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| PolysacDB ID | 2277 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype 4a (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
| BCSDB Structure | 4951 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF IV-1 |
| Antibody type and class | IgG3 |
| Assay System | ELISA |
| Cross-reactivity | MASF IV-1 antibodies recognized all but one of the S. flexneri 4a strains (O antigens IV:3,4) and all serotype 4 strains (O antigen IV only; IV:-), but neither of the two 4b (O antigens IV:6) strains available |
| Proposed epitopes | α-D-Glcp-1-->6-β-D-GlcpN residues seemed to be the part of the epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria |
| Curator ID | AA + AS |
| Date of Curation | 16-03-2011 |
| References | 2437036 |
| PolysacDB ID | 2278 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype 4a (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
| BCSDB Structure | 4951 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF IV-2 |
| Antibody type and class | IgG2c |
| Assay System | ELISA |
| Cross-reactivity | This Mab cross-reacted with serotype 4b. The MASF IV-2 antibodies agglutinated the two 4b strains, 14 of 20 4a strains (among them the strain that was negative for MASF IV-1), and 19 of 21 serotype 4 strains |
| Proposed epitopes | α-D-Glcp-1-->6-β-D-GlcpN residues seemed to be the part of the epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria |
| Curator ID | AA + AS |
| Date of Curation | 16-03-2011 |
| References | 2437036 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |