position is substituted.

Sequence length: 0 amino acids Will generate: 0 variants (19 × 0)