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| PolysacDB ID | 1038 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Rhizobium leguminosarum (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS is composed of three parts: (1) lipid A, (2) core polysaccharide, and (3) O antigen. The lipid A region contains two glucosamine sugar derivatives, each with three fatty acids and phosphate or pyrophosphate attached. The core polysaccharide has KDO and attached to lipid A. The side chain O is small polysaccharide chain extending outward from the core, it has several peculiar sugars and varies in composition between bacterial strains. |
| BCSDB Structure | 1686 |
| Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
| Antigenic Nature used to produce antibodies | Mixture of suspension of cell envelop. |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 3 |
| Antibody type and class | N\A |
| Assay System | Western blotting, spot imunoassays, agglutination tests |
| Cross-reactivity | This antibody was cross-reactive with different strains of R.leguminosarum like R.biovars viciae, trifolli and phaseoli. |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | N\A |
| Curator ID | AA + AS |
| Date of Curation | 10-01-2010 |
| References | PMC209711 |
| PolysacDB ID | 2598 |
| Carbohydrate Name | Unknown glycoprotein (Drugpedia) |
| Carbohydrate Class | Glycoprotein |
| Microbe | Rhizobium leguminosarum (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | N/A |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Peribacteroid material |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab AFRC MAC 63 |
| Antibody type and class | N/A |
| Assay System | Immunoelectron microscopy |
| Cross-reactivity | This Mab cross-reacts with the peribacteroid membrane from Pisum sativum, plant plasma membranes and the Golgi bodies, but not the endoplasmic reticulum. This Mab also reacted with peribacteroid membranes from nodules of Vicia and lupin, and with plasma membranes and Golgi membranes from uninfected plant cells, including root tip cells from onion (Allium cepa), indicating that the antigen is highly conserved in the plasma membranes of plant cells |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab should prove useful in characterising the maturation and exchange of material between the Golgi apparatus and the plasma membrane of plant cells in general, quite apart from any specific applications as an immunochemical marker for the peribacteroid membrane in further studies on the Rhizobium-legume interaction |
| Curator ID | AA + AS |
| Date of Curation | 14-07-2011 |
| References | PMC554232 |
| PolysacDB ID | 2599 |
| Carbohydrate Name | Unknown glycoprotein (Drugpedia) |
| Carbohydrate Class | Glycoprotein |
| Microbe | Rhizobium leguminosarum (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | N/A |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Peribacteroid material |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab AFRC MAC 64 |
| Antibody type and class | N/A |
| Assay System | Immunoelectron microscopy |
| Cross-reactivity | This Mab cross-reacts with the peribacteroid membrane from Pisum sativum, plant plasma membranes and the Golgi bodies, but not the endoplasmic reticulum. This Mab also reacted with peribacteroid membranes from nodules of Vicia and lupin, and with plasma membranes and Golgi membranes from uninfected plant cells, including root tip cells from onion (Allium cepa), indicating that the antigen is highly conserved in the plasma membranes of plant cells |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab should prove useful in characterising the maturation and exchange of material between the Golgi apparatus and the plasma membrane of plant cells in general, quite apart from any specific applications as an immunochemical marker for the peribacteroid membrane in further studies on the Rhizobium-legume interaction |
| Curator ID | AA + AS |
| Date of Curation | 15-07-2011 |
| References | PMC554232 |
| PolysacDB ID | 2600 |
| Carbohydrate Name | Unknown glycoprotein (Drugpedia) |
| Carbohydrate Class | Glycoprotein |
| Microbe | Rhizobium leguminosarum (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | N/A |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Peribacteroid material |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab AFRC MAC 65 |
| Antibody type and class | N/A |
| Assay System | Immunoelectron microscopy |
| Cross-reactivity | This Mab cross-reacts with the peribacteroid membrane from Pisum sativum, plant plasma membranes and the Golgi bodies, but not the endoplasmic reticulum. This Mab also reacted with peribacteroid membranes from nodules of Vicia and lupin, and with plasma membranes and Golgi membranes from uninfected plant cells, including root tip cells from onion (Allium cepa), indicating that the antigen is highly conserved in the plasma membranes of plant cells |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab should prove useful in characterising the maturation and exchange of material between the Golgi apparatus and the plasma membrane of plant cells in general, quite apart from any specific applications as an immunochemical marker for the peribacteroid membrane in further studies on the Rhizobium-legume interaction |
| Curator ID | AA + AS |
| Date of Curation | 15-07-2011 |
| References | PMC554232 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |