PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

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Record - 1 of 91 [TOP]

PolysacDB ID	1002
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear (1-->3)-linked mannan backbone singly substituted with nonreducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed Function	Antiphagocytic ; an important virulence factor
Antigenic Nature	Glycoconjugates.
Carrier Name	Tetanus toxoid
Conjugate Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 2E9
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody cross-reacted with Serotype A, B and D
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	Human glucurunoxylomannan monoclonal antibodies are essential to address questions regarding the role of glucurunoxylomannan antibodies in protection against cryptococcal infections
Curator ID	AA + AS
Date of Curation	01-01-2010
References	PMC173409

Record - 2 of 91 [TOP]

PolysacDB ID	1005
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Haemophilus influenzae type B (NCBI Taxonomy) (Drugpedia)
Basic Structure	A relatively simple antigen consisting of repeating units of 3-β-D ribose-(1-->1)-D-ribitol-5-phosphate
BCSDB Structure	23423
Proposed Function	An important virulence determinant
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	Protein solutions (25 mg/ml) and Adipic acid dihydrazide [ADH] (3.45 rag/rag protein) were reacted with three different concentrations of EDAC (0 1, 0.3, and 0.6 rag/rag protein) The pH of the reaction mixture was maintained at 4.7 ± 0.2 with 0.1 N HC! The reaction proceeded at room temperature for 3 h and the reaction mixtures were dialyzed at 3-8°C with two changes/d against 6 liter of 0 2 M NaCI. The albumin and polysaccharide derivatives were then dialyzed against two 6-liter changes of deionized water and freeze-dried. The polysaccharide was activated with CNBr. Briefly, a solution of polysaccharide (5.0 mg/ml), equilibrated at 4°C, was rapidly brought to pH 10.5 with 0.1 N NaOH. 100 mg/ml CNBr was added to a final concentration of 0.4 mg/mg polysaccharide, and the pH maintained at 10.5 for 6 rain. Then the reaction mixture was brought to pH 8 5 with 0.5 M NaHCO3, and the CNBr-actlvated polysaccharide added to an equal weight of ADH-protein. The reaction mixture was tumbled gently overnight at 3-8°C and then centrifuged at 16,000 g, 4°C for 20 ram. The supernatant was passed through a CL-4B Sepharose column, 1 5 × 90 cm, that was equihbrated with 0 2 M ammonium acetate. The void-volume fractions were pooled, dialyzed against 0.01 M phosphate-buffered 0.145 M NaCI, pH 7.0, at 3-8°C, and passed through a 045-nm membrane and stored at 3-8°C
Antibodies	Polysera
Antibody Type Class	IgG and IgA
Assay System	Radioimmunoassay
Cross Reactivity	N/A
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	These antibodies exert their protective effect by initiating complement-mediated activities including opsonization and bacterial lysis
Curator ID	AA + AS
Date of Curation	02-01-2010
References	PMC2185954

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PolysacDB ID	1006
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear (1-->3)-linked mannan backbone singly substituted with nonreducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed Function	Antiphagocytic ; an important virulence factor
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 3B6
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody cross-reacted with Serotypes A, B, C and D
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	Human glucurunoxylomannan monoclonal antibodies are essential to address questions regarding the role of glucurunoxylomannan antibodies in protection against cryptococcal infections
Curator ID	AA + AS
Date of Curation	02-01-2010
References	PMC173409

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PolysacDB ID	1012
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Streptococcus Group B (NCBI Taxonomy) (Drugpedia)
Basic Structure	A basic backbone of the following residues: -3-β-D-Galp-(1-->4) -β-D-Glcp[branched to 1-->4-D-Glp-α-D-NeuNACp]-(1-->6)-β-D-GlcNAcp-1-
BCSDB Structure	6237
Proposed Function	Invades the blood stream and multiply. This property of invasiveness is related to the anti-phagocytic properties conferred by its Capsular polysaccharide
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	Type III polysaccharide was activated with cyanogen bromide at pH 10.5 for 6 min at 4°C in a pH stat. Adipic acid dihydrazide [AH] was added in 0.5 M NaHCO3 to a final concentration of 0.25 M, pH 8.5. After tumbling for 18 h at 3 to 8°C, the reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a 4B-CL Sepharose column. The polysaccharide-containing fractions were pooled, dialyzed against sterile pyrogen-free water, and freeze-dried. A solution containing 10 mg each of type III-AH and tetanus toxoid [TT} per ml was brought to pH 5.6 with 0.1 N HCl. 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide was added to a final concentration of 0.05 M, and the pH was maintained at 5.6 with 0.1 N NaOH for 3 h at room temperature. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and was passed through a 4B-CL Sepharose column (5 by 95 cm) equilibrated in 0.2 M NaCl. The void volume fractions were stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Polysera
Antibody Type Class	Mainly IgG, particularly IgG1 and IgG3
Assay System	Double immunodiffusion, capillary precipitation, ELISA
Cross Reactivity	N/A
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	Antibodies elicited by the conjugates had in vitroopsonic activities proposed to be a correlate of protective immunity
Curator ID	AA + AS
Date of Curation	04-01-2010
References	PMC258520

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PolysacDB ID	1031
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A
BCSDB Structure	130362
Proposed Function	The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 18B7
Antibody Type Class	IgG1
Assay System	Invivo protection assay
Cross Reactivity	This antibody cross-reacted with all 4 serotypes- A, B, C and D.
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody opsonized Serotypes A and D and was shown to activate the complement pathway. This antibody is in pre-clinical development for treatment of Cryptococcus neoformans infections
Curator ID	AA + AS
Date of Curation	09-01-2010
References	PMC105619

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PolysacDB ID	1448
Carbohydrate Name	Capsular polysaccharide [heteroglycan] (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Enterococcus faecalis type V (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is composed of rhamnose, glucose, galactose, mannosamine, and glucosamine
BCSDB Structure	N/A
Proposed Function	These polysaccharides are major virulence factors
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	PSs were oxidized with 0.5 ml of 10 mM NaI4 at room temperature for 90 min, dialyzed and lyophilized. The oxidized PS was reacted with 2 mg of tetanus toxoid in PBS with the addition of 10 mg of NaCNBH3
Antibodies	Polysera
Antibody Type Class	N/A
Assay System	Competitive inhibition ELISA
Cross Reactivity	This antibody cross-reacted with E. faecalis strain 68114, 324057B and R19001. did not cross-react with E. faecalis type II PS and vancomycin-resistant E. faecium B210860
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	13-07-2010
References	PMC1538600

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PolysacDB ID	1449
Carbohydrate Name	Capsular polysaccharide [heteroglycan] (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Enterococci faecium B210860 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is composed of rhamnose, glucose, galactose, mannosamine, and glucosamine
BCSDB Structure	N/A
Proposed Function	These polysaccharides are major virulence factors
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	PSs were oxidized with 0.5 ml of 10 mM NaI4 at room temperature for 90 min, dialyzed and lyophilized. The oxidized PS was reacted with 2 mg of tetanus toxoid in PBS with the addition of 10 mg of NaCNBH4
Antibodies	Polysera
Antibody Type Class	N/A
Assay System	Competitive inhibition ELISA
Cross Reactivity	This antibody was specific to E. faecium B210860 only and did not cross-react with polysaccharides from E. faecalis type II, 68114, and R19001
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	13-07-2010
References	PMC1538600

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PolysacDB ID	1496
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans Serotype A [strain 24064] (NCBI Taxonomy) (Drugpedia)
Basic Structure	The major component of C. neoformans capsular polysaccharide is glucurunoxylomannan [GXM] consisting of an unbranched mannose backbone substituted with xylose, glucuronic acid and O-acetyl residues
BCSDB Structure	N/A
Proposed Function	C. neoformans has a large polysaccharide capsule that is a determinant of virulence. The capsular polysaccharide causes immunologic paralysis and inhibits phagocytosis by macrophages. Soluble C. neoformans capsular polysaccharide enhances human immunodeficiency virus [HIV] infection in cultured cells, suggesting a new pathogenic role in AIDS
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	GXM was conjugated to tetanus toxoid using CDAP precipitation
Antibodies	Polysera
Antibody Type Class	IgG [predominantly IgG1]
Assay System	ELISA
Cross Reactivity	This antibody cross-reacted with Serotype D strains
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	04-08-2010
References	1583327

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PolysacDB ID	1578
Carbohydrate Name	N-propionylated group B meningococcal polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	Nil
Antibodies	Polysera
Antibody Type Class	N/A
Assay System	Radioactive binding inhibition assay, ELISA, Bactericidal assay
Cross Reactivity	This antisera cross-reacted with capsular polysaccharide of E. coli K1
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera was bactericidal for Group B meningococci
Curator ID	AA + AS
Date of Curation	12-09-2010
References	2469720

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PolysacDB ID	1579
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 10
Antibody Type Class	IgG1
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab cross-reacted with non-modified acetylaed group B capsular polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease
Curator ID	AA + AS
Date of Curation	13-09-2010
References	9590252

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PolysacDB ID	1580
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 11
Antibody Type Class	IgG2b
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab cross-reacted with non-modified acetylaed group B capsular polysaccharide. This Mab also cross-reacted with polysialic acid on CHP-134 neuroblastoma cells
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease. This Mab induced bactericidal activity with rabbit complement
Curator ID	AA + AS
Date of Curation	13-09-2010
References	9590252

Record - 12 of 91 [TOP]

PolysacDB ID	1581
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 12
Antibody Type Class	IgG2a
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab cross-reacted with non-modified acetylaed group B capsular polysaccharide. This Mab also cross-reacted with polysialic acid on CHP-134 neuroblastoma cells
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease. This Mab induced bactericidal activity with rabbit and human complement
Curator ID	AA + AS
Date of Curation	13-09-2010
References	9590252

Record - 13 of 91 [TOP]

PolysacDB ID	1582
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 13
Antibody Type Class	IgG3
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab cross-reacted with non-modified acetylaed group B capsular polysaccharide. This Mab also cross-reacted with polysialic acid on CHP-134 neuroblastoma cells
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease. This Mab induced bactericidal activity with rabbit and human complement
Curator ID	AA + AS
Date of Curation	14-09-2010
References	9590252

Record - 14 of 91 [TOP]

PolysacDB ID	1583
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 14
Antibody Type Class	IgG2b
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab cross-reacted with non-modified acetylaed group B capsular polysaccharide. This Mab also cross-reacted with polysialic acid on CHP-134 neuroblastoma cells
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease. This Mab induced bactericidal activity with rabbit complement
Curator ID	AA + AS
Date of Curation	14-09-2010
References	9590252

Record - 15 of 91 [TOP]

PolysacDB ID	1584
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 15
Antibody Type Class	IgG2b
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab cross-reacted with non-modified acetylaed group B capsular polysaccharide. This Mab also cross-reacted with polysialic acid on CHP-134 neuroblastoma cells
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease. This Mab induced bactericidal activity with rabbit complement
Curator ID	AA + AS
Date of Curation	14-09-2010
References	9590252

Record - 16 of 91 [TOP]

PolysacDB ID	1585
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 16
Antibody Type Class	IgG2b
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab cross-reacted with non-modified acetylaed group B capsular polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease. This Mab induced bactericidal activity with rabbit complement
Curator ID	AA + AS
Date of Curation	15-09-2010
References	9590252

Record - 17 of 91 [TOP]

PolysacDB ID	1586
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 17
Antibody Type Class	IgM
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab did not cross-react with non-modified acetylaed group B capsular polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease
Curator ID	AA + AS
Date of Curation	16-09-2010
References	9590252

Record - 18 of 91 [TOP]

PolysacDB ID	1587
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 18
Antibody Type Class	IgG2b
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab cross-reacted with non-modified acetylaed group B capsular polysaccharide. This Mab also cross-reacted with polysialic acid on CHP-134 neuroblastoma cells
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease. This Mab induced bactericidal activity with rabbit and human complement
Curator ID	AA + AS
Date of Curation	16-09-2010
References	9590252

Record - 19 of 91 [TOP]

PolysacDB ID	1588
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 19
Antibody Type Class	IgG2a
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab did not cross-react with non-modified acetylaed group B capsular polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease
Curator ID	AA + AS
Date of Curation	16-09-2010
References	9590252

Record - 20 of 91 [TOP]

PolysacDB ID	1589
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 20
Antibody Type Class	IgG2b
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab cross-reacted with non-modified acetylaed group B capsular polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease
Curator ID	AA + AS
Date of Curation	16-09-2010
References	9590252

Record - 21 of 91 [TOP]

PolysacDB ID	1590
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 21
Antibody Type Class	IgG2b
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab cross-reacted with non-modified acetylaed group B capsular polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease
Curator ID	AA + AS
Date of Curation	16-09-2010
References	9590252

Record - 22 of 91 [TOP]

PolysacDB ID	1591
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 22
Antibody Type Class	IgG2b
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab did not cross-react with non-modified acetylaed group B capsular polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease
Curator ID	AA + AS
Date of Curation	17-09-2010
References	9590252

Record - 23 of 91 [TOP]

PolysacDB ID	1592
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 23
Antibody Type Class	IgG2b
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab did not cross-react with non-modified acetylaed group B capsular polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease
Curator ID	AA + AS
Date of Curation	17-09-2010
References	9590252

Record - 24 of 91 [TOP]

PolysacDB ID	1593
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 24
Antibody Type Class	IgG2b
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab did not cross-react with non-modified acetylaed group B capsular polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease
Curator ID	AA + AS
Date of Curation	17-09-2010
References	9590252

Record - 25 of 91 [TOP]

PolysacDB ID	1594
Carbohydrate Name	N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a homopolymer of α(2-->8) sialic acid
BCSDB Structure	N/A
Proposed Function	This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40°C, then 2 days at ambient temperature
Antibodies	Mab Seam 25
Antibody Type Class	IgG2b
Assay System	ELISA, flow cytometry and indirect immunofluorescence
Cross Reactivity	This Mab cross-reacted with non-modified acetylaed group B capsular polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease
Curator ID	AA + AS
Date of Curation	17-09-2010
References	9590252

Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020