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| PolysacDB ID | 2280 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
| BCSDB Structure | 3226 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF B |
| Antibody type and class | IgM |
| Assay System | ELISA |
| Cross-reactivity | The MASF B antibody reacted with all S. flexneri LPSs tested except 4bR, the rough mutant |
| Proposed epitopes | α-L-Rhap-1-->2-α-L-Rha residues seemed to be the part of the epitope |
| IEDB Epitope | 76891 |
| Proposed Utility | This Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria |
| Curator ID | AA + AS |
| Date of Curation | 17-03-2011 |
| References | 2437036 |
| PolysacDB ID | 2281 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
| BCSDB Structure | 3226 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF Y-1 |
| Antibody type and class | IgM |
| Assay System | ELISA |
| Cross-reactivity | MASF Y-1 bound to 4a and Y LPS |
| Proposed epitopes | N/A |
| IEDB Epitope | 76891 |
| Proposed Utility | This Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria |
| Curator ID | AA + AS |
| Date of Curation | 17-03-2011 |
| References | 2437036 |
| PolysacDB ID | 2282 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
| BCSDB Structure | 3226 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF Y-2 |
| Antibody type and class | IgM |
| Assay System | ELISA |
| Cross-reactivity | MASF Y-2 antibodies bound exclusively to Y LPS |
| Proposed epitopes | N/A |
| IEDB Epitope | 76891 |
| Proposed Utility | This Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria |
| Curator ID | AA + AS |
| Date of Curation | 17-03-2011 |
| References | 2437036 |
| PolysacDB ID | 2283 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
| BCSDB Structure | 3226 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF Y-3 |
| Antibody type and class | IgM |
| Assay System | ELISA |
| Cross-reactivity | MASF Y-3 antibodies bound to LPSs of serotypes 2a, 3b, 4b, and Y |
| Proposed epitopes | N/A |
| IEDB Epitope | 76891 |
| Proposed Utility | This Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria |
| Curator ID | AA + AS |
| Date of Curation | 18-03-2011 |
| References | 2437036 |
| PolysacDB ID | 2284 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
| BCSDB Structure | 3226 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF Y-4 |
| Antibody type and class | IgM |
| Assay System | ELISA |
| Cross-reactivity | MASF Y-4 antibodies bound to 2a, 3b, 4a, 4b, 5a, and Y LPSs |
| Proposed epitopes | N/A |
| IEDB Epitope | 76891 |
| Proposed Utility | This Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria |
| Curator ID | AA + AS |
| Date of Curation | 18-03-2011 |
| References | 2437036 |
| PolysacDB ID | 2285 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
| BCSDB Structure | 3226 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF Y-5 |
| Antibody type and class | IgM |
| Assay System | ELISA |
| Cross-reactivity | MASF Y-5 bound to LPSs of serotypes 2a, 3b, 4a, and Y |
| Proposed epitopes | N/A |
| IEDB Epitope | 76891 |
| Proposed Utility | This Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria |
| Curator ID | AA + AS |
| Date of Curation | 18-03-2011 |
| References | 2437036 |
| PolysacDB ID | 2436 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -3)-α-D-GlcpNAc-(1-->3)-β-L-Rhap-(1-->2)-α-L-Rhap-(1-->2)-β-L-Rhap-(1- |
| BCSDB Structure | 113249 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Phenol killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab Y-A1 |
| Antibody type and class | IgM |
| Assay System | ELISA, immunodiffusion |
| Cross-reactivity | This Mab was specific to Y-LPS |
| Proposed epitopes | MASFY-A1 exhibits distinct preference for the repeating oligosaccharide α-L-Rha(1-->2)α-L-Rha(1-->3)α-L-Rha(1-->3)βGlcNac as a dimer |
| IEDB Epitope | 76696 |
| Proposed Utility | This Mab can be used for antigenic characterization of Shigella LPS |
| Curator ID | AA + AS |
| Date of Curation | 13-05-2011 |
| References | 3531329 |
| PolysacDB ID | 2437 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -3)-α-D-GlcpNAc-(1-->3)-β-L-Rhap-(1-->2)-α-L-Rhap-(1-->2)-β-L-Rhap-(1- |
| BCSDB Structure | 113249 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Phenol killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab Y-A2 |
| Antibody type and class | IgM |
| Assay System | ELISA, immunodiffusion |
| Cross-reactivity | This Mab cross-reacted with 4a LPS |
| Proposed epitopes | Mab Y-A2 exhibits distinct preference for the oligosaccharide α-L-Rha(1-->2)α-L-Rha(1-->3)α-L-Rha(1-->3)βGlcNac |
| IEDB Epitope | 76696 |
| Proposed Utility | This Mab can be used for antigenic characterization of Shigella LPS |
| Curator ID | AA + AS |
| Date of Curation | 13-05-2011 |
| References | 3531329 |
| PolysacDB ID | 2438 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -3)-α-D-GlcpNAc-(1-->3)-β-L-Rhap-(1-->2)-α-L-Rhap-(1-->2)-β-L-Rhap-(1- |
| BCSDB Structure | 113249 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Phenol killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab Y-A3 |
| Antibody type and class | IgM |
| Assay System | ELISA, immunodiffusion |
| Cross-reactivity | This Mab cross-reacted with 3b and 4a LPS |
| Proposed epitopes | This Mab reacted with alkali-treated LPS and O-antigen. Mab Y-A3 exhibits distinct preference for the oligosaccharide α-L-Rha(1-->2)α-L-Rha(1-->3)α-L-Rha(1-->3)βGlcNac |
| IEDB Epitope | 76696 |
| Proposed Utility | This Mab can be used for antigenic characterization of Shigella LPS |
| Curator ID | AA + AS |
| Date of Curation | 14-05-2011 |
| References | 3531329 |
| PolysacDB ID | 2439 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -3)-α-D-GlcpNAc-(1-->3)-β-L-Rhap-(1-->2)-α-L-Rhap-(1-->2)-β-L-Rhap-(1- |
| BCSDB Structure | 113249 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Phenol killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab Y-A4 |
| Antibody type and class | IgM |
| Assay System | ELISA, immunodiffusion |
| Cross-reactivity | This Mab was specific to Y-LPS |
| Proposed epitopes | N/A |
| IEDB Epitope | 76696 |
| Proposed Utility | This Mab can be used for antigenic characterization of Shigella LPS |
| Curator ID | AA + AS |
| Date of Curation | 14-05-2011 |
| References | 3531329 |
| PolysacDB ID | 2440 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -3)-α-D-GlcpNAc-(1-->3)-β-L-Rhap-(1-->2)-α-L-Rhap-(1-->2)-β-L-Rhap-(1- |
| BCSDB Structure | 113249 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Phenol killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab Y-A5 |
| Antibody type and class | IgM |
| Assay System | ELISA, immunodiffusion |
| Cross-reactivity | This Mab was specific to Y-LPS |
| Proposed epitopes | This Mab reacted with alkali-treated LPS and O-antigen |
| IEDB Epitope | 76696 |
| Proposed Utility | This Mab can be used for antigenic characterization of Shigella LPS |
| Curator ID | AA + AS |
| Date of Curation | 15-05-2011 |
| References | 3531329 |
| PolysacDB ID | 2441 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -3)-α-D-GlcpNAc-(1-->3)-β-L-Rhap-(1-->2)-α-L-Rhap-(1-->2)-β-L-Rhap-(1- |
| BCSDB Structure | 113249 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Phenol killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab Y-B1 |
| Antibody type and class | IgM |
| Assay System | ELISA, immunodiffusion |
| Cross-reactivity | This Mab was specific to Y-LPS |
| Proposed epitopes | This Mab reacted with alkali-treated LPS and O-antigen |
| IEDB Epitope | 76696 |
| Proposed Utility | This Mab can be used for antigenic characterization of Shigella LPS |
| Curator ID | AA + AS |
| Date of Curation | 15-05-2011 |
| References | 3531329 |
| PolysacDB ID | 2442 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -3)-α-D-GlcpNAc-(1-->3)-β-L-Rhap-(1-->2)-α-L-Rhap-(1-->2)-β-L-Rhap-(1- |
| BCSDB Structure | 113249 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Phenol killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab Y-B2 |
| Antibody type and class | IgG3 |
| Assay System | ELISA, immunodiffusion |
| Cross-reactivity | This Mab cross-reacted with 1a and 4a LPS |
| Proposed epitopes | This Mab reacted with alkali-treated LPS and O-antigen. Mab Y-B2 exhibits distinct preference for the oligosaccharide α-L-Rha(1-->2)α-L-Rha(1-->3)α-L-Rha(1-->3)βGlcNac repeating as a dimer |
| IEDB Epitope | 76696 |
| Proposed Utility | This Mab can be used for antigenic characterization of Shigella LPS |
| Curator ID | AA + AS |
| Date of Curation | 15-05-2011 |
| References | 3531329 |
| PolysacDB ID | 2443 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -3)-α-D-GlcpNAc-(1-->3)-β-L-Rhap-(1-->2)-α-L-Rhap-(1-->2)-β-L-Rhap-(1- |
| BCSDB Structure | 113249 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Phenol killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab Y-B3 |
| Antibody type and class | IgM |
| Assay System | ELISA, immunodiffusion |
| Cross-reactivity | This Mab cross-reacted with 3b and 4a LPS |
| Proposed epitopes | This Mab reacted with alkali-treated LPS |
| IEDB Epitope | 76696 |
| Proposed Utility | This Mab can be used for antigenic characterization of Shigella LPS |
| Curator ID | AA + AS |
| Date of Curation | 15-05-2011 |
| References | 3531329 |
| PolysacDB ID | 2444 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -3)-α-D-GlcpNAc-(1-->3)-β-L-Rhap-(1-->2)-α-L-Rhap-(1-->2)-β-L-Rhap-(1- |
| BCSDB Structure | 113249 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Phenol killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab Y-B4 |
| Antibody type and class | IgG2a |
| Assay System | ELISA, immunodiffusion |
| Cross-reactivity | This Mab was highly cross-reactive and cross-reacted with 1a, 1b, 2a, 3a, 3b, 4a, 4b, 5a, X LPS |
| Proposed epitopes | N/A |
| IEDB Epitope | 76696 |
| Proposed Utility | This Mab can be used for antigenic characterization of Shigella LPS |
| Curator ID | AA + AS |
| Date of Curation | 16-05-2011 |
| References | 3531329 |
| PolysacDB ID | 2445 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -3)-α-D-GlcpNAc-(1-->3)-β-L-Rhap-(1-->2)-α-L-Rhap-(1-->2)-β-L-Rhap-(1- |
| BCSDB Structure | 113249 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Phenol killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab Y-B5 |
| Antibody type and class | IgM |
| Assay System | ELISA, immunodiffusion |
| Cross-reactivity | This Mab was highly cross-reactive and cross-reacted with 1b, 2b, 3a, 4a and X LPS |
| Proposed epitopes | This Mab reacted with alkali-treated LPS |
| IEDB Epitope | 76696 |
| Proposed Utility | This Mab can be used for antigenic characterization of Shigella LPS |
| Curator ID | AA + AS |
| Date of Curation | 16-05-2011 |
| References | 3531329 |
| PolysacDB ID | 2446 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -3)-α-D-GlcpNAc-(1-->3)-β-L-Rhap-(1-->2)-α-L-Rhap-(1-->2)-β-L-Rhap-(1- |
| BCSDB Structure | 113249 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Phenol killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF-1 |
| Antibody type and class | IgG2b |
| Assay System | ELISA, immunodiffusion |
| Cross-reactivity | This Mab was highly cross-reactive and cross-reacted with 4a LPS |
| Proposed epitopes | MASFY-1 exhibits distinct preference for the oligosaccharide α-L-Rha(1-->2)α-L-Rha(1-->3)α-L-Rha(1-->3)βGlcNac repeating as a dimer |
| IEDB Epitope | 76696 |
| Proposed Utility | This Mab can be used for antigenic characterization of Shigella LPS |
| Curator ID | AA + AS |
| Date of Curation | 17-05-2011 |
| References | 3531329 |
| PolysacDB ID | 2447 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -3)-α-D-GlcpNAc-(1-->3)-β-L-Rhap-(1-->2)-α-L-Rhap-(1-->2)-β-L-Rhap-(1- |
| BCSDB Structure | 113249 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Phenol killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF-2 |
| Antibody type and class | IgG1 |
| Assay System | ELISA, immunodiffusion |
| Cross-reactivity | This Mab was specific to Y-LPS |
| Proposed epitopes | MASFY-2 exhibits distinct preference for the oligosaccharide α-L-Rha(1-->2)α-L-Rha(1-->3)α-L-Rha(1-->3)βGlcNac repeating as a dimer |
| IEDB Epitope | 76696 |
| Proposed Utility | This Mab can be used for antigenic characterization of Shigella LPS |
| Curator ID | AA + AS |
| Date of Curation | 17-05-2011 |
| References | 3531329 |
| PolysacDB ID | 2448 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -3)-α-D-GlcpNAc-(1-->3)-β-L-Rhap-(1-->2)-α-L-Rhap-(1-->2)-β-L-Rhap-(1- |
| BCSDB Structure | 113249 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Phenol killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF-3 |
| Antibody type and class | IgG2 |
| Assay System | ELISA, immunodiffusion |
| Cross-reactivity | This Mab was specific to Y-LPS |
| Proposed epitopes | MASFY-3 exhibits distinct preference for the oligosaccharide α-L-Rha(1-->2)α-L-Rha(1-->3)α-L-Rha(1-->3)βGlcNac |
| IEDB Epitope | 76696 |
| Proposed Utility | This Mab can be used for antigenic characterization of Shigella LPS |
| Curator ID | AA + AS |
| Date of Curation | 18-05-2011 |
| References | 3531329 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |