Sequence-based prediction methods only rely on the primary sequence of an antigen without replying on the availability of the 3D structures for the prediction of the linear B-cell epitopes prediction. Previously developed methods predict conformational B-cell epitopes with reasonably high accuracy, but the limitation of these methods is that they require tertiary structure of the antigen. Experimental techniques like X-ray crystallography is used for determining the structure of a protein, is costly, tedious and time consuming. To overcome this limitation several methods has been proposed listed below.