PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
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PolysacDB ID1448
Carbohydrate NameCapsular polysaccharide [heteroglycan]   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameEnterococcus faecalis type V   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt is composed of rhamnose, glucose, galactose, mannosamine, and glucosamine
BCSDB StructureN/A
Proposed FunctionThese polysaccharides are major virulence factors
Antigenic NatureGlycoconjugates
Carrier NameTetanus toxoid
Conjugate MethodPSs were oxidized with 0.5 ml of 10 mM NaI4 at room temperature for 90 min, dialyzed and lyophilized. The oxidized PS was reacted with 2 mg of tetanus toxoid in PBS with the addition of 10 mg of NaCNBH3
AntibodiesPolysera
Antibody Type ClassN/A
Assay SystemCompetitive inhibition ELISA
Cross ReactivityThis antibody cross-reacted with E. faecalis strain 68114, 324057B and R19001. did not cross-react with E. faecalis type II PS and vancomycin-resistant E. faecium B210860
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation13-07-2010
ReferencesPMC1538600

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PolysacDB ID1449
Carbohydrate NameCapsular polysaccharide [heteroglycan]   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameEnterococci faecium B210860   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt is composed of rhamnose, glucose, galactose, mannosamine, and glucosamine
BCSDB StructureN/A
Proposed FunctionThese polysaccharides are major virulence factors
Antigenic NatureGlycoconjugates
Carrier NameTetanus toxoid
Conjugate MethodPSs were oxidized with 0.5 ml of 10 mM NaI4 at room temperature for 90 min, dialyzed and lyophilized. The oxidized PS was reacted with 2 mg of tetanus toxoid in PBS with the addition of 10 mg of NaCNBH4
AntibodiesPolysera
Antibody Type ClassN/A
Assay SystemCompetitive inhibition ELISA
Cross ReactivityThis antibody was specific to E. faecium B210860 only and did not cross-react with polysaccharides from E. faecalis type II, 68114, and R19001
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation13-07-2010
ReferencesPMC1538600

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