Upload your PDB file OR paste the structure content directly.
Paste PDB Content:
OR Upload File:
Provide either a file OR pasted content (not both required).
Enter the chain ID of the antigen in your PDB file. Chain IDs are case-sensitive single letters. For multi-chain antigens, use comma-separated IDs.
Chain ID:
Single chain: A Multiple chains: A,B Check SEQRES records in your PDB file to find chain IDs.
Residues with predicted probability ≥ threshold are labeled Interacting. Lower threshold = higher sensitivity. Higher = higher specificity.
Threshold:
Filter deeply buried residues from output. Residues with RSA < 0.05 are almost never epitopes. Recommended for cleaner results.
Minimum RSA: