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| PolysacDB ID | 2574 |
| Carbohydrate Name | Lipid-A (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Mutant derivatives of Rhizobium leguminosarum 3841 with LPS structures lacking the major O-antigen moiety (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Two different classes (LPS-1 and LPS-2) have been defined. LPS-1 macromolecules are formed from three components, i.e., lipid A, core oligosaccharide, and O-antigen, whereas LPS-2 lacks the O-antigen. Lipid A is normally anchored in the outer side of the outer membrane. In R. leguminosarum, the carbohydrate moiety of lipid A is composed of galacturonic acid, glucosamine, and amino-deoxygluconic acid. These molecules are acylated with diverse hydroxy fatty acids of variable lengths (C14 to C18). There is also a unique 27-hydroxy-octacosanoic acid (27-OH C28:0) which could span the whole lipid bilayer. The core oligosaccharide component is structurally conserved among strains and is built from a trisaccharide and a tetrasaccharide, with galacturonic acid, mannose, galactose, and 3-deoxy-D-mannooctulonic acid constituents. The O-antigen region is composed of a strain-specific oligosaccharide repeating unit which projects beyond the outer membrane and is the most antigenic component of the cell wall |
| BCSDB Structure | N/A |
| Proposed functions | Structural modifications of Rhizobium LPS may play an important role in the adaptation of endosymbiotic rhizobia to the surrounding microenvironment |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab JIM32 |
| Antibody type and class | IgG2b |
| Assay System | Dot immunoassay, immunoblotting |
| Cross-reactivity | This Mab cross-reacted with LPS from strain CE109, a derivative of Rhizobium etli CE3. JIM 32 reacted positively with the LPS from many strains of Rhizobium tested (excluding the Rhizobium meliloti subgroup). JIM 32 did not react with representative strains from Bradyrhizobium, Azorhizobium or other related bacterial species |
| Proposed epitopes | Galacturonic acid residues seemed to be a part of the immunodominanat region |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab could be useful for the elucidation of the structure and biosynthesis of LPS |
| Curator ID | AA + AS |
| Date of Curation | 03-07-2011 |
| References | PMC178005 |
| PolysacDB ID | 2575 |
| Carbohydrate Name | Core oligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Mutant derivatives of Rhizobium leguminosarum 3841 with LPS structures lacking the major O-antigen moiety (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Two different classes (LPS-1 and LPS-2) have been defined. LPS-1 macromolecules are formed from three components, i.e., lipid A, core oligosaccharide, and O-antigen, whereas LPS-2 lacks the O-antigen. Lipid A is normally anchored in the outer side of the outer membrane. In R. leguminosarum, the carbohydrate moiety of lipid A is composed of galacturonic acid, glucosamine, and amino-deoxygluconic acid. These molecules are acylated with diverse hydroxy fatty acids of variable lengths (C14 to C18). There is also a unique 27-hydroxy-octacosanoic acid (27-OH C28:0) which could span the whole lipid bilayer. The core oligosaccharide component is structurally conserved among strains and is built from a trisaccharide and a tetrasaccharide, with galacturonic acid, mannose, galactose, and 3-deoxy-D-mannooctulonic acid constituents. The O-antigen region is composed of a strain-specific oligosaccharide repeating unit which projects beyond the outer membrane and is the most antigenic component of the cell wall |
| BCSDB Structure | N/A |
| Proposed functions | Structural modifications of Rhizobium LPS may play an important role in the adaptation of endosymbiotic rhizobia to the surrounding microenvironment |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab JIM33 |
| Antibody type and class | IgG2a |
| Assay System | Dot immunoassay, immunoblotting |
| Cross-reactivity | This Mab cross-reacted with LPS from strain CE109, a derivative of Rhizobium etli CE3 |
| Proposed epitopes | Galacturonic acid residues seemed to be a part of the immunodominanat region |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab could be useful for the elucidation of the structure and biosynthesis of LPS |
| Curator ID | AA + AS |
| Date of Curation | 04-07-2011 |
| References | PMC178005 |
| PolysacDB ID | 2576 |
| Carbohydrate Name | Core oligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Mutant derivatives of Rhizobium leguminosarum 3841 with LPS structures lacking the major O-antigen moiety (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Two different classes (LPS-1 and LPS-2) have been defined. LPS-1 macromolecules are formed from three components, i.e., lipid A, core oligosaccharide, and O-antigen, whereas LPS-2 lacks the O-antigen. Lipid A is normally anchored in the outer side of the outer membrane. In R. leguminosarum, the carbohydrate moiety of lipid A is composed of galacturonic acid, glucosamine, and amino-deoxygluconic acid. These molecules are acylated with diverse hydroxy fatty acids of variable lengths (C14 to C18). There is also a unique 27-hydroxy-octacosanoic acid (27-OH C28:0) which could span the whole lipid bilayer. The core oligosaccharide component is structurally conserved among strains and is built from a trisaccharide and a tetrasaccharide, with galacturonic acid, mannose, galactose, and 3-deoxy-D-mannooctulonic acid constituents. The O-antigen region is composed of a strain-specific oligosaccharide repeating unit which projects beyond the outer membrane and is the most antigenic component of the cell wall |
| BCSDB Structure | N/A |
| Proposed functions | Structural modifications of Rhizobium LPS may play an important role in the adaptation of endosymbiotic rhizobia to the surrounding microenvironment |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab JIM34 |
| Antibody type and class | IgM |
| Assay System | Dot immunoassay, immunoblotting |
| Cross-reactivity | This Mab cross-reacted with LPS from strain CE109, a derivative of Rhizobium etli CE3 |
| Proposed epitopes | Galacturonic acid residues seemed to be a part of the immunodominanat region |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab could be useful for the elucidation of the structure and biosynthesis of LPS |
| Curator ID | AA + AS |
| Date of Curation | 05-07-2011 |
| References | PMC178005 |
| PolysacDB ID | 2577 |
| Carbohydrate Name | Lipid-A (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Mutant derivatives of Rhizobium leguminosarum 3841 with LPS structures lacking the major O-antigen moiety (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Two different classes (LPS-1 and LPS-2) have been defined. LPS-1 macromolecules are formed from three components, i.e., lipid A, core oligosaccharide, and O-antigen, whereas LPS-2 lacks the O-antigen. Lipid A is normally anchored in the outer side of the outer membrane. In R. leguminosarum, the carbohydrate moiety of lipid A is composed of galacturonic acid, glucosamine, and amino-deoxygluconic acid. These molecules are acylated with diverse hydroxy fatty acids of variable lengths (C14 to C18). There is also a unique 27-hydroxy-octacosanoic acid (27-OH C28:0) which could span the whole lipid bilayer. The core oligosaccharide component is structurally conserved among strains and is built from a trisaccharide and a tetrasaccharide, with galacturonic acid, mannose, galactose, and 3-deoxy-D-mannooctulonic acid constituents. The O-antigen region is composed of a strain-specific oligosaccharide repeating unit which projects beyond the outer membrane and is the most antigenic component of the cell wall |
| BCSDB Structure | N/A |
| Proposed functions | Structural modifications of Rhizobium LPS may play an important role in the adaptation of endosymbiotic rhizobia to the surrounding microenvironment |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab JIM35 |
| Antibody type and class | IgM |
| Assay System | Dot immunoassay, immunoblotting |
| Cross-reactivity | This Mab cross-reacted with LPS from strain CE109, a derivative of Rhizobium etli CE3 |
| Proposed epitopes | Galacturonic acid residues seemed to be a part of the immunodominanat region |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab could be useful for the elucidation of the structure and biosynthesis of LPS |
| Curator ID | AA + AS |
| Date of Curation | 05-07-2011 |
| References | PMC178005 |
| PolysacDB ID | 2578 |
| Carbohydrate Name | Core oligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Mutant derivatives of Rhizobium leguminosarum 3841 with LPS structures lacking the major O-antigen moiety (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Two different classes (LPS-1 and LPS-2) have been defined. LPS-1 macromolecules are formed from three components, i.e., lipid A, core oligosaccharide, and O-antigen, whereas LPS-2 lacks the O-antigen. Lipid A is normally anchored in the outer side of the outer membrane. In R. leguminosarum, the carbohydrate moiety of lipid A is composed of galacturonic acid, glucosamine, and amino-deoxygluconic acid. These molecules are acylated with diverse hydroxy fatty acids of variable lengths (C14 to C18). There is also a unique 27-hydroxy-octacosanoic acid (27-OH C28:0) which could span the whole lipid bilayer. The core oligosaccharide component is structurally conserved among strains and is built from a trisaccharide and a tetrasaccharide, with galacturonic acid, mannose, galactose, and 3-deoxy-D-mannooctulonic acid constituents. The O-antigen region is composed of a strain-specific oligosaccharide repeating unit which projects beyond the outer membrane and is the most antigenic component of the cell wall |
| BCSDB Structure | N/A |
| Proposed functions | Structural modifications of Rhizobium LPS may play an important role in the adaptation of endosymbiotic rhizobia to the surrounding microenvironment |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab JIM36 |
| Antibody type and class | IgG2c |
| Assay System | Dot immunoassay, immunoblotting |
| Cross-reactivity | This Mab cross-reacted with LPS from strain CE109, a derivative of Rhizobium etli CE3 |
| Proposed epitopes | Galacturonic acid residues seemed to be a part of the immunodominanat region |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab could be useful for the elucidation of the structure and biosynthesis of LPS |
| Curator ID | AA + AS |
| Date of Curation | 06-07-2011 |
| References | PMC178005 |
| PolysacDB ID | 2579 |
| Carbohydrate Name | Lipid-A (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Mutant derivatives of Rhizobium leguminosarum 3841 with LPS structures lacking the major O-antigen moiety (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Two different classes (LPS-1 and LPS-2) have been defined. LPS-1 macromolecules are formed from three components, i.e., lipid A, core oligosaccharide, and O-antigen, whereas LPS-2 lacks the O-antigen. Lipid A is normally anchored in the outer side of the outer membrane. In R. leguminosarum, the carbohydrate moiety of lipid A is composed of galacturonic acid, glucosamine, and amino-deoxygluconic acid. These molecules are acylated with diverse hydroxy fatty acids of variable lengths (C14 to C18). There is also a unique 27-hydroxy-octacosanoic acid (27-OH C28:0) which could span the whole lipid bilayer. The core oligosaccharide component is structurally conserved among strains and is built from a trisaccharide and a tetrasaccharide, with galacturonic acid, mannose, galactose, and 3-deoxy-D-mannooctulonic acid constituents. The O-antigen region is composed of a strain-specific oligosaccharide repeating unit which projects beyond the outer membrane and is the most antigenic component of the cell wall |
| BCSDB Structure | N/A |
| Proposed functions | Structural modifications of Rhizobium LPS may play an important role in the adaptation of endosymbiotic rhizobia to the surrounding microenvironment |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab JIM37 |
| Antibody type and class | IgM |
| Assay System | Dot immunoassay, immunoblotting |
| Cross-reactivity | This Mab cross-reacted with LPS from strain CE109, a derivative of Rhizobium etli CE3 |
| Proposed epitopes | Galacturonic acid residues seemed to be a part of the immunodominanat region |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab could be useful for the elucidation of the structure and biosynthesis of LPS |
| Curator ID | AA + AS |
| Date of Curation | 07-07-2011 |
| References | PMC178005 |
| PolysacDB ID | 2580 |
| Carbohydrate Name | Lipid-A (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Mutant derivatives of Rhizobium leguminosarum 3841 with LPS structures lacking the major O-antigen moiety (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Two different classes (LPS-1 and LPS-2) have been defined. LPS-1 macromolecules are formed from three components, i.e., lipid A, core oligosaccharide, and O-antigen, whereas LPS-2 lacks the O-antigen. Lipid A is normally anchored in the outer side of the outer membrane. In R. leguminosarum, the carbohydrate moiety of lipid A is composed of galacturonic acid, glucosamine, and amino-deoxygluconic acid. These molecules are acylated with diverse hydroxy fatty acids of variable lengths (C14 to C18). There is also a unique 27-hydroxy-octacosanoic acid (27-OH C28:0) which could span the whole lipid bilayer. The core oligosaccharide component is structurally conserved among strains and is built from a trisaccharide and a tetrasaccharide, with galacturonic acid, mannose, galactose, and 3-deoxy-D-mannooctulonic acid constituents. The O-antigen region is composed of a strain-specific oligosaccharide repeating unit which projects beyond the outer membrane and is the most antigenic component of the cell wall |
| BCSDB Structure | N/A |
| Proposed functions | Structural modifications of Rhizobium LPS may play an important role in the adaptation of endosymbiotic rhizobia to the surrounding microenvironment |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab JIM38 |
| Antibody type and class | IgM |
| Assay System | Dot immunoassay, immunoblotting |
| Cross-reactivity | This Mab cross-reacted with LPS from strain CE109, a derivative of Rhizobium etli CE3 |
| Proposed epitopes | Galacturonic acid residues seemed to be a part of the immunodominanat region |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab could be useful for the elucidation of the structure and biosynthesis of LPS |
| Curator ID | AA + AS |
| Date of Curation | 07-07-2011 |
| References | PMC178005 |
| PolysacDB ID | 2581 |
| Carbohydrate Name | Core oligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Mutant derivatives of Rhizobium leguminosarum 3841 with LPS structures lacking the major O-antigen moiety (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Two different classes (LPS-1 and LPS-2) have been defined. LPS-1 macromolecules are formed from three components, i.e., lipid A, core oligosaccharide, and O-antigen, whereas LPS-2 lacks the O-antigen. Lipid A is normally anchored in the outer side of the outer membrane. In R. leguminosarum, the carbohydrate moiety of lipid A is composed of galacturonic acid, glucosamine, and amino-deoxygluconic acid. These molecules are acylated with diverse hydroxy fatty acids of variable lengths (C14 to C18). There is also a unique 27-hydroxy-octacosanoic acid (27-OH C28:0) which could span the whole lipid bilayer. The core oligosaccharide component is structurally conserved among strains and is built from a trisaccharide and a tetrasaccharide, with galacturonic acid, mannose, galactose, and 3-deoxy-D-mannooctulonic acid constituents. The O-antigen region is composed of a strain-specific oligosaccharide repeating unit which projects beyond the outer membrane and is the most antigenic component of the cell wall |
| BCSDB Structure | N/A |
| Proposed functions | Structural modifications of Rhizobium LPS may play an important role in the adaptation of endosymbiotic rhizobia to the surrounding microenvironment |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab JIM39 |
| Antibody type and class | IgG2c |
| Assay System | Dot immunoassay, immunoblotting |
| Cross-reactivity | This Mab cross-reacted with LPS from strain CE109, a derivative of Rhizobium etli CE3 |
| Proposed epitopes | Galacturonic acid residues seemed to be a part of the immunodominanat region |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab could be useful for the elucidation of the structure and biosynthesis of LPS |
| Curator ID | AA + AS |
| Date of Curation | 07-07-2011 |
| References | PMC178005 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |