Details of RareLED ID Assay_1034 |
Primary information | |
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GENE | LGMN |
NUMBER OF ASSAY | 10 |
ASSAY IDs | 1153765 647580 647581 1153766 1342999 1142243 1268307 1265055 99723 99724 |
Assay Name | 1. Cytotoxicity against HEK293 cells after 48 hrs by MTS assay,2. Inhibition of mouse recombinant legumain using Cbz-Ala-Ala-Asn-AMC as substrate measured every 30 secs for 2.5 hrs by fluorimetry,3. Inhibition of cathepsin L using Cbz-Phe-Arg-AMC as substrate measured every 30 secs for 2.5 hrs by fluorimetry,4. Cytotoxicity against human M38L cells after 48 hrs by MTS assay,5. Inhibition Assay: Assay buffers consist of 20 mM citric acid, 60 mM disodium hydrogen orthophosphate, 1 mM EDTA, 0.1% CHAPS, 4 mM DTT, pH 5.8 for legumain, 50 mM dihydrogen sodium orthophosphate, 1 mM EDTA, 5 mM DTT, pH 6.25 for cathepsin B and cathepsin Land 100 mM Tris, 0.1% CHAPS, 10% sucrose, 10 mM DTT, pH 7.4 for caspase-3. Concentrations of substrates during the measurement were 10 nM (legumain, cathepsin Land caspase-3) and 50 nM (cathepsin B) and concentration of enzymes were 100 nM for cathepsin Land caspase-3, 270 nM for legumain and 360 nM for cathepsin B. Each enzyme was incubated with inhibitor concentrations ranging from 1 nM to 1 mM in the presence of the substrates.,6. Inhibition of recombinant human legumain using Z-Ala-Ala-Asn-AMC fluorogenic substrate,7. Inhibition of recombinant human legumain using Z-Ala-Ala-Asn-MCA as substrate preincubated with protein followed by substrate addition measured every 2 mins for 60 mins by microplate reader analysis,8. Inhibition of recombinant human legumain using Z-Ala-Ala-Asn-MCA substrate measured every 2 mins for 60 mins by microplate reader analysis,9. Inhibitory concentration was evaluated for Irreversible Inhibition of Schistosoma mansoni Legumain,10. Inhibitory concentration was evaluated for irreversible inhibition of Schistosoma mansoni Legumain |
ACTIVITY | Substance BioActivity: 3 Active, 2 Activity <= 1 µM, 3 Tested Substance BioActivity: 14 Active, 17 Activity <= 1 µM, 17 Tested Substance BioActivity: 17 Tested Substance BioActivity: 3 Active, 3 Activity <= 1 µM, 3 Tested Substance BioActivity: 1 Active, 1 Activity <= 1 µM, 1 Tested Substance BioActivity: 30 Active, 1 Activity <= 1 nM, 29 Activity <= 1 µM, 30 Tested Substance BioActivity: 25 Active, 9 Activity <= 1 µM, 36 Tested Substance BioActivity: 25 Active, 25 Activity <= 1 µM, 29 Tested Substance BioActivity: 3 Active, 3 Activity <= 1 µM, 3 Tested Substance BioActivity: 1 Active, 1 Activity <= 1 µM, 1 Tested |