The purpose of Ar-NHPred server is to predict aromatic-backbone NH interactions in a protein from amino acid sequence. It uses the multiple sequence alignment and secondary structure information for prediction. For further information, please see AR-NHPred documentation. |
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| About submission form |
| Input Sequence |
| The input sequence for prediction is a one-letter code amino acid sequence in plain text format. The name of protein/query is optional. The sequence can be pasted in the text area provided or can be uploaded through a file specified in the form. |
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| The following figure shows the one-letter input sequence in plain text format. |
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| The prediction method uses the PSIPRED program to have the intermediate PSI-BLAST matrices and secondary structure. The whole process is bit time consuming. The following figure shows the output of Ar-NHPred. |
| Output |
| The output consists of input sequence (sequence submitted by the user) where the donor and acceptor residues involved in Ar-HN interaction are marked in blue and red color. The residue predicted both donor and acceptor or in which the NH moiety is interacting with the aromatic ring of the same residue is marked in green color. Below the input sequence, the results are presented in tabular format consisting of type of Ar-HN interaction, donor and acceptor residues and their respective positions. |
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