| Primary information |
|---|
| OV_ID | OV_580 |
| Virus Name | Alphavirus |
| Strain | M1 |
| Virus type | RNA |
| Virus family | Togaviridae |
| Modification in virus genome | None |
| Virus alone | No |
| Virus in combination iwth drug/radiation | Viral strain in combination with H89(10 μM) for 1 hour |
| Immune gene induction in virus genome | No |
| Source of cell line | ATCC |
| Origin of cell line | Human colorectal carcinoma cell line |
| Cell line | HCT-116 |
| Concentration of cell line | 4000 |
| In-vitro toxicity | Very small toxicity towards human immortalized liver cell line L-02 |
| Assay | MTT assay |
| In-vitro virus concentration | 10 PFU/cell |
| In-vitro result | 20% cancer cell viability at 72 hours post-infection |
| Model organism | No |
| In-vivo virus concentration | No |
| In-vivo toxicity | NA |
| In-vivo result | No response |
| Mode of delivery | NA |
| Pathway induction | JNK, mitochondrial apoptotic pathway, Caspase-3/7, Caspase-9 and Caspase-8 (active caspases activates downstream apoptotic pathway) are activated |
| Immunogenic effect | M1 upregulated transcriptional ISGs (329 shown here) were inhibited by H89 |
| Clinical trial | NA |
| PMID | 27374176 |