PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Home | Search | Advance Search | Browse | Online Submission | Documentation & FAQ | Team | Contact us
Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 5

Entry No. - 1   [TOP]
PolysacDB ID1970
Carbohydrate Name23-valent pneumococcal vaccine   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesCommercial vaccine preparation
Carrier NameNil
Conjugation MethodNil
AntibodiesPolysera
Antibody type and classIgG
Assay SystemELISA
Cross-reactivityThis antisera was specific to pneumococcal polysaccharide and did not cross-react with Haemophilus influenzae type B capsular polysaccharide
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera would promote research on the efficacy of the vaccine in risk groups and will permit in-depth studies of the immunological response in patients
Curator IDAA + AS
Date of Curation08-12-2010
ReferencesPMC170496


Entry No. - 2   [TOP]
PolysacDB ID2412
Carbohydrate NamePhosphorylcholine (PC)   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus pneumoniae   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsAntibodies directed against phosphorylcholine (PC), a major epitope on the cell wall polysaccharide, protect against pneumococcal infections in mice; the role of anti-PC antibodies in humans, however, remains controversial
Antigenic Nature used to produce antibodiesASRNKANDYTTEYSASVKGRFIVS [peptide1]
Carrier NameBovine serum albumin
Conjugation MethodBSA (5 mg) was dissolved in a 0.1 M sodium citrate solution (pH 5.5; 500 ml) to provide a BSA:peptide ratio of 1:25. Glutaraldehyde (0.1%) was added, and the solution was incubated for 1 h at room temperature. The reaction mixture was dialyzed against phosphate-buffered saline (PBS) for 5 days at 4°C
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA, Opsonization and protection assays
Cross-reactivityN/A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis peptide was able to inhibit the binding of anti-PC monoclonal antibodies to PC-BSA
Curator IDAA + AS
Date of Curation03-05-2011
References10992485


Entry No. - 3   [TOP]
PolysacDB ID2466
Carbohydrate NamePneumococcal vaccine PP   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeStreptococcus pneumoniae   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThis vaccine includes the polysaccharides of 6B, 14, 19F, 18C, 23F serotypes
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameDiphtheria toxin mutant (CRM197)
Conjugation MethodThe oligosaccharide-CRM197 conjugates were synthesized by either first hydrolysing the native oligosaccharide [PS] to smaller fragments and then subjecting the fragments to limited periodate oxidation or by oxidizing the native PS and then hydrolyzing it into smaller fragments. The PS-CRM197 conjugates were synthesized by oxidizing native pneumococcal PS with sodium metaperiodate to introduce random aldehyde functional groups. Oxidized OS or PS fragments were coupled to CRM197 in the presence of sodium cyanoborohydride
AntibodiesHuman Antisera
Antibody type and classN/A
Assay SystemELISA, Opsonophagocytic killing assay
Cross-reactivityThe antisera reacted with 6B serotype, showed good opsonophagocytic activity for 19F serotype. This antisera also cross-reacted with 6A serotype but did not react with 19A serotype
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThe findings suggest that some conjugate vaccines may elicit cross-protection against related polysaccharides better than others
Curator IDAA + AS
Date of Curation26-05-2011
References10515817


Entry No. - 4   [TOP]
PolysacDB ID2467
Carbohydrate NamePneumococcal vaccine OP   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeStreptococcus pneumoniae   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThis vaccine includes oligosaccharides of 6B, 14, 19F, 18C, 23F serotypes
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameDiphtheria toxin mutant (CRM197)
Conjugation MethodThe oligosaccharide-CRM197 conjugates were synthesized by either first hydrolysing the native oligosaccharide [PS] to smaller fragments and then subjecting the fragments to limited periodate oxidation or by oxidizing the native PS and then hydrolyzing it into smaller fragments. The PS-CRM197 conjugates were synthesized by oxidizing native pneumococcal PS with sodium metaperiodate to introduce random aldehyde functional groups. Oxidized OS or PS fragments were coupled to CRM197 in the presence of sodium cyanoborohydride
AntibodiesHuman Antisera
Antibody type and classN/A
Assay SystemELISA, Opsonophagocytic killing assay
Cross-reactivityThe antisera reacted with 6B serotype, showed poor opsonophagocytic activity for 19F serotype. This antisera also cross-reacted with 6A serotype but did not react with 19A serotype
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThe findings suggest that some conjugate vaccines may elicit cross-protection against related polysaccharides better than others
Curator IDAA + AS
Date of Curation26-05-2011
References10515817


Entry No. - 5   [TOP]
PolysacDB ID2468
Carbohydrate NamePneumococcal vaccine MK   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeStreptococcus pneumoniae   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThis vaccine includes oligosaccharides of 6B, 14, 19F, 18C, 23F, 4 and 9V serotypes
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameDiphtheria toxin mutant (CRM197)
Conjugation MethodThe oligosaccharide-CRM197 conjugates were synthesized by either first hydrolysing the native oligosaccharide [PS] to smaller fragments and then subjecting the fragments to limited periodate oxidation or by oxidizing the native PS and then hydrolyzing it into smaller fragments. The PS-CRM197 conjugates were synthesized by oxidizing native pneumococcal PS with sodium metaperiodate to introduce random aldehyde functional groups. Oxidized OS or PS fragments were coupled to CRM197 in the presence of sodium cyanoborohydride
AntibodiesHuman Antisera
Antibody type and classN/A
Assay SystemELISA, Opsonophagocytic killing assay
Cross-reactivityThe antisera reacted with 6B serotype, showed good opsonophagocytic activity for 19F serotype. This antisera also cross-reacted with 6A serotype and 19A serotype
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThe findings suggest that some conjugate vaccines may elicit cross-protection against related polysaccharides better than others
Curator IDAA + AS
Date of Curation27-05-2011
References10515817


Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020