PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 8

Entry No. - 1   [TOP]
PolysacDB ID2339
Carbohydrate NameExopolysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeShewanella colweliana   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Aco1
Antibody type and classIgM
Assay SystemELISA, Immunofluorescence and immunoblotting
Cross-reactivityThis Mab was specific to EPS of Shwenella colweliana
Proposed epitopesThis EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator IDAA + AS
Date of Curation06-04-2011
References7686001


Entry No. - 2   [TOP]
PolysacDB ID2340
Carbohydrate NameExopolysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeShewanella colweliana   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Aco2
Antibody type and classIgG2b
Assay SystemELISA, Immunofluorescence and immunoblotting
Cross-reactivityThis Mab cross-reacted with the closely related species S. benthica
Proposed epitopesThis EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator IDAA + AS
Date of Curation06-04-2011
References7686001


Entry No. - 3   [TOP]
PolysacDB ID2341
Carbohydrate NameExopolysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeShewanella colweliana   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Aco3
Antibody type and classIgG2a
Assay SystemELISA, Immunofluorescence and immunoblotting
Cross-reactivityThis Mab cross-reacted with the closely related species S. benthica
Proposed epitopesThis EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator IDAA + AS
Date of Curation07-04-2011
References7686001


Entry No. - 4   [TOP]
PolysacDB ID2342
Carbohydrate NameExopolysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeShewanella colweliana   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Aco4
Antibody type and classIgG2b
Assay SystemELISA, Immunofluorescence and immunoblotting
Cross-reactivityThis Mab was specific to EPS of Shwenella colweliana
Proposed epitopesThis EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator IDAA + AS
Date of Curation07-04-2011
References7686001


Entry No. - 5   [TOP]
PolysacDB ID2343
Carbohydrate NameExopolysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeShewanella colweliana   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Aco5
Antibody type and classIgG2a
Assay SystemELISA, Immunofluorescence and immunoblotting
Cross-reactivityThis Mab was specific to EPS of Shwenella colweliana
Proposed epitopesThis EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator IDAA + AS
Date of Curation07-04-2011
References7686001


Entry No. - 6   [TOP]
PolysacDB ID2344
Carbohydrate NameExopolysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeShewanella colweliana   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Aco6
Antibody type and classIgG1
Assay SystemELISA, Immunofluorescence and immunoblotting
Cross-reactivityThis Mab was specific to EPS of Shwenella colweliana
Proposed epitopesThis EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator IDAA + AS
Date of Curation07-04-2011
References7686001


Entry No. - 7   [TOP]
PolysacDB ID2345
Carbohydrate NameExopolysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeShewanella colweliana   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Aco22
Antibody type and classIgG3
Assay SystemELISA, Immunofluorescence and immunoblotting
Cross-reactivityThis Mab was specific to EPS of Shwenella colweliana
Proposed epitopesThis EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator IDAA + AS
Date of Curation09-04-2011
References7686001


Entry No. - 8   [TOP]
PolysacDB ID2346
Carbohydrate NameExopolysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeShewanella colweliana   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Aco23
Antibody type and classIgG1
Assay SystemELISA, Immunofluorescence and immunoblotting
Cross-reactivityThis Mab was specific to EPS of Shwenella colweliana
Proposed epitopesThis EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator IDAA + AS
Date of Curation09-04-2011
References7686001


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