Welcome to Sequence Card of Peptide
Details of CPPsite entry with Sequence RRRRR |
| Primary information | |
|---|---|
| PEPTIDE SEQUENCE | RRRRR |
| CPPsite ID | 2065, 2345, 2681, 1031, |
| PEPTIDE NAME | R5, Arg5-ELPBC, TMR-R5, R5 |
| CHIRALITY | L, L, L, L |
| LINEAR/CYCLIC | Linear, Linear, Linear, Linear |
| SOURCE | Synthetic, Synthetic, Synthetic, Synthetic |
| CATEGORY | Cationic, Cationic, Cationic, Cationic |
| SUB CELLULAR LOCALIZATION | Cytosol, NA, Cytoplasm, Cytoplasm and nucleus |
| N-TERMINAL MODIFICATION | Free, Conjugation with ELPBC, Conjugation with TMR, Conjugation with fluorescein with 6-aminohexanoic acid (Ahx) spacer |
| C-TERMINAL MODIFICATION | Free, Free, NA, Free |
| CHEMICAL MODIFICATION | NA, NA, NA, NA |
| UPTAKE EFFICIENCY | Ineffective compared to the other polyarginines, Low efficiency at lower temp. but higher at higher temp, NA, Low (relative to Tat (49-57)) |
| PROPOSED UPTAKE MECHANISM | NA, NA, lipid raft dependent macropinocytosis, Probably non-endocytic pathway |
| INVITRO/INVIVO | In vitro, In vitro, In vitro, In vitro |
| CELL LINE | A549, HeLa, U251-MG, HeLa, HEK293 cells, LNZ308, K562 cells, Primary rat fibroblasts cells and U87 cells, Jurkat |
| In VIVO MODEL | NA, NA, NA, NA |
| CARGO | Nucleic acid (pDNA), Peptide (BH3 peptide drug and ELPBC), Fluorophore (TMR), Fluorophore (fluorescein) |
| PMID | 23534410, 24611762, 22465335, 11087855 |
| PATENT | Unknown, Unknown, Unknown, US 6593292 |
| Tertiary Structure (Technique) | 2065 (PEPstrMOD), 2345 (PEPstrMOD), 2681 (PEPstrMOD), 1031 (PEPstrMOD), |