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Details of SAPdb entry with Sequence WW
Primary information
SAPdb ID 1387,
PMID24837840
Peptide NameDitryptophan dipeptide
Peptide sequenceWW
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length2
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueScanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM)
SolventHFIP (1,1,1,3,3,3-hexafluoro-2-Isopropanol) : water (1 : 50 v/v ratio)
Method1 mg of WW was dissolved in 10μL of HFIP followed by addition of 500 μL of water and incubated at 37°C for 24 hours to induce self-assembly
Conc< 1mM
Temperature7.5
Temperature37 °C
Incubation Time24 hours
Year2014
Self assemblyYes
Type of Self assemblyNanosphere
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Nanosphere
230
WW
N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C=O
Primary information
SAPdb ID 1388,
PMID24837840
Peptide NameDitryptophan dipeptide
Peptide sequenceWW
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length2
Peptide/ConjuagatePeptide derivative
Conjugate partnerDN.A. (5'-(CT)5T2-3')
TechniqueScanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM)
SolventHFIP (1,1,1,3,3,3-hexafluoro-2-Isopropanol) : water (1 : 50 v/v ratio)
MethodThe stock solution of WW-(CT)5T2 was prepared by dissolving 4 mg of compound in 1 mL nuclease free water to achieve a 0.05 - 0.5mM WW-(CT)5T2 solution. The sampel was incubated for 1 h in nuclease free
Conc0.05 - 0.5mM
Temperature7.5
Temperature37 °C
Incubation Time24 hours
Year2014
Self assemblyYes
Type of Self assemblyNanosphere
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Nanosphere
230
WW
N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C=O
Primary information
SAPdb ID 1389,
PMID24837840
Peptide NameDitryptophan dipeptide
Peptide sequenceWW
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length2
Peptide/ConjuagatePeptide derivative
Conjugate partnerDN.A. (5'-(CT)5T2-3')
TechniqueScanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM)
SolventHFIP (1,1,1,3,3,3-hexafluoro-2-Isopropanol) : water (1 : 50 v/v ratio)
MethodThe stock solution of WW-(CT)5T2 was prepared by dissolving 4 mg of compound in 1 mL nuclease free water to achieve a 1 mM WW-(CT)5T2 solution. The sampel was incubated for 1 h in nuclease free
Conc>1mM
Temperature7.5
Temperature37 °C
Incubation Time24 hours
Year2014
Self assemblyYes
Type of Self assemblyFibril Nanostructure
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Nanofiber
NA
WW
N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C=O
Primary information
SAPdb ID 1629,
PMID23795243
Peptide NameWW
Peptide sequenceWW
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length2
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueInsilico method: MARTINI coarse - grained molecular dynamic
SolventWater
MethodA simulation for each dipeptide (in their zwitterionic form) was set up using the GROMACS molecular dynamics package. A cubic box with 300 dipeptides, placed randomly with a minimum distance of 3 Å between them, was solvated in standard MARTINI CG water (four water molecules per bead) to a final concentration of ∼0.4 M. A Berendsen thermostat and barostat36 were used to keep the temperature at 303 K and pressure at 1 bar, respectively. Bond lengths in aromatic side chains and the backbone side-chain bonds in I, V, and Y were constrained using the LINCS algorithm.37 All boxes were energy minimized using the steepest descent integrator and then equilibrated for 4 106 time steps of 25 fs. The total screening simulation time equates to 100 ns, but because of the smoothness of the CG potentials, this roughly equates to an effective 400 ns of atomistic simulation time
Conc0.4M
Temperature30 °C
Year2011
Self assemblyYes
Type of Self assemblyAggregate
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Other
NA
WW
N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C=O
Primary information
SAPdb ID 1854,
PMID27548765
Peptide NameWW
Peptide sequenceWW
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length2
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueScanning Electron Microscopy (SEM)
Solvent1,1,1,3,3,3-hexafluoro-2-propanol (HFIP)
Conc100 mg/mL
Year2016
Self assemblyNo
Type of Self assemblyNA
Tertiary Structure
(Technique)		Not Predicted),
NA
NA
NA
NA
NA
WW
N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)O