PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 19

Entry No. - 1   [TOP]
PolysacDB ID1971
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameTetanus toxoid
Conjugation MethodThe oligosaccharide was derivatized with adipic acid dihydrazide and coupled to tetanus toxoid
AntibodiesMab 10H8
Antibody type and classIgG1
Assay SystemEnzyme Inhibition Assay, colony blotting, Opsonophagocytosis assay
Cross-reactivityThis Mab reacted with polysaccharides from 11A, 19F and 23F strain but did not recognize type 6A polysaccharide
Proposed epitopesDisaccharide rhamnose-(1-->4)-ribitol phosphate residues seemed to be the part of the epitope
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation08-12-2010
ReferencesPMC95896


Entry No. - 2   [TOP]
PolysacDB ID1972
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameTetanus toxoid
Conjugation MethodThe oligosaccharide was derivatized with adipic acid dihydrazide and coupled to tetanus toxoid
AntibodiesMab 14A2
Antibody type and classIgG1
Assay SystemEnzyme Inhibition Assay, colony blotting, Opsonophagocytosis assay
Cross-reactivityThis Mab reacted with polysaccharides from 11A strain but did not recognize type 6A polysaccharide
Proposed epitopesDisaccharide rhamnose-(1-->4)-ribitol phosphate residues seemed to be the part of the epitope
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation08-12-2010
ReferencesPMC95896


Entry No. - 3   [TOP]
PolysacDB ID1985
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalin killed whole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Hyp6BG2
Antibody type and classIgG
Assay SystemSandwich ELISA, opsonization assays
Cross-reactivityThis Mab was specific to strain 6B and did not cross-react with strain 6A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation11-12-2010
ReferencesPMC97888


Entry No. - 4   [TOP]
PolysacDB ID1986
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalin killed whole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Hyp6BG3
Antibody type and classIgG
Assay SystemSandwich ELISA, opsonization assays
Cross-reactivityThis Mab was specific to strain 6B and did not cross-react with strain 6A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation11-12-2010
ReferencesPMC97888


Entry No. - 5   [TOP]
PolysacDB ID1987
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalin killed whole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Hyp6BG5
Antibody type and classIgG2a
Assay SystemSandwich ELISA, opsonization assays
Cross-reactivityThis Mab was specific to strain 6B and did not cross-react with strain 6A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation11-12-2010
ReferencesPMC97888


Entry No. - 6   [TOP]
PolysacDB ID1988
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalin killed whole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Hyp6BM1
Antibody type and classIgM
Assay SystemSandwich ELISA, opsonization assays
Cross-reactivityThis Mab was specific to strain 6B and did not cross-react with strain 6A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation11-12-2010
ReferencesPMC97888


Entry No. - 7   [TOP]
PolysacDB ID1989
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalin killed whole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Hyp6BM3
Antibody type and classIgM
Assay SystemSandwich ELISA, opsonization assays
Cross-reactivityThis Mab was specific to strain 6B and did not cross-react with strain 6A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation12-12-2010
ReferencesPMC97888


Entry No. - 8   [TOP]
PolysacDB ID1990
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameKeyhole limpet hemocyanin
Conjugation MethodN/A
AntibodiesMab Hyp6BM6
Antibody type and classIgM
Assay SystemSandwich ELISA, opsonization assays
Cross-reactivityThis Mab cross-reacted with strain 6A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab had opsonophagocytic activity
Curator IDAA + AS
Date of Curation12-12-2010
ReferencesPMC97888


Entry No. - 9   [TOP]
PolysacDB ID1991
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameKeyhole limpet hemocyanin
Conjugation MethodN/A
AntibodiesMab Hyp6BM7
Antibody type and classIgM
Assay SystemSandwich ELISA, opsonization assays
Cross-reactivityThis Mab was specific to strain 6B and did not cross-react with strain 6A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation12-12-2010
ReferencesPMC97888


Entry No. - 10   [TOP]
PolysacDB ID1992
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameKeyhole limpet hemocyanin
Conjugation MethodN/A
AntibodiesMab Hyp6BM8
Antibody type and classIgM
Assay SystemSandwich ELISA, opsonization assays
Cross-reactivityThis Mab cross-reacted with strain 6A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab had opsonophagocytic activity
Curator IDAA + AS
Date of Curation13-12-2010
ReferencesPMC97888


Entry No. - 11   [TOP]
PolysacDB ID1993
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameKeyhole limpet hemocyanin
Conjugation MethodN/A
AntibodiesMab Hyp6BM9
Antibody type and classIgM
Assay SystemSandwich ELISA, opsonization assays
Cross-reactivityThis Mab cross-reacted with strain 6A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab had opsonophagocytic activity
Curator IDAA + AS
Date of Curation13-12-2010
ReferencesPMC97888


Entry No. - 12   [TOP]
PolysacDB ID1994
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameKeyhole limpet hemocyanin
Conjugation MethodN/A
AntibodiesMab Hyp6BM10
Antibody type and classIgM
Assay SystemSandwich ELISA, opsonization assays
Cross-reactivityThis Mab cross-reacted with strain 6A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab had opsonophagocytic activity
Curator IDAA + AS
Date of Curation13-12-2010
ReferencesPMC97888


Entry No. - 13   [TOP]
PolysacDB ID1995
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameDiphtheria toxin mutant (CRM197)
Conjugation MethodN/A
AntibodiesMab Hyp6BM17
Antibody type and classIgM
Assay SystemSandwich ELISA, opsonization assays
Cross-reactivityThis Mab cross-reacted with strain 6A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab had opsonophagocytic activity
Curator IDAA + AS
Date of Curation13-12-2010
ReferencesPMC97888


Entry No. - 14   [TOP]
PolysacDB ID1996
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameDiphtheria toxin mutant (CRM197)
Conjugation MethodN/A
AntibodiesMab Hyp6BG6
Antibody type and classIgG2b
Assay SystemSandwich ELISA, opsonization assays
Cross-reactivityThis Mab cross-reacted with strain 6A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab had opsonophagocytic activity
Curator IDAA + AS
Date of Curation13-12-2010
ReferencesPMC97888


Entry No. - 15   [TOP]
PolysacDB ID1997
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameDiphtheria toxin mutant (CRM197)
Conjugation MethodN/A
AntibodiesMab Hyp6BG7
Antibody type and classIgG1
Assay SystemSandwich ELISA, opsonization assays
Cross-reactivityThis Mab cross-reacted with strain 6A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab had opsonophagocytic activity
Curator IDAA + AS
Date of Curation14-12-2010
ReferencesPMC97888


Entry No. - 16   [TOP]
PolysacDB ID1998
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide consists of the following residues : -2)-α-D-Galp-(1-->3)-α-D-Glcp-(1-->3)-α-L-Rhap-(1-->4)-D-Rib-ol-(5-P-
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameDiphtheria toxin mutant (CRM197)
Conjugation MethodN/A
AntibodiesMab Hyp6BG8
Antibody type and classIgG1
Assay SystemSandwich ELISA, opsonization assays
Cross-reactivityThis Mab cross-reacted with strain 6A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab had opsonophagocytic activity
Curator IDAA + AS
Date of Curation14-12-2010
ReferencesPMC97888


Entry No. - 17   [TOP]
PolysacDB ID1999
Carbohydrate NameDisaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe structure of the disaccharide is α-L-Rhap-(1-->4)-D-Rib-ol-[5-->P-->(CH2)3NH2]
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameKeyhole limpet hemocyanin
Conjugation MethodThe saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA, phagocytosis assays and protection experiments
Cross-reactivityThis antisera was specific to capsular polysaccharide of strain 6B and did not cross-react with 6A strains
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera could passively protect mice against a 6B challenge
Curator IDAA + AS
Date of Curation14-12-2010
ReferencesPMC97953


Entry No. - 18   [TOP]
PolysacDB ID2000
Carbohydrate NameTrisaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe structure of the trisaccharide is -D-Rib-ol-(5-->P-->2)-α-D-Galp-(1-->3)-α-D-Glcp-[1-->O(CH2)3NH2]
BCSDB Structure5205
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameKeyhole limpet hemocyanin
Conjugation MethodThe saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA, phagocytosis assays and protection experiments
Cross-reactivityThis antisera was specific to capsular polysaccharide of strain 6B and did not cross-react with 6A strains
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera could passively protect mice against a 6B challenge
Curator IDAA + AS
Date of Curation14-12-2010
ReferencesPMC97953


Entry No. - 19   [TOP]
PolysacDB ID2001
Carbohydrate NameTetrasaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae serotype 6B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe structure of the trisaccharide is α-L-Rhap-(1-->4)-D-Rib-ol-(5-->P-->2)-α-D-Galp-(1-->3)-α-D-Glcp-[1-->O(CH2)3NH2]
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameKeyhole limpet hemocyanin
Conjugation MethodThe saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA, phagocytosis assays and protection experiments
Cross-reactivityThis antisera cross-reacted with capsular polysaccharide of 6A strains
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera could passively protect mice against a 6B and 6A challenge
Curator IDAA + AS
Date of Curation14-12-2010
ReferencesPMC97953


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