tRNAmod provides two different level of predicitons: (1) Sequence level prediction and (2) Genome-wide prediction.
1. Sequence level prediction
In the sequence level prediction, tRNAmod requires tRNA sequences in FASTA format and it will predict Uridine-modifications in the given tRNAs.
2. Genone-wide prediction
In the Genome-wide prediction, user can submit whole genome as an input. First tRNAmod will extract tRNA sequence from given genome using tRNAscan-SE software than it will predict Uridine-modifications in each tRNA separately.