PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 17

Entry No. - 1   [TOP]
PolysacDB ID2265
Carbohydrate NameY O-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe O-LPS consists of a tetrasaccharide repeating unit α-L-Rhap-(1-->2)-α-L-Rhap(1-->3)- . This tetrasaccharide is polymerized and joined by β-1,2 linkages between N-acetyl-D-glucosamine and L-rhamnosyl to form the O-polysaccharide chain
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat-killed S. flexnerf bacteria serotype Y
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF Y-1
Antibody type and classN/A
Assay SystemELISA, FARR assay
Cross-reactivityThis Mab bound only to LPS of serotypes 4a and Y. In the glycoconjugate ELISA, MASF Y-1 bound only to the octasaccharide-(cdabcdab') and the decasaccharide-(abcdabcdab') BSA conjugate
Proposed epitopesThis Mab bound to intrachain determinants
IEDB Epitope76764
Proposed UtilityThis Mab would help in the delineation of the antigenic stucture of Y O polysaccharide
Curator IDAA + AS
Date of Curation15-03-2011
References2438343


Entry No. - 2   [TOP]
PolysacDB ID2266
Carbohydrate NameY O-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe O-LPS consists of a tetrasaccharide repeating unit α-L-Rhap-(1-->2)-α-L-Rhap(1-->3)- . This tetrasaccharide is polymerized and joined by β-1,2 linkages between N-acetyl-D-glucosamine and L-rhamnosyl to form the O-polysaccharide chain
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat-killed S. flexnerf bacteria serotype Y
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF Y-2
Antibody type and classN/A
Assay SystemELISA, FARR assay
Cross-reactivityMASF Y-2 bound exclusively to LPS of serotype Y. In the glycoconjugate ELISA. these antibodies bound only to the octa- and decasaccharide-BSA antigens
Proposed epitopesThis Mab bound to intrachain determinants
IEDB Epitope76764
Proposed UtilityThis Mab would help in the delineation of the antigenic stucture of Y O polysaccharide
Curator IDAA + AS
Date of Curation15-03-2011
References2438343


Entry No. - 3   [TOP]
PolysacDB ID2267
Carbohydrate NameY O-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe O-LPS consists of a tetrasaccharide repeating unit α-L-Rhap-(1-->2)-α-L-Rhap(1-->3)- . This tetrasaccharide is polymerized and joined by β-1,2 linkages between N-acetyl-D-glucosamine and L-rhamnosyl to form the O-polysaccharide chain
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat-killed S. flexnerf bacteria serotype Y
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF Y-3
Antibody type and classN/A
Assay SystemELISA, FARR assay
Cross-reactivityMASF Y-3 bound to LPS of serotypes 2a. 3b. 4b. And Y
Proposed epitopesThe terminal nonreducing end of the O-polysaccharide complementary to the tetrasaccharide repeating unit
IEDB Epitope76696
Proposed UtilityThis Mab would help in the delineation of the antigenic stucture of Y O polysaccharide
Curator IDAA + AS
Date of Curation15-03-2011
References2438343


Entry No. - 4   [TOP]
PolysacDB ID2268
Carbohydrate NameY O-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe O-LPS consists of a tetrasaccharide repeating unit α-L-Rhap-(1-->2)-α-L-Rhap(1-->3)- . This tetrasaccharide is polymerized and joined by β-1,2 linkages between N-acetyl-D-glucosamine and L-rhamnosyl to form the O-polysaccharide chain
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat-killed S. flexnerf bacteria serotype Y
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF Y-4
Antibody type and classN/A
Assay SystemELISA, FARR assay
Cross-reactivityMASF Y-4 bound to LPS of serotypes 2a. 3b, 4a, 4b, 5a, and Y
Proposed epitopesThe terminal nonreducing end of the O-polysaccharide complementary to the tetrasaccharide repeating unit
IEDB Epitope76696
Proposed UtilityThis Mab would help in the delineation of the antigenic stucture of Y O polysaccharide
Curator IDAA + AS
Date of Curation15-03-2011
References2438343


Entry No. - 5   [TOP]
PolysacDB ID2269
Carbohydrate NameY O-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe O-LPS consists of a tetrasaccharide repeating unit α-L-Rhap-(1-->2)-α-L-Rhap(1-->3)- . This tetrasaccharide is polymerized and joined by β-1,2 linkages between N-acetyl-D-glucosamine and L-rhamnosyl to form the O-polysaccharide chain
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat-killed S. flexnerf bacteria serotype Y
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF Y-5
Antibody type and classN/A
Assay SystemELISA, FARR assay
Cross-reactivityThe rat Mab (MASF Y-5) bound LPS of serotypes 2a, 3b, 4a, and Y, all carrying the group antigen 3,4. It bound to the tetrasaccharides-( abcd), (bcda), [cdab'), (dabc), the octasaccharide-(cdabcdab'), and the decasaccharide-BSA (abcdabcdab ') glycoconjugates
Proposed epitopesThis Mab bound to intrachain determinants
IEDB Epitope76764
Proposed UtilityThis Mab would help in the delineation of the antigenic stucture of Y O polysaccharide
Curator IDAA + AS
Date of Curation15-03-2011
References2438343


Entry No. - 6   [TOP]
PolysacDB ID2270
Carbohydrate NameY O-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe O-LPS consists of a tetrasaccharide repeating unit α-L-Rhap-(1-->2)-α-L-Rhap(1-->3)- . This tetrasaccharide is polymerized and joined by β-1,2 linkages between N-acetyl-D-glucosamine and L-rhamnosyl to form the O-polysaccharide chain
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat-killed S. flexnerf bacteria serotype Y
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA, FARR assay
Cross-reactivityThe polyclonal rabbit anti-group antigen 3.4 antibodies reacted with the lb, 2a, 3b, 4a. 4b, and Y LPS. They did not bind to1a and 5a. In glycoconjugate ELISA's it bound to all saccharides especially abcd- and decasaccharide- BSA antigens
Proposed epitopesAntigen 3,4 seemed to be the epitope
IEDB Epitope76696
Proposed UtilityThis Mab would help in the delineation of the antigenic stucture of Y O polysaccharide
Curator IDAA + AS
Date of Curation16-03-2011
References2438343


Entry No. - 7   [TOP]
PolysacDB ID2323
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure3707
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed cells
Carrier NameNil
Conjugation MethodNil
AntibodiesAntisera
Antibody type and classN/A
Assay SystemELISA
Cross-reactivityThe highest titers seen were against serotype 3a and 3b LPSs
Proposed epitopesIn S. flexneri serotype 3a and 3b LPSs there is no structural feature to which the type III antigen epitope can be assigned. Instead, the serotype specificity is a consequence of the addition of two group antigen epitopes to the basic structure in serotype 3a (7,8 and 6 epitopes) and of one group antigen epitope to the basic structure in serotype 3b (6 epitope). The α-D-glucose-(1-->3)-α-L-rhamnose was assigned as the determinant for group 7,8 antigen, and the 2-O-acetyl-α-L-rhamnose III structure was assigned as the determinant for group 6 antigen
IEDB Epitope76815
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation02-04-2011
ReferencesPMC96386


Entry No. - 8   [TOP]
PolysacDB ID2326
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure3707
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF B
Antibody type and classIgM
Assay SystemELISA, slide agglutination
Cross-reactivityThis reacted with all 227 strains tested
Proposed epitopesN/A
IEDB Epitope76815
Proposed UtilityThis Mab can be useful for antigen detection in stool samples. Furthermore, use of the panel of monoclonal antibodies made it possible to identify two strains (Y394 and Y400) as putative members of a new provisional serotype of S. flexneri
Curator IDAA + AS
Date of Curation03-04-2011
ReferencesPMC267520


Entry No. - 9   [TOP]
PolysacDB ID2327
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure3707
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF II
Antibody type and classIgM
Assay SystemELISA, slide agglutination
Cross-reactivityThis Mab cross-reacted with serotype 2a and 2b
Proposed epitopesN/A
IEDB Epitope76815
Proposed UtilityThis Mab can be useful for antigen detection in stool samples. Furthermore, use of the panel of monoclonal antibodies made it possible to identify two strains (Y394 and Y400) as putative members of a new provisional serotype of S. flexneri
Curator IDAA + AS
Date of Curation03-04-2011
ReferencesPMC267520


Entry No. - 10   [TOP]
PolysacDB ID2328
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure3707
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF Y-5
Antibody type and classIgM
Assay SystemELISA, slide agglutination
Cross-reactivityThis Mab cross-reacted with serotype 1a, 2a, 3b, 4a and 5a and Y
Proposed epitopesN/A
IEDB Epitope76815
Proposed UtilityThis Mab can be useful for antigen detection in stool samples. Furthermore, use of the panel of monoclonal antibodies made it possible to identify two strains (Y394 and Y400) as putative members of a new provisional serotype of S. flexneri
Curator IDAA + AS
Date of Curation03-04-2011
ReferencesPMC267520


Entry No. - 11   [TOP]
PolysacDB ID2329
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure3707
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF IV-1
Antibody type and classIgG
Assay SystemELISA, slide agglutination
Cross-reactivityThis Mab cross-reacted with serotype 4a, 6, X and Y
Proposed epitopesThis Mab was directed against antigen E1037
IEDB Epitope76815
Proposed UtilityThis Mab can be useful for antigen detection in stool samples. Furthermore, use of the panel of monoclonal antibodies made it possible to identify two strains (Y394 and Y400) as putative members of a new provisional serotype of S. flexneri
Curator IDAA + AS
Date of Curation03-04-2011
ReferencesPMC267520


Entry No. - 12   [TOP]
PolysacDB ID2330
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure3707
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF V
Antibody type and classIgG
Assay SystemELISA, slide agglutination
Cross-reactivityThis Mab cross-reacted with serotype 5a
Proposed epitopesN/A
IEDB Epitope76815
Proposed UtilityThis Mab can be useful for antigen detection in stool samples. Furthermore, use of the panel of monoclonal antibodies made it possible to identify two strains (Y394 and Y400) as putative members of a new provisional serotype of S. flexneri
Curator IDAA + AS
Date of Curation03-04-2011
ReferencesPMC267520


Entry No. - 13   [TOP]
PolysacDB ID2331
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure3707
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF VI
Antibody type and classIgG
Assay SystemELISA, slide agglutination
Cross-reactivityThis Mab cross-reacted with serotype 6
Proposed epitopesN/A
IEDB Epitope76815
Proposed UtilityThis Mab can be useful for antigen detection in stool samples. Furthermore, use of the panel of monoclonal antibodies made it possible to identify two strains (Y394 and Y400) as putative members of a new provisional serotype of S. flexneri
Curator IDAA + AS
Date of Curation04-04-2011
ReferencesPMC267520


Entry No. - 14   [TOP]
PolysacDB ID2332
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure3707
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF 6
Antibody type and classIgG
Assay SystemELISA, slide agglutination
Cross-reactivityThis Mab cross-reacted with serotype 1b, 3a, 3b and 3c
Proposed epitopesN/A
IEDB Epitope76815
Proposed UtilityThis Mab can be useful for antigen detection in stool samples. Furthermore, use of the panel of monoclonal antibodies made it possible to identify two strains (Y394 and Y400) as putative members of a new provisional serotype of S. flexneri
Curator IDAA + AS
Date of Curation04-04-2011
ReferencesPMC267520


Entry No. - 15   [TOP]
PolysacDB ID2333
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure3707
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF 7,8
Antibody type and classIgG
Assay SystemELISA, slide agglutination
Cross-reactivityThis Mab cross-reacted with serotype 2b, 3a and X
Proposed epitopesN/A
IEDB Epitope76815
Proposed UtilityThis Mab can be useful for antigen detection in stool samples. Furthermore, use of the panel of monoclonal antibodies made it possible to identify two strains (Y394 and Y400) as putative members of a new provisional serotype of S. flexneri
Curator IDAA + AS
Date of Curation04-04-2011
ReferencesPMC267520


Entry No. - 16   [TOP]
PolysacDB ID2334
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure3707
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF 1
Antibody type and classIgG1
Assay SystemELISA, slide agglutination
Cross-reactivityThis Mab cross-reacted with serotype 1a and 1b
Proposed epitopesN/A
IEDB Epitope76815
Proposed UtilityThis Mab can be useful for antigen detection in stool samples. Furthermore, use of the panel of monoclonal antibodies made it possible to identify two strains (Y394 and Y400) as putative members of a new provisional serotype of S. flexneri
Curator IDAA + AS
Date of Curation05-04-2011
ReferencesPMC267520


Entry No. - 17   [TOP]
PolysacDB ID2335
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure3707
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF IV-2
Antibody type and classIgG2c
Assay SystemELISA, slide agglutination
Cross-reactivityThis Mab cross-reacted with serotype 4a
Proposed epitopesN/A
IEDB Epitope76815
Proposed UtilityThis Mab can be useful for antigen detection in stool samples. Furthermore, use of the panel of monoclonal antibodies made it possible to identify two strains (Y394 and Y400) as putative members of a new provisional serotype of S. flexneri
Curator IDAA + AS
Date of Curation05-04-2011
ReferencesPMC267520


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