PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 14
Entry No. - 1 [TOP]

PolysacDB ID	1789
Carbohydrate Name	N-propionylated group B meningococcal polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Neisseria meningitidis (NCBI Taxonomy) (Drugpedia)
Basic Structure	It consists of a homopolymer of α(2-->8)-linked sialic acid residues
BCSDB Structure	N/A
Proposed functions	It is an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	N-propionylated group B meningococcal polysaccharide was combined with freshly purified monomeric tetanus toxoid in a solution of phosphate buffer containing NaBH3CN and incubated at 37°C for 5 days
Antibodies	Mab 13A8
Antibody type and class	IgG1
Assay System	ELISA, bactericidal assays
Cross-reactivity	N/A
Proposed epitopes	The epitope was located only on extended helical segments of the polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab would help in the elucidation of the capsular polysaccharide structure
Curator ID	AA + AS
Date of Curation	01-11-2010
References	PMC2196336

Entry No. - 2 [TOP]

PolysacDB ID	1790
Carbohydrate Name	N-propionylated group B meningococcal polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Neisseria meningitidis (NCBI Taxonomy) (Drugpedia)
Basic Structure	It consists of a homopolymer of α(2-->8)-linked sialic acid residues
BCSDB Structure	N/A
Proposed functions	It is an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	N-propionylated group B meningococcal polysaccharide was combined with freshly purified monomeric tetanus toxoid in a solution of phosphate buffer containing NaBH3CN and incubated at 37°C for 5 days
Antibodies	Mab 6E10
Antibody type and class	IgG1
Assay System	ELISA, bactericidal assays
Cross-reactivity	N/A
Proposed epitopes	The epitope was located only on extended helical segments of the polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab would help in the elucidation of the capsular polysaccharide structure
Curator ID	AA + AS
Date of Curation	01-11-2010
References	PMC2196336

Entry No. - 3 [TOP]

PolysacDB ID	1791
Carbohydrate Name	N-propionylated group B meningococcal polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Neisseria meningitidis (NCBI Taxonomy) (Drugpedia)
Basic Structure	It consists of a homopolymer of α(2-->8)-linked sialic acid residues
BCSDB Structure	N/A
Proposed functions	It is an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	N-propionylated group B meningococcal polysaccharide was combined with freshly purified monomeric tetanus toxoid in a solution of phosphate buffer containing NaBH3CN and incubated at 37°C for 5 days
Antibodies	Mab 13A11
Antibody type and class	IgG1
Assay System	ELISA, bactericidal assays
Cross-reactivity	N/A
Proposed epitopes	The epitope was located only on extended helical segments of the polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab would help in the elucidation of the capsular polysaccharide structure
Curator ID	AA + AS
Date of Curation	02-11-2010
References	PMC2196336

Entry No. - 4 [TOP]

PolysacDB ID	1792
Carbohydrate Name	N-propionylated group B meningococcal polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Neisseria meningitidis (NCBI Taxonomy) (Drugpedia)
Basic Structure	It consists of a homopolymer of α(2-->8)-linked sialic acid residues
BCSDB Structure	N/A
Proposed functions	It is an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	N-propionylated group B meningococcal polysaccharide was combined with freshly purified monomeric tetanus toxoid in a solution of phosphate buffer containing NaBH3CN and incubated at 37°C for 5 days
Antibodies	Mab 13D9
Antibody type and class	IgG2a
Assay System	ELISA, bactericidal assays
Cross-reactivity	N/A
Proposed epitopes	The epitope was located only on extended helical segments of the polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab had bactericidal activity
Curator ID	AA + AS
Date of Curation	02-11-2010
References	PMC2196336

Entry No. - 5 [TOP]

PolysacDB ID	1793
Carbohydrate Name	N-propionylated group B meningococcal polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Neisseria meningitidis (NCBI Taxonomy) (Drugpedia)
Basic Structure	It consists of a homopolymer of α(2-->8)-linked sialic acid residues
BCSDB Structure	N/A
Proposed functions	It is an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	N-propionylated group B meningococcal polysaccharide was combined with freshly purified monomeric tetanus toxoid in a solution of phosphate buffer containing NaBH3CN and incubated at 37°C for 5 days
Antibodies	Mab 6B9
Antibody type and class	IgG2a
Assay System	ELISA, bactericidal assays
Cross-reactivity	N/A
Proposed epitopes	The epitope was located only on extended helical segments of the polysaccharide and also comprised short internal fragments
IEDB Epitope	N/A
Proposed Utility	This Mab would help in the elucidation of the capsular polysaccharide structure
Curator ID	AA + AS
Date of Curation	02-11-2010
References	PMC2196336

Entry No. - 6 [TOP]

PolysacDB ID	1794
Carbohydrate Name	N-propionylated group B meningococcal polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Neisseria meningitidis (NCBI Taxonomy) (Drugpedia)
Basic Structure	It consists of a homopolymer of α(2-->8)-linked sialic acid residues
BCSDB Structure	N/A
Proposed functions	It is an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	N-propionylated group B meningococcal polysaccharide was combined with freshly purified monomeric tetanus toxoid in a solution of phosphate buffer containing NaBH3CN and incubated at 37°C for 5 days
Antibodies	Mab 12C5
Antibody type and class	IgG2b
Assay System	ELISA, bactericidal assays
Cross-reactivity	N/A
Proposed epitopes	The epitope was located only on extended helical segments of the polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab had bactericidal activity
Curator ID	AA + AS
Date of Curation	02-11-2010
References	PMC2196336

Entry No. - 7 [TOP]

PolysacDB ID	1795
Carbohydrate Name	N-propionylated group B meningococcal polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Neisseria meningitidis (NCBI Taxonomy) (Drugpedia)
Basic Structure	It consists of a homopolymer of α(2-->8)-linked sialic acid residues
BCSDB Structure	N/A
Proposed functions	It is an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	N-propionylated group B meningococcal polysaccharide was combined with freshly purified monomeric tetanus toxoid in a solution of phosphate buffer containing NaBH3CN and incubated at 37°C for 5 days
Antibodies	Mab 12E12
Antibody type and class	IgG3
Assay System	ELISA, bactericidal assays
Cross-reactivity	N/A
Proposed epitopes	The epitope was located only on extended helical segments of the polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab had bactericidal activity
Curator ID	AA + AS
Date of Curation	02-11-2010
References	PMC2196336

Entry No. - 8 [TOP]

PolysacDB ID	1796
Carbohydrate Name	N-propionylated group B meningococcal polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Neisseria meningitidis (NCBI Taxonomy) (Drugpedia)
Basic Structure	It consists of a homopolymer of α(2-->8)-linked sialic acid residues
BCSDB Structure	N/A
Proposed functions	It is an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	N-propionylated group B meningococcal polysaccharide was combined with freshly purified monomeric tetanus toxoid in a solution of phosphate buffer containing NaBH3CN and incubated at 37°C for 5 days
Antibodies	Mab 14F10
Antibody type and class	IgG3
Assay System	ELISA, bactericidal assays
Cross-reactivity	N/A
Proposed epitopes	The epitope was located only on extended helical segments of the polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab had bactericidal activity
Curator ID	AA + AS
Date of Curation	03-11-2010
References	PMC2196336

Entry No. - 9 [TOP]

PolysacDB ID	1797
Carbohydrate Name	N-propionylated α(2-->8)-linked sialooligosaccharides (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Neisseria meningitidis (NCBI Taxonomy) (Drugpedia)
Basic Structure	N-propionylated α(2-->8)-linked sialooligosaccharides
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	The oligosaccharide was activated using NaIO4 at 4°C for 60 min and combined with monomeric tetanus toxoid in sodium phosphate buffer containing NaBH3CN and heated at 37°C for 5 days
Antibodies	Mab 10B9
Antibody type and class	IgG1
Assay System	ELISA, bactericidal assays
Cross-reactivity	N/A
Proposed epitopes	The epitope was located on short internal fragments of the polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab would help in the elucidation of the capsular polysaccharide structure
Curator ID	AA + AS
Date of Curation	03-11-2010
References	PMC2196336

Entry No. - 10 [TOP]

PolysacDB ID	1798
Carbohydrate Name	N-propionylated α(2-->8)-linked sialooligosaccharides (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Neisseria meningitidis (NCBI Taxonomy) (Drugpedia)
Basic Structure	N-propionylated α(2-->8)-linked sialooligosaccharides
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	The oligosaccharide was activated using NaIO4 at 4°C for 60 min and combined with monomeric tetanus toxoid in sodium phosphate buffer containing NaBH3CN and heated at 37°C for 5 days
Antibodies	Mab 7C11
Antibody type and class	IgG1
Assay System	ELISA, bactericidal assays
Cross-reactivity	N/A
Proposed epitopes	The epitope was located on short internal fragments of the polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab would help in the elucidation of the capsular polysaccharide structure
Curator ID	AA + AS
Date of Curation	03-11-2010
References	PMC2196336

Entry No. - 11 [TOP]

PolysacDB ID	1799
Carbohydrate Name	N-propionylated α(2-->8)-linked sialooligosaccharides (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Neisseria meningitidis (NCBI Taxonomy) (Drugpedia)
Basic Structure	N-propionylated α(2-->8)-linked sialooligosaccharides
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	The oligosaccharide was activated using NaIO4 at 4°C for 60 min and combined with monomeric tetanus toxoid in sodium phosphate buffer containing NaBH3CN and heated at 37°C for 5 days
Antibodies	Mab 11G1
Antibody type and class	IgG2a
Assay System	ELISA, bactericidal assays
Cross-reactivity	N/A
Proposed epitopes	The epitope was located on short internal fragments of the polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab would help in the elucidation of the capsular polysaccharide structure
Curator ID	AA + AS
Date of Curation	03-11-2010
References	PMC2196336

Entry No. - 12 [TOP]

PolysacDB ID	1800
Carbohydrate Name	N-propionylated α(2-->8)-linked sialooligosaccharides (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Neisseria meningitidis (NCBI Taxonomy) (Drugpedia)
Basic Structure	N-propionylated α(2-->8)-linked sialooligosaccharides
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	The oligosaccharide was activated using NaIO4 at 4°C for 60 min and combined with monomeric tetanus toxoid in sodium phosphate buffer containing NaBH3CN and heated at 37°C for 5 days
Antibodies	Mab 1F1
Antibody type and class	IgG2b
Assay System	ELISA, bactericidal assays
Cross-reactivity	N/A
Proposed epitopes	The epitope was located on short internal fragments of the polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab would help in the elucidation of the capsular polysaccharide structure
Curator ID	AA + AS
Date of Curation	04-11-2010
References	PMC2196336

Entry No. - 13 [TOP]

PolysacDB ID	1801
Carbohydrate Name	N-propionylated α(2-->8)-linked sialooligosaccharides (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Neisseria meningitidis (NCBI Taxonomy) (Drugpedia)
Basic Structure	N-propionylated α(2-->8)-linked sialooligosaccharides
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	The oligosaccharide was activated using NaIO4 at 4°C for 60 min and combined with monomeric tetanus toxoid in sodium phosphate buffer containing NaBH3CN and heated at 37°C for 5 days
Antibodies	Mab 11F3
Antibody type and class	IgG3
Assay System	ELISA, bactericidal assays
Cross-reactivity	N/A
Proposed epitopes	The epitope was located on short internal fragments of the polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab would help in the elucidation of the capsular polysaccharide structure
Curator ID	AA + AS
Date of Curation	04-11-2010
References	PMC2196336

Entry No. - 14 [TOP]

PolysacDB ID	1846
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Neisseria meningitidis (NCBI Taxonomy) (Drugpedia)
Basic Structure	It consists of a homopolymer of α(2-->8)-linked sialic acid residues
BCSDB Structure	10616
Proposed functions	It is an important virulence factor
Antigenic Nature used to produce antibodies	Human granulocytes
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab My-28
Antibody type and class	IgM
Assay System	ELISA, immunoblotting
Cross-reactivity	This Mab reacted with N.meningitidis types 2, 3, 4, 7, 8, and 9
Proposed epitopes	Lacto-N-neotetraose residue seemed to constitute the epitope
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	14-11-2010
References	PMC258927

Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020