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| PolysacDB ID | 1621 |
| Carbohydrate Name | Lipooligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Moraxella catarrhalis Serotype A strain 25238 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)-(1-->5)-Kdo |
| BCSDB Structure | N/A |
| Proposed functions | Moracella lipooligosaccharide is a potential virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugation Method | Adipic acid dihydrazide (ADH) was bound to detoxified lipooligosaccharide [LOS] to form adipic hydrazide (AH)-dLOS derivatives, using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) and N-hydroxysulfosuccinimide (sulfo-NHS). dLOS (70 mg) was dissolved in 7 ml of 345 mM ADH (molar ratio of ADH to LOS is 100 to 1, based on an estimated Mr of 3,000 for dLOS). Sulfo-NHS was added to a concentration of 8 mM, the pH was adjusted to 4.8, and EDC was added to a concentration of 0.1 M. The reaction mixture was stirred and maintained at pH 4.8 for 3 h. The reaction mixture was adjusted to pH 7.0 and passed through the G-50 column as described above. The eluate was assayed for carbohydrate and for ADH. The peaks containing both carbohydrate and AH were pooled, freeze-dried, and designated AH-dLOS. AH-dLOS was measured for its composition, using dLOS and ADH as standards |
| Antibodies | Polysera |
| Antibody type and class | N/A |
| Assay System | Double immunodiffusion |
| Cross-reactivity | N/A |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antisera had complementmediated bactericidal activity against the homologous strain and heterologous strains of M. catarrhalis |
| Curator ID | AA + AS |
| Date of Curation | 24-09-2010 |
| References | 9573066 |
| PolysacDB ID | 1623 |
| Carbohydrate Name | Lipooligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Moraxella catarrhalis Serotype A strain 25238 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-Kdo |
| BCSDB Structure | N/A |
| Proposed functions | Moracella lipooligosaccharide is a potential virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Cross-reactive mutant (CRM9) of diphtheria toxin |
| Conjugation Method | Adipic acid dihydrazide (ADH) was bound to detoxified lipooligosaccharide [LOS] to form adipic hydrazide (AH)-dLOS derivatives, using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) and N-hydroxysulfosuccinimide (sulfo-NHS). dLOS (70 mg) was dissolved in 7 ml of 345 mM ADH (molar ratio of ADH to LOS is 100 to 1, based on an estimated Mr of 3,000 for dLOS). Sulfo-NHS was added to a concentration of 8 mM, the pH was adjusted to 4.8, and EDC was added to a concentration of 0.1 M. The reaction mixture was stirred and maintained at pH 4.8 for 3 h. The reaction mixture was adjusted to pH 7.0 and passed through the G-50 column as described above. The eluate was assayed for carbohydrate and for AH. The peaks containing both carbohydrate and AH were pooled, freeze-dried, and designated AH-dLOS. AH-dLOS was measured for its composition, using dLOS and ADH as standards |
| Antibodies | Polysera |
| Antibody type and class | N/A |
| Assay System | Enzyme-linked immunospot assay, ELISA and Bacterial challenge assay |
| Cross-reactivity | N/A |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | The dLOS-CRM vaccine induced a significant bacterial clearance (70 to 90%) of both homologous and heterologous strains in the lungs compared to that observed in the controls |
| Curator ID | AA + AS |
| Date of Curation | 25-09-2010 |
| References | PMC130355 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |