Welcome to Entry Card of Lysosomal Enzymes

Details of RareLED ID Assay_1026

Primary information
GENE	GLA
NUMBER OF ASSAY	28
ASSAY IDs	488405 1330591 488410 488407 488406 488404 488403 488402 488401 488400 488399 488398 488424 1330592 488411 488408 1330609 2578 488846 389856 240937 73341 37133 37111 37130 219479 37125 1281320
Assay Name	1. Inhibition of human lysosome alpha-galactosidase assessed as p-nitrophenol release by spectrometric analysis,2. Inhibition of human recombinant lysosomal alpha GAL-A using 4-methylberiilyl alpha-D-galactopyranoside as substrate at pH 4.6 after 10 mins by fluorescence assay,3. Inhibition of bovine epididymis alpha-L-fucosidase assessed as p-nitrophenol release at pH 5.5 by spectrometric analysis,4. Inhibition of rat intestinal lactase assessed as p-nitrophenol release by spectrometric analysis,5. Inhibition of bovine liver beta-galactosidase assessed as p-nitrophenol release by spectrometric analysis,6. Inhibition of coffee bean alpha-galactosidase assessed as p-nitrophenol release at pH 6.5 by spectrometric analysis,7. Inhibition of bovine liver beta-glucosidase assessed as p-nitrophenol release at pH 6.8 by spectrometric analysis,8. Inhibition of Caldocellum saccharolyticum beta-glucosidase assessed as p-nitrophenol release at pH 5 by spectrometric analysis,9. Inhibition of almond beta-glucosidase assessed as p-nitrophenol release at pH 5 by spectrometric analysis,10. Inhibition of rat intestinal maltase assessed as D-glucose release at pH 5.8 after 10 to 30 mins,11. Inhibition of rice alpha-glucosidase assessed as D-glucose release at pH 5 after 10 to 30 mins,12. Inhibition of yeast alpha-glucosidase assessed as p-nitrophenol release at pH 6.8 by spectrometric analysis,13. Competitive inhibition of human lysosome alpha-galactosidase by Lineweaver-Burke plot analysis,14. Inhibition of human recombinant lysosomal alpha GAL-A using 4-methylberiilyl alpha-D-galactopyranoside as substrate at pH 7 after 15 mins by fluorescence assay,15. Inhibition of pig kidney trehalase assessed as p-nitrophenol release at pH 6.5 by spectrometric analysis,16. Inhibition of jack bean alpha-mannosidase assessed as p-nitrophenol release at pH 4.5 by spectrometric analysis,17. Cytotoxicity against human lymphocytes assessed as reduction in cell viability after 48 to 72 hrs by alamar blue assay,18. qHTS Assay for Inhibitors and Activators of N370S glucocerebrosidase as a Potential Chaperone Treatment of Gaucher Disease: Alpha-Galactosidase Counterscreen,19. qHTS Assay for Inhibitors and Activators of N370S glucocerebrosidase as a Potential Chaperone Treatment of Gaucher Disease: Alpha-Galactosidase Counterscreen for Probe SAR,20. Activity of BuChE in whole blood,21. Inhibitory concentration against human alpha-galactosidase,22. Inhibitory activity measured against alpha-galactosidase of coffee bean by colorimetric assay using the D-glucose oxidase-peroxidase method,23. Concentration required to inhibit alpha-galactocosidase,24. Compound tested for inhibition of alpha-galactosidase from Aspergillus niger,25. Inhibitory activity against rice Alpha-galactosidase,26. Compound was evaluated for inhibition of alpha-galactosidase from green coffee beans (sigma G 8507).,27. Compound was tested for inhibition of alpha-galactosidase from green coffee beans.,28. Inhibition of human recombinant lysosomal alpha-galactosidase using 2,4-dinitrophenyl-alpha-D-galactopyranoside as substrate preincubated up to 5 mins followed by substrate addition measured up to 10 mins
ACTIVITY	Substance BioActivity: 4 Active, 2 Activity <= 1 µM, 5 Tested Substance BioActivity: 3 Active, 2 Activity <= 1 µM, 4 Tested Substance BioActivity: 1 Tested Substance BioActivity: 1 Active, 2 Tested Substance BioActivity: 4 Tested Substance BioActivity: 4 Active, 2 Activity <= 1 µM, 5 Tested Substance BioActivity: 3 Tested Substance BioActivity: 1 Active, 4 Tested Substance BioActivity: 1 Active, 2 Tested Substance BioActivity: 3 Tested Substance BioActivity: 2 Active, 4 Tested Substance BioActivity: 1 Tested Substance BioActivity: 1 Active, 1 Activity <= 1 µM, 1 Tested Substance BioActivity: 6 Active, 2 Activity <= 1 µM, 6 Tested Substance BioActivity: 1 Tested Substance BioActivity: 1 Tested Substance BioActivity: 3 Tested Substance BioActivity: 70 Tested Substance BioActivity: 6 Tested Substance BioActivity: 1 Tested Substance BioActivity: 2 Active, 1 Activity <= 1 µM, 4 Tested Substance BioActivity: 1 Active, 2 Tested Substance BioActivity: 2 Tested Substance BioActivity: 1 Ac