|
|
|
|
Home | Search | Advance Search | Browse | Online Submission | Documentation & FAQ | Team | Contact us |
|
| PolysacDB ID | 1995 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Streptococcus pneumoniae serotype 6B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P- |
| BCSDB Structure | 5205 |
| Proposed Function | N/A |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxin mutant (CRM197) |
| Conjugate Method | N/A |
| Antibodies | Mab Hyp6BM17 |
| Antibody Type Class | IgM |
| Assay System | Sandwich ELISA, opsonization assays |
| Cross Reactivity | This Mab cross-reacted with strain 6A |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab had opsonophagocytic activity |
| Curator ID | AA + AS |
| Date of Curation | 13-12-2010 |
| References | PMC97888 |
| PolysacDB ID | 1996 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Streptococcus pneumoniae serotype 6B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P- |
| BCSDB Structure | 5205 |
| Proposed Function | N/A |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxin mutant (CRM197) |
| Conjugate Method | N/A |
| Antibodies | Mab Hyp6BG6 |
| Antibody Type Class | IgG2b |
| Assay System | Sandwich ELISA, opsonization assays |
| Cross Reactivity | This Mab cross-reacted with strain 6A |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab had opsonophagocytic activity |
| Curator ID | AA + AS |
| Date of Curation | 13-12-2010 |
| References | PMC97888 |
| PolysacDB ID | 1997 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Streptococcus pneumoniae serotype 6B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P- |
| BCSDB Structure | 5205 |
| Proposed Function | N/A |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxin mutant (CRM197) |
| Conjugate Method | N/A |
| Antibodies | Mab Hyp6BG7 |
| Antibody Type Class | IgG1 |
| Assay System | Sandwich ELISA, opsonization assays |
| Cross Reactivity | This Mab cross-reacted with strain 6A |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab had opsonophagocytic activity |
| Curator ID | AA + AS |
| Date of Curation | 14-12-2010 |
| References | PMC97888 |
| PolysacDB ID | 1998 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Streptococcus pneumoniae serotype 6B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P- |
| BCSDB Structure | 5205 |
| Proposed Function | N/A |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxin mutant (CRM197) |
| Conjugate Method | N/A |
| Antibodies | Mab Hyp6BG8 |
| Antibody Type Class | IgG1 |
| Assay System | Sandwich ELISA, opsonization assays |
| Cross Reactivity | This Mab cross-reacted with strain 6A |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab had opsonophagocytic activity |
| Curator ID | AA + AS |
| Date of Curation | 14-12-2010 |
| References | PMC97888 |
| PolysacDB ID | 2435 |
| Carbohydrate Name | Trisaccharide part of Streptococcus pneumoniae type 3 polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Streptococcus pneumoniae Type 3 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | N/A |
| BCSDB Structure | N/A |
| Proposed Function | N/A |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxin mutant (CRM197) |
| Conjugate Method | The saccharide fragments described above were conjugated to CRM197 using diethyl squarate as linker. Elongation of the saccharides with diethyl squarate was performed in ethanol/0.1M sodium phosphate (pH 6.9). The reaction products were purified by solid-phase extraction and coupled to protein in 0.1m sodium borate buffer at pH 9.5 |
| Antibodies | Antisera |
| Antibody Type Class | N/A |
| Assay System | Enzyme-linked immunosorbent assay and in-vitro phagocytosis assay |
| Cross Reactivity | N/A |
| Proposed Epitope | β-d-Glcp-(1-->3)-β-d-GlcpA-(1-->4)-β-d-Glcp |
| IEDB Epitope | N/A |
| Proposed Utility | This antisera provided full protection against challenge with pneumococcal type 3 bacteria |
| Curator ID | AA + AS |
| Date of Curation | 13-05-2011 |
| References | PMC308892 |
| PolysacDB ID | 2466 |
| Carbohydrate Name | Pneumococcal vaccine PP (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Streptococcus pneumoniae (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | This vaccine includes the polysaccharides of 6B, 14, 19F, 18C, 23F serotypes |
| BCSDB Structure | N/A |
| Proposed Function | N/A |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxin mutant (CRM197) |
| Conjugate Method | The oligosaccharide-CRM197 conjugates were synthesized by either first hydrolysing the native oligosaccharide [PS] to smaller fragments and then subjecting the fragments to limited periodate oxidation or by oxidizing the native PS and then hydrolyzing it into smaller fragments. The PS-CRM197 conjugates were synthesized by oxidizing native pneumococcal PS with sodium metaperiodate to introduce random aldehyde functional groups. Oxidized OS or PS fragments were coupled to CRM197 in the presence of sodium cyanoborohydride |
| Antibodies | Human Antisera |
| Antibody Type Class | N/A |
| Assay System | ELISA, Opsonophagocytic killing assay |
| Cross Reactivity | The antisera reacted with 6B serotype, showed good opsonophagocytic activity for 19F serotype. This antisera also cross-reacted with 6A serotype but did not react with 19A serotype |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | The findings suggest that some conjugate vaccines may elicit cross-protection against related polysaccharides better than others |
| Curator ID | AA + AS |
| Date of Curation | 26-05-2011 |
| References | 10515817 |
| PolysacDB ID | 2467 |
| Carbohydrate Name | Pneumococcal vaccine OP (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Streptococcus pneumoniae (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | This vaccine includes oligosaccharides of 6B, 14, 19F, 18C, 23F serotypes |
| BCSDB Structure | N/A |
| Proposed Function | N/A |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxin mutant (CRM197) |
| Conjugate Method | The oligosaccharide-CRM197 conjugates were synthesized by either first hydrolysing the native oligosaccharide [PS] to smaller fragments and then subjecting the fragments to limited periodate oxidation or by oxidizing the native PS and then hydrolyzing it into smaller fragments. The PS-CRM197 conjugates were synthesized by oxidizing native pneumococcal PS with sodium metaperiodate to introduce random aldehyde functional groups. Oxidized OS or PS fragments were coupled to CRM197 in the presence of sodium cyanoborohydride |
| Antibodies | Human Antisera |
| Antibody Type Class | N/A |
| Assay System | ELISA, Opsonophagocytic killing assay |
| Cross Reactivity | The antisera reacted with 6B serotype, showed poor opsonophagocytic activity for 19F serotype. This antisera also cross-reacted with 6A serotype but did not react with 19A serotype |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | The findings suggest that some conjugate vaccines may elicit cross-protection against related polysaccharides better than others |
| Curator ID | AA + AS |
| Date of Curation | 26-05-2011 |
| References | 10515817 |
| PolysacDB ID | 2468 |
| Carbohydrate Name | Pneumococcal vaccine MK (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Streptococcus pneumoniae (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | This vaccine includes oligosaccharides of 6B, 14, 19F, 18C, 23F, 4 and 9V serotypes |
| BCSDB Structure | N/A |
| Proposed Function | N/A |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxin mutant (CRM197) |
| Conjugate Method | The oligosaccharide-CRM197 conjugates were synthesized by either first hydrolysing the native oligosaccharide [PS] to smaller fragments and then subjecting the fragments to limited periodate oxidation or by oxidizing the native PS and then hydrolyzing it into smaller fragments. The PS-CRM197 conjugates were synthesized by oxidizing native pneumococcal PS with sodium metaperiodate to introduce random aldehyde functional groups. Oxidized OS or PS fragments were coupled to CRM197 in the presence of sodium cyanoborohydride |
| Antibodies | Human Antisera |
| Antibody Type Class | N/A |
| Assay System | ELISA, Opsonophagocytic killing assay |
| Cross Reactivity | The antisera reacted with 6B serotype, showed good opsonophagocytic activity for 19F serotype. This antisera also cross-reacted with 6A serotype and 19A serotype |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | The findings suggest that some conjugate vaccines may elicit cross-protection against related polysaccharides better than others |
| Curator ID | AA + AS |
| Date of Curation | 27-05-2011 |
| References | 10515817 |
|
Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |