PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Page - 1 of 14
Record - 1 of 349 [TOP]

PolysacDB ID	1038
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Rhizobium leguminosarum (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS is composed of three parts: (1) lipid A, (2) core polysaccharide, and (3) O antigen. The lipid A region contains two glucosamine sugar derivatives, each with three fatty acids and phosphate or pyrophosphate attached. The core polysaccharide has KDO and attached to lipid A. The side chain O is small polysaccharide chain extending outward from the core, it has several peculiar sugars and varies in composition between bacterial strains.
BCSDB Structure	1686
Proposed Function	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature	Mixture of suspension of cell envelop.
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 3
Antibody Type Class	N\A
Assay System	Western blotting, spot imunoassays, agglutination tests
Cross Reactivity	This antibody was cross-reactive with different strains of R.leguminosarum like R.biovars viciae, trifolli and phaseoli.
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N\A
Curator ID	AA + AS
Date of Curation	10-01-2010
References	PMC209711

Record - 2 of 349 [TOP]

PolysacDB ID	1040
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Chlamydia spp. (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS is composed of three parts: (1) lipid A, (2) core polysaccharide, and (3) O antigen. The lipid A region contains two glucosamine sugar derivatives, each with three fatty acids and phosphate or pyrophosphate attached. The core polysaccharide has KDO and attached to lipid A. The side chain O is small polysaccharide chain extending outward from the core, it has several peculiar sugars and varies in composition between bacterial strains.
BCSDB Structure	116693
Proposed Function	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature	Formalin killed L2 EBs
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab L21-6
Antibody Type Class	IgG3
Assay System	Immunobloting
Cross Reactivity	This antibody was cross-reactive with Re LPS of S.typhimurium.
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	Monoclonal antibody L21-6 recognizes chlamydal genus specific epitope.
Curator ID	AA + AS
Date of Curation	12-01-2010
References	PMC263518

Record - 3 of 349 [TOP]

PolysacDB ID	1045
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Salmonella typhimurium SR-11 (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS is composed of three parts: (1) lipid A, (2) core polysaccharide, and (3) O antigen. The lipid A region contains two glucosamine sugar derivatives, each with three fatty acids and phosphate or pyrophosphate attached. The core polysaccharide has KDO and attached to lipid A. The side chain O is small polysaccharide chain extending outward from the core, it has several peculiar sugars and varies in composition between bacterial strains.
BCSDB Structure	N/A
Proposed Function	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature	Live S.typhimurium SR cells.
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	ST-1
Antibody Type Class	IgM
Assay System	ELISA Hemaggluttination assay
Cross Reactivity	This antibody was cross-reactive with Salmonella typhimurium LT-2, Salmonella typhimurium derby.
Proposed Epitope	O-polysaccharide
IEDB Epitope	N/A
Proposed Utility	This antibody can be used in serological assays to detect the desired antigen.
Curator ID	AA + AS
Date of Curation	13-01-2010
References	6203984

Record - 4 of 349 [TOP]

PolysacDB ID	1046
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Salmonella typhimurium SR-11 (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS is composed of three parts: (1) lipid A, (2) core polysaccharide, and (3) O antigen. The lipid A region contains two glucosamine sugar derivatives, each with three fatty acids and phosphate or pyrophosphate attached. The core polysaccharide has KDO and attached to lipid A. The side chain O is small polysaccharide chain extending outward from the core, it has several peculiar sugars and varies in composition between bacterial strains.
BCSDB Structure	N/A
Proposed Function	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature	Live S.typhimurium SR cells.
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Polysera
Antibody Type Class	IgG
Assay System	ELISA Hemaggluttination assay
Cross Reactivity	This antibody was cross-reactive with Salmonella typhimurium LT-2, Salmonella typhimurium derby. Salmonella typhimurium dublin, Salmonella enteritidis and Salmonella paratyphi A.
Proposed Epitope	O-polysaccharide
IEDB Epitope	N/A
Proposed Utility	This antibody can be used in serological assays to detect the desired antigen.
Curator ID	AA + AS
Date of Curation	14-01-2010
References	6203984

Record - 5 of 349 [TOP]

PolysacDB ID	1047
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli strain J5 (chemotype Rc) (NCBI Taxonomy) (Drugpedia)
Basic Structure	The core oligosaccharide part of the LPS consists of the following residues : α-D-Glcp(1-->2)α-D-Glcp(1-->2)α-D-Glcp [branched to α-D-GlcpNAc(1-->3)] (1-->3)α-L-D-man-Hepp [branched to α-D-GlcpNAc(1-->7)α-L-D-man-Hepp(1-->7)] and [branched to a phosphate group] (1-->3)α-L-D-man-Hepp [branched to α-Kdop(2-->4)α-Kdop(2-->4)α-Kdop(2-6)Subst] and [branched to a phosphate group](1-->5)
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 7
Antibody Type Class	IgG3
Assay System	ELISA, Limulus assay, invivo protection assay
Cross Reactivity	This antibody was specific to E. coli J5
Proposed Epitope	Terminal glucose residues
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	14-01-2010
References	2455054

Record - 6 of 349 [TOP]

PolysacDB ID	1048
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli strain J5 (chemotype Rc) (NCBI Taxonomy) (Drugpedia)
Basic Structure	The core oligosaccharide part of the LPS consists of the following residues : α-D-Glcp(1-->2)α-D-Glcp(1-->2)α-D-Glcp [branched to α-D-GlcpNAc(1-->3)] (1-->3)α-L-D-man-Hepp [branched to α-D-GlcpNAc(1-->7)α-L-D-man-Hepp(1-->7)] and [branched to a phosphate group] (1-->3)α-L-D-man-Hepp [branched to α-Kdop(2-->4)α-Kdop(2-->4)α-Kdop(2-6)Subst] and [branched to a phosphate group](1-->5)
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 10
Antibody Type Class	IgG3
Assay System	ELISA, Limulus assay, invivo protection assay
Cross Reactivity	This antibody was specific to E. coli J5
Proposed Epitope	Terminal glucose residues
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	15-01-2010
References	2455054

Record - 7 of 349 [TOP]

PolysacDB ID	1049
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli strain J5 (chemotype Rc) (NCBI Taxonomy) (Drugpedia)
Basic Structure	The core oligosaccharide part of the LPS consists of the following residues : α-D-Glcp(1-->2)α-D-Glcp(1-->2)α-D-Glcp [branched to α-D-GlcpNAc(1-->3)] (1-->3)α-L-D-man-Hepp [branched to α-D-GlcpNAc(1-->7)α-L-D-man-Hepp(1-->7)] and [branched to a phosphate group] (1-->3)α-L-D-man-Hepp [branched to α-Kdop(2-->4)α-Kdop(2-->4)α-Kdop(2-6)Subst] and [branched to a phosphate group](1-->5)
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 2
Antibody Type Class	IgG1
Assay System	ELISA, Limulus assay, invivo protection assay
Cross Reactivity	This antibody ross-reacted with Salmonella minnesota strain R5
Proposed Epitope	Terminal glucose residues
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	15-01-2010
References	2455054

Record - 8 of 349 [TOP]

PolysacDB ID	1050
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli strain J5 (chemotype Rc) (NCBI Taxonomy) (Drugpedia)
Basic Structure	The core oligosaccharide part of the LPS consists of the following residues : α-D-Glcp(1-->2)α-D-Glcp(1-->2)α-D-Glcp [branched to α-D-GlcpNAc(1-->3)] (1-->3)α-L-D-man-Hepp [branched to α-D-GlcpNAc(1-->7)α-L-D-man-Hepp(1-->7)] and [branched to a phosphate group] (1-->3)α-L-D-man-Hepp [branched to α-Kdop(2-->4)α-Kdop(2-->4)α-Kdop(2-6)Subst] and [branched to a phosphate group](1-->5)
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 4
Antibody Type Class	IgG2a
Assay System	ELISA, Limulus assay, invivo protection assay
Cross Reactivity	This antibody ross-reacted with Salmonella minnesota strain R5
Proposed Epitope	Terminal glucose residues
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	16-01-2010
References	2455054

Record - 9 of 349 [TOP]

PolysacDB ID	1051
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli strain J5 (chemotype Rc) (NCBI Taxonomy) (Drugpedia)
Basic Structure	The core oligosaccharide part of the LPS consists of the following residues : α-D-Glcp(1-->2)α-D-Glcp(1-->2)α-D-Glcp [branched to α-D-GlcpNAc(1-->3)] (1-->3)α-L-D-man-Hepp [branched to α-D-GlcpNAc(1-->7)α-L-D-man-Hepp(1-->7)] and [branched to a phosphate group] (1-->3)α-L-D-man-Hepp [branched to α-Kdop(2-->4)α-Kdop(2-->4)α-Kdop(2-6)Subst] and [branched to a phosphate group](1-->5)
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 5
Antibody Type Class	IgG1
Assay System	ELISA, Limulus assay, invivo protection assay
Cross Reactivity	This antibody ross-reacted with Salmonella minnesota strain R5
Proposed Epitope	Terminal glucose residues
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	16-01-2010
References	2455054

Record - 10 of 349 [TOP]

PolysacDB ID	1052
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli strain J5 (chemotype Rc) (NCBI Taxonomy) (Drugpedia)
Basic Structure	The core oligosaccharide part of the LPS consists of the following residues : α-D-Glcp(1-->2)α-D-Glcp(1-->2)α-D-Glcp [branched to α-D-GlcpNAc(1-->3)] (1-->3)α-L-D-man-Hepp [branched to α-D-GlcpNAc(1-->7)α-L-D-man-Hepp(1-->7)] and [branched to a phosphate group] (1-->3)α-L-D-man-Hepp [branched to α-Kdop(2-->4)α-Kdop(2-->4)α-Kdop(2-6)Subst] and [branched to a phosphate group](1-->5)
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 3
Antibody Type Class	IgG3
Assay System	ELISA, Limulus assay, invivo protection assay
Cross Reactivity	This antibody ross-reacted with Salmonella minnesota strain R5
Proposed Epitope	Terminal glucose residues
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	16-01-2010
References	2455054

Record - 11 of 349 [TOP]

PolysacDB ID	1053
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli strain J5 (chemotype Rc) (NCBI Taxonomy) (Drugpedia)
Basic Structure	The core oligosaccharide part of the LPS consists of the following residues : α-D-Glcp(1-->2)α-D-Glcp(1-->2)α-D-Glcp [branched to α-D-GlcpNAc(1-->3)] (1-->3)α-L-D-man-Hepp [branched to α-D-GlcpNAc(1-->7)α-L-D-man-Hepp(1-->7)] and [branched to a phosphate group] (1-->3)α-L-D-man-Hepp [branched to α-Kdop(2-->4)α-Kdop(2-->4)α-Kdop(2-6)Subst] and [branched to a phosphate group](1-->5)
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 11
Antibody Type Class	IgG3
Assay System	ELISA, Limulus assay, invivo protection assay
Cross Reactivity	This antibody ross-reacted with Salmonella minnesota strain R5
Proposed Epitope	Terminal glucose residues
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	16-01-2010
References	2455054

Record - 12 of 349 [TOP]

PolysacDB ID	1054
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli strain J5 (chemotype Rc) (NCBI Taxonomy) (Drugpedia)
Basic Structure	The core oligosaccharide part of the LPS consists of the following residues : α-D-Glcp(1-->2)α-D-Glcp(1-->2)α-D-Glcp [branched to α-D-GlcpNAc(1-->3)] (1-->3)α-L-D-man-Hepp [branched to α-D-GlcpNAc(1-->7)α-L-D-man-Hepp(1-->7)] and [branched to a phosphate group] (1-->3)α-L-D-man-Hepp [branched to α-Kdop(2-->4)α-Kdop(2-->4)α-Kdop(2-6)Subst] and [branched to a phosphate group](1-->5)
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 8
Antibody Type Class	IgG3
Assay System	ELISA, Limulus assay, invivo protection assay
Cross Reactivity	This antibody ross-reacted with Salmonella minnesota strain R5
Proposed Epitope	Terminal glucose residues
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	16-01-2010
References	2455054

Record - 13 of 349 [TOP]

PolysacDB ID	1055
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli strain J5 (chemotype Rc) (NCBI Taxonomy) (Drugpedia)
Basic Structure	The core oligosaccharide part of the LPS consists of the following residues : α-D-Glcp(1-->2)α-D-Glcp(1-->2)α-D-Glcp [branched to α-D-GlcpNAc(1-->3)] (1-->3)α-L-D-man-Hepp [branched to α-D-GlcpNAc(1-->7)α-L-D-man-Hepp(1-->7)] and [branched to a phosphate group] (1-->3)α-L-D-man-Hepp [branched to α-Kdop(2-->4)α-Kdop(2-->4)α-Kdop(2-6)Subst] and [branched to a phosphate group](1-->5)
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 9
Antibody Type Class	IgG3
Assay System	ELISA, Limulus assay, invivo protection assay
Cross Reactivity	This antibody ross-reacted with Salmonella minnesota strain R5
Proposed Epitope	Terminal glucose residues
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	16-01-2010
References	2455054

Record - 14 of 349 [TOP]

PolysacDB ID	1056
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli strain J5 (chemotype Rc) (NCBI Taxonomy) (Drugpedia)
Basic Structure	The core oligosaccharide part of the LPS consists of the following residues : α-D-Glcp(1-->2)α-D-Glcp(1-->2)α-D-Glcp [branched to α-D-GlcpNAc(1-->3)] (1-->3)α-L-D-man-Hepp [branched to α-D-GlcpNAc(1-->7)α-L-D-man-Hepp(1-->7)] and [branched to a phosphate group] (1-->3)α-L-D-man-Hepp [branched to α-Kdop(2-->4)α-Kdop(2-->4)α-Kdop(2-6)Subst] and [branched to a phosphate group](1-->5)
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 13
Antibody Type Class	IgG3
Assay System	ELISA, Limulus assay, invivo protection assay
Cross Reactivity	This antibody ross-reacted with Salmonella minnesota strain R5
Proposed Epitope	Terminal glucose residues
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	16-01-2010
References	2455054

Record - 15 of 349 [TOP]

PolysacDB ID	1057
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli O26:H11 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The O-polysaccharide is composed of trisaccharide repeating units having the following structure. -->3)-α-L-Rhap-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure	20653
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 12F5
Antibody Type Class	IgM
Assay System	ELISA, immunoblotting
Cross Reactivity	This antibody cross-reacted with E. coli O4:NM strain and other E. coli O-negative:NM field isolates
Proposed Epitope	O-antigen was proposed to be the epitope
IEDB Epitope	N/A
Proposed Utility	These MAb have a potential use as immunodiagnostic reagents whether used alone or in combination with other phenotypic or genotypic markers
Curator ID	AA + AS
Date of Curation	17-01-2010
References	PMC92269

Record - 16 of 349 [TOP]

PolysacDB ID	1058
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli O111:NM (NCBI Taxonomy) (Drugpedia)
Basic Structure	O-antigen consists of the following residues : -4)α-D-Glcp [branched to α-Colp(1-->6)] and [branched to α-Colp(1-->3)] (1-->4)α-D-Galp(1-->3)β-D-GlcpNAc(1-
BCSDB Structure	20672
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 15C4
Antibody Type Class	IgG3
Assay System	ELISA, immunoblotting
Cross Reactivity	This antibody cross-reacted with 8 Salmonella O35 isolates
Proposed Epitope	O-antigen was proposed to be the epitope
IEDB Epitope	N/A
Proposed Utility	These MAb have a potential use as immunodiagnostic reagents whether used alone or in combination with other phenotypic or genotypic markers
Curator ID	AA + AS
Date of Curation	17-01-2010
References	PMC92269

Record - 17 of 349 [TOP]

PolysacDB ID	1059
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli O157:H7 (NCBI Taxonomy) (Drugpedia)
Basic Structure	-2)α-D-Rhap4NAc(1-->3)α-L-Fucp(1-->4)β-D-Glcp(1-->3)α-D-GalpNAc(1-
BCSDB Structure	3776
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab S5
Antibody Type Class	IgG3
Assay System	ELISA, immunoblotting, Fluorescent-antibody microscopy
Cross Reactivity	This antibody cross-reacted with group N Salmonella strains [(S. landau, S. soerenga, (S. urbana and S. godesberg]
Proposed Epitope	O-antigen was proposed to be the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab provide a valuable tool for the rapid detection of E. coli O157
Curator ID	AA + AS
Date of Curation	17-01-2010
References	PMC229650

Record - 18 of 349 [TOP]

PolysacDB ID	1060
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli O157:H7 (NCBI Taxonomy) (Drugpedia)
Basic Structure	-2)α-D-Rhap4NAc(1-->3)α-L-Fucp(1-->4)β-D-Glcp(1-->3)α-D-GalpNAc(1-
BCSDB Structure	3776
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 12D6
Antibody Type Class	IgG3
Assay System	ELISA, immunoblotting, Fluorescent-antibody microscopy
Cross Reactivity	This antibody cross-reacted with group N Salmonella strains [(S. landau, S. soerenga, (S. urbana and S. godesberg]
Proposed Epitope	O-antigen was proposed to be the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab provide a valuable tool for the rapid detection of E. coli O157
Curator ID	AA + AS
Date of Curation	18-01-2010
References	PMC229650

Record - 19 of 349 [TOP]

PolysacDB ID	1061
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli O157:H7 (NCBI Taxonomy) (Drugpedia)
Basic Structure	-2)α-D-Rhap4NAc(1-->3)α-L-Fucp(1-->4)β-D-Glcp(1-->3)α-D-GalpNAc(1-
BCSDB Structure	3776
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 13B3
Antibody Type Class	IgG3
Assay System	ELISA, immunoblotting, Fluorescent-antibody microscopy
Cross Reactivity	This antibody cross-reacted with group N Salmonella strains [(S. landau, S. soerenga, (S. urbana and S. godesberg]
Proposed Epitope	O-antigen was proposed to be the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab provide a valuable tool for the rapid detection of E. coli O157
Curator ID	AA + AS
Date of Curation	18-01-2010
References	PMC229650

Record - 20 of 349 [TOP]

PolysacDB ID	1062
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli O157:H7 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 2G9
Antibody Type Class	IgM
Assay System	ELISA, immunoblotting, Fluorescent-antibody microscopy
Cross Reactivity	This antibody cross-reacted with group N Salmonella strains [(S. landau, S. soerenga, (S. urbana and S. godesberg]
Proposed Epitope	Core antigen was proposed to be the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab provide a valuable tool for the rapid detection of E. coli O157
Curator ID	AA + AS
Date of Curation	18-01-2010
References	PMC229650

Record - 21 of 349 [TOP]

PolysacDB ID	1063
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli O157:H7 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 3b7
Antibody Type Class	IgM
Assay System	ELISA, immunoblotting, Fluorescent-antibody microscopy
Cross Reactivity	This antibody cross-reacted with group N Salmonella strains [(S. landau, S. soerenga, (S. urbana and S. godesberg]
Proposed Epitope	Core antigen was proposed to be the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab provide a valuable tool for the rapid detection of E. coli O157
Curator ID	AA + AS
Date of Curation	19-01-2010
References	PMC229650

Record - 22 of 349 [TOP]

PolysacDB ID	1064
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli O157:H7 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 29F8
Antibody Type Class	IgM
Assay System	ELISA, immunoblotting, Fluorescent-antibody microscopy
Cross Reactivity	This antibody cross-reacted with group N Salmonella strains [(S. landau, S. soerenga, (S. urbana and S. godesberg]
Proposed Epitope	Core antigen was proposed to be the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab provide a valuable tool for the rapid detection of E. coli O157
Curator ID	AA + AS
Date of Curation	19-01-2010
References	PMC229650

Record - 23 of 349 [TOP]

PolysacDB ID	1065
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli O157:H7 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 42C2
Antibody Type Class	IgG3
Assay System	ELISA, immunoblotting, Fluorescent-antibody microscopy
Cross Reactivity	This antibody cross-reacted with group N Salmonella strains [(S. landau, S. soerenga, (S. urbana and S. godesberg]
Proposed Epitope	Core antigen was proposed to be the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab provide a valuable tool for the rapid detection of E. coli O157
Curator ID	AA + AS
Date of Curation	19-01-2010
References	PMC229650

Record - 24 of 349 [TOP]

PolysacDB ID	1066
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli O157:H7 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 46 E9
Antibody Type Class	IgM
Assay System	ELISA, immunoblotting, Fluorescent-antibody microscopy
Cross Reactivity	This antibody cross-reacted with group N Salmonella strains [(S. landau, S. soerenga, (S. urbana and S. godesberg]
Proposed Epitope	Core antigen was proposed to be the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab provide a valuable tool for the rapid detection of E. coli O157
Curator ID	AA + AS
Date of Curation	20-01-2010
References	PMC229650

Record - 25 of 349 [TOP]

PolysacDB ID	1069
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Escherichia coli C600 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole formalinized E. coli C600 cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 786
Antibody Type Class	IgG3
Assay System	ELISA and immunoblotting
Cross Reactivity	This antibody cross-reacted with LPS core types K-12, R4, and R2 but did not cross-react with Rl LPS and R3 LPS,
Proposed Epitope	The epitope was proposed to consist of trisaccharide sequence Hexp(1->2)-α-D -Glcp(1->3)α-D-Glcp->(Hepp) at the inner region of the outer core, in which the heptose-distal glucose is immunodominant
IEDB Epitope	N/A
Proposed Utility	This Mab may be used for the detection of LPS core structures but also for analysis of the molecular forms resolved on polyacrylamide gels (banding patterns)
Curator ID	AA + AS
Date of Curation	21-01-2010
References	PMC261975

Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020