Welcome to Entry Card of Lysosomal Enzymes
Details of RareLSD ID 1048 |
| RareLSD_Id | 1048 |
| ENZYME | Legumain (Asparaginyl Endopeptidase) |
| GENE | LGMN |
| E.C.NUMBER | 3.4.22.34 |
| FAMILY | peptidase C13 |
| CYTOGENETICS | 14q32.12 |
| DISEASE | Schistosomiasis |
| SNP | rs2236264 |
| DEPOSIT | Compounds containing asparaginyl bonds |
| REF | 9821970, 9065484, 25630877 |
| TEMP (in Celsius) | 25 |
| pI | NA |
| pH | 5.5 |
| Catalytic Nucleophile | Cys189 |
| Catalytic Acid/Base | NA |
| Substrate | acetylProThrAsn4-methylcoumarin7-amide + H2O |
| Product | acetylProThrAsn + 7-amino4-methylcoumarin |
| Structure(PDB/Phyre2_ID) | 5LUB |
| kM | 0.122 |
| DRUG | NA |
| SEQ LENGTH | 433 |
| AA SEQ | MVWKVAVFLSVALGIGAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPNGTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPNEDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLDARHLIEKSVRKIVSLLAASEAEVEQLLSERAPLTGHSCYPEALLHFRTHCFNWHSPTYEYALRHLYVLVNLCEKPYPLHRIKLSMDHVCLGHY |
| MODIFICATION | N-linked Glycosylation:Asn91,Asn167,Asn263,Asn272,Ubiquitition: Lys318 |
| INHIBITOR | cystatin E/M,cystatin F,cystatin BmCPI2 (Brugia malayi),clitocypin;macrocypin 1,3;NEM;iodoacetamide,RR11a,MV026630,ZDEVDFMK,zDEVDcmk,ZValAlaAspCH(2)F |
| ACTIVE SITE RESIDUE | His148,Cys189 |
| DISULPHIDE BRIDGES | *378-412,*390-429 |
| PSEUDOGENES | PGOHUM00000261841,PGOHUM00000248419 |
| MISCELLANEOUS | NA |
| PARALOGS | PIGK |
| MECHANISM | Hydrolysis of proteins and small molecule substrates at Asn|Xaa bond |
| INHERITANCE PATTERN | Autosomal Dominant |
| ORGAN AFFECTED | Schistosomiasis (katayama fever) |
| ENZYME LOCATION | NA |
| UniProt ID | Q99538 |