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Details of SAPdb entry with Sequence E-Delta-F
Primary information
SAPdb ID 1294,
PMID22915849
Peptide NameGlutamic acid-dehydroPhenylalanine (E-Delta-F)
Peptide sequenceE-Delta-F
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationDelta-F or Δphe=alpha-beta-dehydrophenylalanine
Length2
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueTransmission Electron Microscopy (TEM), DLS (Dynamic light - scattering)
SolventMethanol and water (50:50; v/v).
MethodStock solutions were prepared by dissolving 2 mg of peptide in 50 μL of methanol, solution is sonicated to dissolve. Assembly was initiated by addition of 1 mL of aqueous methanol (50% v/v) to the stock. Samples were incubated for 24 hours.
Conc2mg/ml
TemperatureRoom temperature
Incubation Time24 hours
Year2012
Self assemblyNo
Type of Self assemblyNo Nanoparticle formation
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
None
NA
EF
N.A.
Primary information
SAPdb ID 1491,
PMID22833945
Peptide NameGlu-alph, beta-dehydroPhenylalanine
Peptide sequenceE-Delta-F
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationDelta-F or Δphe=alpha-beta-dehydrophenylalanine
Length2
Peptide/ConjuagatePeptide derivative
Conjugate partnerNone
TechniqueTransmission Electron Microscopy (TEM) and DLS (Dynamic Light scattering)
Solvent1,1,1,3,3,3-hexa-fluoro-isopropanol/water.
MethodStock solutions of dipeptides were prepared by dissolving 0.1–1 mg of them in 50 μl of HFIP. Solution heated and sonicated to dissolve the dipeptide. Assembly was initiated by addition of double-distilled water (1 ml) to the stock solution. Samples were aged 24–48 h before experiments.
Conc0.1 - 1mg/ml
TemperatureRoom temperature
Incubation Time24 -48 hours for sample aging and 30 - 60 minutes for self assembly
Year2010
Self assemblyYes
Type of Self assemblyVesicle
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
Proteolytically stable
Nanovesicle
110
EF
N.A.
Primary information
SAPdb ID 1496,
PMID18358051
Peptide NameGlu-Delta-Phe (Glutamate -dehydroPhenylalanine)
Peptide sequenceE-Delta-F
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationDelta-F or Δphe=alpha-beta-dehydrophenylalanine
Length2
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueTransmission Electron Microscopy (TEM) and DLS (Dynamic Light scattering)
Solvent1,1,1,3,3,3-hexa-fluoro-isopropanol + Water
Method1-10 mg of the peptide in a small volume (50 íL) of HFIP and subsequent addition of 1 mL of water at near neutral pH
Conc1-10 mg/mL
Temperature7
Incubation Time24 hours
Year2008
Self assemblyYes
Type of Self assemblySpherical structures
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Nanosphere
110
EF
N.A.