Welcome to Entry Card of Lysosomal Enzymes
Details of RareLSD ID 1033 |
| RareLSD_Id | 1033 |
| ENZYME | Cathepsin V |
| GENE | CTSV |
| E.C.NUMBER | 3.4.22.43 |
| FAMILY | Papain cysteine protease (C1) |
| CYTOGENETICS | 9q22.2 |
| DISEASE | NA |
| SNP | rs1000097893,rs1000293830,rs1000372192,rs1000530718,rs1000698763 |
| DEPOSIT | NA |
| REF | 10029531 , 11027133 , 9727401 |
| TEMP (in Celsius) | 22 |
| pI | NA |
| pH | 5.5 -6 |
| Catalytic Nucleophile | Cys25 |
| Catalytic Acid/Base | H2O |
| Substrate | 2aminobenzoylKLRSIKQN(2,4dinitrophenyl)ethylenediamine + H2O |
| Product | 2aminobenzoylKLR + SIKQN(2,4dinitrophenyl)ethylenediamine |
| Structure(PDB/Phyre2_ID) | 3H6S |
| kM | 0.0006 |
| DRUG | NA |
| SEQ LENGTH | 334 |
| AA SEQ | MNLSLVLAAFCLGIASAVPKFDQNLDTKWYQWKATHRRLYGANEEGWRRAVWEKNMKMIELHNGEYSQGKHGFTMAMNAFGDMTNEEFRQMMGCFRNQKFRKGKVFREPLFLDLPKSVDWRKKGYVTPVKNQKQCGSCWAFSATGALEGQMFRKTGKLVSLSEQNLVDCSRPQGNQGCNGGFMARAFQYVKENGGLDSEESYPYVAVDEICKYRPENSVANDTGFTVVAPGKEKALMKAVATVGPISVAMDAGHSSFQFYKSGIYFEPDCSSKNLDHGVLVVGYGFEGANSNNSKYWLVKNSWGPEWGSNGYVKIAKDKNNHCGIATAASYPNV |
| MODIFICATION | N-linked Glycosylation:Asn221,Asn292 |
| INHIBITOR | cystatin E/M,cathepsin V propeptide,clitocypin,macrocypin 1,3,4,BILDMK diazomethane inhibitor of cysteine peptidases,ZDEVDFMK,AcYVADCMK,vinyl sulfone or other Michael acceptor inhibitors of cysteine peptidases |
| ACTIVE SITE RESIDUE | Gln132,Cys138,His277,Asn301 |
| DISULPHIDE BRIDGES | *135-178,*169-211,*270-323 |
| PSEUDOGENES | NA |
| MISCELLANEOUS | NA |
| PARALOGS | CTSF,CTSO,CTSW,CTSL,CTSK,CTSS,CTSH,CTSC,CTSZ,CTSO |
| MECHANISM | recombinant enzyme hydrolyzes proteins (serum albumin, collagen) and synthetic substrates (ZPheArgNHMec > ZLeuArgNHMec > ZValArgNHMec) |
| INHERITANCE PATTERN | NA |
| ORGAN AFFECTED | NA |
| ENZYME LOCATION | NA |
| UniProt ID | O60911 |