Welcome to Entry Card of Lysosomal Enzymes


Details of RareLED ID Assay_1031
Primary information
GENEHEXB
NUMBER OF ASSAY11
ASSAY IDs331672 1227114 1227100 748368 1314807 1227112 1227102 1227101 1227099 1227098 748372
Assay Name1. Inhibition of human beta hexosaminidase,2. Inhibition of human placental HexB using MUGS substrate incubated for 1 to 2 hrs at pH 7.0 by spectrofluorometry,3. Inhibition of human placental HexB using MUGS substrate incubated for 1 to 2 hrs at pH 4.5 by spectrofluorometry,4. Competitive inhibition of human beta-N-acetyl-D-hexosaminidase-B using 4-Methylumbelliferyl N-acetyl-beta-D-glucosaminide as substrate assessed as release of 4-Methylumbelliferyl fluorophore measured for 7 mins,5. Inhibition of beta-hexosaminidase alpha/beta in human skin fibroblast lysate using 4-MU-(2-acetamido-2-deoxy)-beta-D-glucopyranoside as substrate assessed as 4-MU liberation by fluorescence assay,6. Inhibition of HexA in normal human fibroblasts using MUGS substrate incubated for 1 to 2 hrs at pH 4.5 by spectrofluorometry,7. Inhibition of human placental HexA using pNPGlcNAc substrate,8. Inhibition of beta-N-acetylhexosaminidase (unknown origin),9. Inhibition of human placental HexA using MUGS substrate incubated for 1 to 2 hrs at pH 4.5 by spectrofluorometry,10. Inhibition of human placental HexA using MUGS substrate incubated for 1 to 2 hrs at pH 7.0 by spectrofluorometry,11. Inhibition of human beta-N-acetyl-D-hexosaminidase-A/B using 4-Methylumbelliferyl N-acetyl-beta-D-glucosaminide as substrate incubated for 10 mins prior to substrate addition by spectrofluorometric analysis
ACTIVITYSubstance BioActivity: 2 Tested Substance BioActivity: 2 Active, 2 Tested Substance BioActivity: 2 Active, 2 Tested Substance BioActivity: 2 Active, 1 Activity <= 1 µM, 2 Tested Substance BioActivity: 2 Tested Substance BioActivity: 2 Active, 2 Tested Substance BioActivity: 9 Active, 17 Tested Substance BioActivity: 1 Active, 1 Tested Substance BioActivity: 2 Active, 2 Tested Substance BioActivity: 2 Active, 2 Tested Substance BioActivity: 19 Active, 3 Activity <= 1 µM, 31 Tested